Rabbit Anti-B Raf (phospho T401) Monoclonal Antibody (RCB-P0741)
CAT#: VS-0126-XL99
Developed to bind the phosphorylated Threonine 401 site of B Raf, this rabbit monoclonal facilitates the study of feedback loop regulation. It is a reliable choice for versatile protein detection across WB, ICC/IF, and IHC platforms.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71048/1487_C12_3_selected.jpg
Normal Tissue
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferus ducts.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1328/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_7_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_8_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_6_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000157764-BRAF
❮
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Specifications
- Host Species
- Rabbit
- Specificity
- B Raf (phospho T401)
- Species Reactivity
- Human; Mouse; Rabbit
- Applications
- WB; ICC/IF; IHC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Highly purified using a dedicated Protein A-mediated ligand interaction step to remove non-specific background proteins and contaminants.
- Concentration
- Lot specific
- Storage
- Store at -20˚C
- Shipping
- Ice Packs
Applications
- Application Notes
- WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
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Related Signaling Pathways
cAMP Signaling Pathway
MAPK Signaling Pathway
Related Diseases
Throid Cancer
Cushing Syndrome
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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