Rabbit Anti-STAT3 Recombinant Antibody (clone 3A9) (CAT#: ZG-0584U)
Rabbit Anti-STAT3 Antibody (clone 3A9) displayed high specificity against the STAT3 protein of Human.
Specific Inquiry
IHC
Figure 1 Rabbit Anti-STAT3 Antibody (ZG-0584U) in IHC
Western Blot
Positive WB detected in:Hela whole cell lysate, MCF-7 whole cell lysate
All lanes: STAT3 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 89, 88, 84 kDa
Observed band size: 89 kDa
WB
Figure 2 Rabbit Anti-STAT3 Antibody (ZG-0584U) in WB
IHC image of ZG-0584U diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC
Figure 3 Rabbit Anti-STAT3 Antibody (ZG-0584U) in IHC
IHC image of ZG-0584U diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human STAT3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human STAT3
- Species Reactivity
- Human
- Clone
- 3A9
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Hyper-Ige Recurrent Infection Syndrome 1, Autosomal Dominant and Autoimmune Disease, Multisystem, Infantile-Onset, 1.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- Signal transducer and activator of transcription 3; Acute-phase response factor; STAT3; APRF
- Gene ID
- 6774
- UniProt ID
- P40763
Related Resources
Endometrial Cancer
Non-small Cell Lung Cancer
Pancreatic Cancer
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1395z-F(E) | Recombinant Anti-Human STAT3 Antibody Fab Fragment | FC, ELISA, FuncS | Fab |
| HPAB-M0660-YC-F(E) | Human Anti-STAT3 Recombinant Antibody; Fab Fragment (HPAB-M0660-YC-F(E)) | ELISA | Human Fab |
| HPAB-M0661-YC-F(E) | Human Anti-STAT3 Recombinant Antibody; Fab Fragment (HPAB-M0661-YC-F(E)) | ELISA | Human Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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