Rabbit Anti-SIRT1 Recombinant Antibody (clone 9E9) (CAT#: ZG-0071U)
The purified Rabbit Anti-SIRT1 Recombinant Antibody (clone 9E9) displayed high specificity against the SIRT1 protein of human.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
IHC
Figure 1 Rabbit Anti-SIRT1 Antibody (ZG-0071U) in IHC
IHC image of ZG-0071U diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IF
Figure 2 Rabbit Anti-SIRT1 Antibody (ZG-0071U) in IF
Immunofluorescence staining of Hela Cells with ZG-0071U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human SIRT1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human SIRT1
- Species Reactivity
- Human
- Clone
- 9E9
- Applications
- ELISA, IHC, IF
- Conjugate
- Unconjugated
- Related Disease
- Aging and Periapical Periodontitis
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Immunofluorescence. Specific conditions should be optimized by the end user.
IHC:1:50-1:200, IF:1:20-1:200
Target
- Alternative Names
- NAD-dependent protein deacetylase sirtuin-1; hSIRT1; EC 3.5.1; Regulatory protein SIR2 homolog 1; SIR2-like protein 1; hSIR2; Cleaved into: SirtT1 75 kDa fragment; 75SirT1; SIRT1; SIR2L1
- Gene ID
- 23411
- UniProt ID
- Q96EB6
Related Resources
Amphetamine Addiction
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0777MZ | Chicken Anti-SIRT1 Polyclonal IgY | IHC, IF, WB | Chicken antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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