Mouse Anti-PLCG1 Recombinant Antibody (clone 26H9)
CAT#: MOB-1663z
This product is a mouse antibody that recognizes human PLCG1. It can be used in the following applications: WB, ELISA, FCM, ICC, IF, IHC.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36681/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human thyroid gland
Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36681/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4277/14030_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4277/14030_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4277/14030_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4277/14030_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4277/14030_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4277/14030_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000124181-PLCG1/subcellular
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Specifications
- Immunogen
- Human Phospholipase C, Gamma 1
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human PLCG1
- Species Reactivity
- Human
- Clone
- 26H9
- Applications
- WB, ELISA, FC, ICC, IF, IHC
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western blot, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry.
Target
- Alternative Names
- PLCG1; 1-Phosphatidylinositol-4,5-Bisphosphate Phosphodiesterase Gamma 1; Triphosphoinositide Phosphodiesterase; Phosphoinositidase C; Phospholipase C-148; Phosphoinositide Phospholipase C; 1-Phosphatidylinositol-4,5-Bisphosphate Phosphodiesterase Gamma-1
- UniProt ID
- P19174
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CITATIONS
RESOURCES
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Related Signaling Pathways
cAMP Signaling Pathway
ErbB Signaling Pathway
FcεR1 Signaling Pathway
TCR Signaling Pathway
Related Diseases
Non-small Cell Lung Cancer
Morphine Addiction
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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| MOR-2740 | Hi-Affi™ Recombinant Rabbit Anti-PLCG1 Monoclonal Antibody (DS2740AB) | ICC, WB | IgG |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| FAMAB-0249-YC-F(E) | Mouse Anti-PLCG1 Recombinant Antibody (clone F7); Fab Fragment | ELISA, IP, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
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