Anti-SMAD2 Immunohistochemistry Kit
CAT#: VS-0325-XY2105
The SMAD2 IHC Kit is designed to be user - friendly. All reagents are provided, simplifying the experimental procedure. It is suitable for both paraffin - fixed and frozen tissue samples. The specific antibodies in this kit can effectively detect SMAD2, facilitating research on its function and expression in different biological systems, particularly in relation to cell proliferation, differentiation, and apoptosis.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, nucleoli & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/73546/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in decidual cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/73546/154496_A_3_7_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25507/58406_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000175387-SMAD2
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Specifications
- Application
- IHC
- Size
- 50 Tests
- Species Reactivity
- Human, Mouse, Rat
- Target
- SMAD2
- Primary Antibody
- Mouse Anti-SMAD2 Monoclonal Antibody
- Secondary Antibody
- Goat anti-Mouse Antibody, HRP
- Sample Type
- FFPE tissue; Frozen section tissue
- Kit Storage
- All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.
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Related Diseases
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Related Signaling Pathways
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "SMAD2"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3321 | Hi-Affi™ Recombinant Rabbit Anti-SMAD2 Monoclonal Antibody (DS3321AB) | FC, ICC, IHC-P, IP, WB | IgG |
| MOR-4512 | Hi-Affi™ Recombinant Rabbit Anti-SMAD2 Monoclonal Antibody (TH20DS) | WB, IF, ICC | IgG |
| MOR-4709 | Hi-Affi™ Recombinant Rabbit Anti-SMAD2 Monoclonal Antibody (TH223DS) | WB, IF, ICC, FC, ChIP | IgG |
| MOR-4735 | Hi-Affi™ Recombinant Rabbit Anti-SMAD2 Monoclonal Antibody (TH249DS) | WB, IF, ICC, FC, ChIP, ELISA | IgG |
| MOR-0037-FY | Rabbit Anti-SMAD2 Recombinant Antibody (clone AFY0008) | ICC, IHC-P, WB, Inhib | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2378-CN | Recombinant Rabbit Anti-SMAD2 (phosphorylated Ser250) Monoclonal Antibody (CBACN-664) | WB, FC | Rabbit IgG |
| MRO-2379-CN | Recombinant Rabbit Anti-SMAD2 (phosphorylated Ser255) Monoclonal Antibody (JF0882) | WB, IHC, IP | Rabbit IgG |
| VS3-QX1018 | Mouse Anti-SMAD2 Recombinant Antibody (clone 4D3) | WB, IP, IF, IHC | Mouse IgG1 |
| VS3-QX1019 | Mouse Anti-SMAD2 Recombinant Antibody (clone 6H5-E3-C11) | WB, ICC | Mouse IgG1 |
| VS3-QX1020 | Mouse Anti-SMAD2 Recombinant Antibody (clone 5G7) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY238 | AbPlus™ Anti-SMAD2 Magnetic Beads (CBACN-511) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY484 | Mouse Anti-NHP SMAD2 Recombinant Antibody (VS-1024-XY484) | WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY71 | Human Anti-SMAD2 scFv-Fc Chimera (VS-0125-FY71) | ADCC | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1077 | CytoStream™ Rabbit Anti-SMAD2 Recombinant Antibody (VS13-YC1077) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6716 | Anti-Human SMAD2 Immunohistochemistry Kit | IHC |
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