Anti-PLCG1 Antibody Prodrug, Protease Activated (F7)
CAT#: VS-1025-YC108
The Anti-PLCG1 Antibody Prodrug, Protease Activated (F7) is an antibody prodrug comprised of a recombinant mouse antibody targeting human PLCG1, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to PLCG1 protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to PLCG1 effectively. PLCG1 (Phospholipase C Gamma 1) is an enzyme whose activation is an important step in several oncogenic signaling pathways originating from receptor tyrosine kinases (RTKs) and immune receptors. Activating mutations in PLCG1 are found in certain lymphomas and T-cell malignancies, leading to unchecked signaling that promotes cell survival and proliferation.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36681/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human thyroid gland
Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36681/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4277/14030_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4277/14030_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4277/14030_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4277/14030_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4277/14030_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4277/14030_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000124181-PLCG1/subcellular
❮
❯
Specifications
- Host Animal
- Mouse
- Applications
- In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay
- Specificity
- Human PLCG1
- Target
- PLCG1
- Species Reactivity
- Human
- Isotype
- IgG
- Clone
- F7
- Epitope
- RTRVNGDNRL
- Masking Approach
- Affinity mask
- Mask
- A synthetic peptide
- Protease Cleavable Linker
- MMP-cleavable linker
- Protease Substrate
- MMPs
- Purification
- Protein A purified
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target
- Introduction
- The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.
- Alternative Names
- PLCG1; Phospholipase C gamma 1; PLC-γ1
- Gene ID
- 103245987
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Related Signaling Pathways
cAMP Signaling Pathway
ErbB Signaling Pathway
FcεR1 Signaling Pathway
TCR Signaling Pathway
Related Diseases
Non-small Cell Lung Cancer
Morphine Addiction
Downloadable Resources
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Datasheet
MSDS
COA
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See other products for "Clone F7"
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- Product Name
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Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1663z | Mouse Anti-PLCG1 Recombinant Antibody (clone 26H9) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0171CT | Recombinant Mouse anti-Human PLCG1 Monoclonal antibody (EML671) | WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2740 | Hi-Affi™ Recombinant Rabbit Anti-PLCG1 Monoclonal Antibody (DS2740AB) | ICC, WB | IgG |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0249YC | Mouse Anti-PLCG1 Recombinant Antibody (clone F7) | ELISA, IP, WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0249-YC-S(P) | Mouse Anti-PLCG1 Recombinant Antibody (clone F7); scFv Fragment | ELISA, IP, WB | Mouse scFv |
| CAT | Product Name | Application | Type |
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| FAMAB-0249-YC-F(E) | Mouse Anti-PLCG1 Recombinant Antibody (clone F7); Fab Fragment | ELISA, IP, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
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| MRO-1618-CN | Recombinant Rabbit Anti-PLCG1 Monoclonal Antibody (CBACN-589) | WB, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-CJ744 | Rabbit Anti-PLCG1 Recombinant Antibody (VS3-CJ744) | WB, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY395 | Mouse Anti-NHP PLCG1 Recombinant Antibody (clone 3C2D11) | WB, IHC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5466 | Anti-PLCG1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
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