Rabbit Anti-Phospho-MTOR (S2448) Recombinant Antibody (clone 1G6) (CAT#: ZG-0474U)
Rabbit Anti-Phospho-MTOR (S2448) Antibody (clone 1G6) displayed high specificity against the MTOR protein of Human.
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Custom Production
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WB
Figure 1 Rabbit Anti-Phospho-MTOR (S2448) Antibody (ZG-0474U) in WB
Western Blot
Positive WB detected in Hela whole cell lysate, A549 whole cell lysate
All lanes Phospho-MTOR antibody at 0.825μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 289 KDa
Observed band size: 289 KDa
IHC
Figure 2 Rabbit Anti-Phospho-MTOR (S2448) Antibody (ZG-0474U) in IHC
IHC image of ZG-0474U diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IF
Figure 3 Rabbit Anti-Phospho-MTOR (S2448) Antibody (ZG-0474U) in IF
Immunofluorescence staining of Hela cells with ZG-0474U at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-MTOR (S2448)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human Phospho-MTOR (S2448)
- Species Reactivity
- Human
- Clone
- 1G6
- Applications
- ELISA, WB, IHC, IF
- Conjugate
- Unconjugated
- Related Disease
- Smith-Kingsmore Syndrome and Focal Cortical Dysplasia, Type Ii.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunofluorescence. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200
Target
- Alternative Names
- Serine/threonine-protein kinase mTOR; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian target of rapamycin; mTOR; Mechanistic target of rapamycin; Rapamycin and FKBP12 target 1; Rapamycin target protein 1; MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
- Gene ID
- 2475
- UniProt ID
- P42345
Related Resources
BCR Signaling Pathway
ErbB Signaling Pathway
Insulin Signaling Pathway
mTOR Signaling Pathway
PI3K-Akt Signaling Pathway
Breast Cancer
Colorectal Cancer
EGFR Tyrosine Kinase Inhibitor Resistance
Gastric Cancer
Non-small Cell Lung Cancer
Hepatocellular Carcinoma
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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