Hi-Affi™ Recombinant Rabbit Anti-PLCG1 Monoclonal Antibody (TH217DS)
CAT#: MOR-4703
Recombinant Rabbit anti-PLCG1 monoclonal antibody for WB, FCM, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36681/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human thyroid gland
Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/36681/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4277/14030_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4277/14030_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4277/14030_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4277/14030_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4277/14030_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4277/14030_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000124181-PLCG1/subcellular
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Specifications
- Immunogen
- APeptide corresponding to amino acids 777-789 of P19174.
- Host Species
- Rabbit
- Type
- IgG
- Species Reactivity
- Human, Mouse
- Clone
- TH217DS
- Applications
- Used for immunoassay techniques such as: Western Blot; Flow Cytometry
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- > 95% as determined by both RP-HPLC and SDS-PAGE analysis.
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.09% sodium azide
- Storage
- Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.
Applications
- Application Notes
- FC: 5 µg/test; WB: 2 µg/ml
Target
- Alternative Names
- Phospholipase C Gamma 1; Phospholipase C, Gamma 1 (Formerly Subtype 148); Phosphoinositide Phospholipase C-Gamma-1; Phospholipase C-Gamma-1; Phospholipase C-II; PLC-Gamma-1; EC 3.1.4.11; PLC-148; PLC-II; PLC1; 1-Phosphatidylinositol-4,5-Bisphosphate Phosphodiesterase Gamma 1; 1-Phosphatidylinositol-4,5-Bisphosphate Phosphodiesterase Gamma-1; 1-Phosphatidyl-D-Myo-Inositol-4,5-Bisphosphate; Monophosphatidylinositol Phosphodiesterase; Triphosphoinositide Phosphodiesterase; Phosphatidylinositol Phospholipase C; Phosphoinositide Phospholipase C; Inositoltrisphosphohydrolase; Phospholipase C, Gamma 1; Phosphoinositidase C; Phospholipase C-148; PLCgamma1; NCKAP3; PLC148
- Gene ID
- 5335
- UniProt ID
- P19174
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Related Signaling Pathways
cAMP Signaling Pathway
ErbB Signaling Pathway
FcεR1 Signaling Pathway
TCR Signaling Pathway
Related Diseases
Non-small Cell Lung Cancer
Morphine Addiction
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| FAMAB-0249-YC-F(E) | Mouse Anti-PLCG1 Recombinant Antibody (clone F7); Fab Fragment | ELISA, IP, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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