Human Anti-FGFR2 (clone 4B9) scFv-Fc Chimera
CAT#: VS-0325-FY132
This product is a recombinant antibody construct designed for targeted cancer therapy and research applications. It combines the antigen-binding specificity of a single-chain variable fragment (scFv) with the effector functions and extended half-life of an Fc region. Specifically, it targets the Fibroblast Growth Factor Receptor 2 (FGFR2), a receptor tyrosine kinase often overexpressed in various cancers, including gastric, colorectal, endometrial, ovarian, breast, and lung cancers.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm, cell junctions & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35305/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in parietal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35305/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human skin
Immunohistochemical staining of human skin shows strong ctyoplasmic positivity in keratinocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10886/26036_B_7_1_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10886/26036_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10886/26036_A_7_3.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10886/26036_A_4_6.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10886/26036_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (AN3-CA, BEWO, CACO-2, HaCaT, HAP1, NTERA-2, T-47d)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000066468-FGFR2
❮
❯
Specifications
- Immunogen
- A 1: 1 mixture of human FGFR2 IgD2-IgD3 (Illb) and human FGFR2 IgD2-IgD3 (IIIc) fused with a human Fc moiety at their C-termini.
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Specificity
- Human FGFR2
- Species Reactivity
- Human
- Clone
- 4B9
- Applications
- Inhibition
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- Fibroblast Growth Factor Receptor 2; Keratinocyte Growth Factor Receptor; Bacteria-Expressed Kinase; EC 2.7.10.1; K-SAM; KGFR; BEK; Protein Tyrosine Kinase, Receptor Like 14; BEK Fibroblast Growth Factor Receptor; Craniofacial Dysostosis 1; Jackson-Weiss Syndrome; Pfeiffer Syndrome; Crouzon Syndrome; CD332 Antigen
- Gene ID
- 2263
- UniProt ID
- P21802
- Long Name
- Fibroblast Growth Factor Receptor 2
- Sequence Similarities
- Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
- Cellular Localization
- Cell membrane; Golgi apparatus; Cytoplasmic vesicle
- Post Translation Modifications
- Autophosphorylated.
Binding of FGF family members together with heparan sulfate proteoglycan or heparin promotes receptor dimerization and autophosphorylation on several tyrosine residues.
Autophosphorylation occurs in trans between the two FGFR molecules present in the dimer.
Phosphorylation at Tyr-769 is essential for interaction with PLCG1.
N-glycosylated in the endoplasmic reticulum.
The N-glycan chains undergo further maturation to an Endo H-resistant form in the Golgi apparatus.
Ubiquitinated.
FGFR2 is rapidly ubiquitinated after autophosphorylation, leading to internalization and degradation.
Subject to degradation both in lysosomes and by the proteasome.
Glycosylation at Asn83, Asn228, Asn123, Asn241, Asn265, Asn297, Asn318, and Asn331
Modification sites at PhosphoSitePlus
Glycosylation from GlyConnect FGFR2_HUMAN (1997)
Glycosylation from GlyGen 10 sites, 6 N-linked glycans (4 sites), 1 O-linked glycan (1 site)
- Protein Refseq
- XP_006717771.1; XP_006717773.1; XP_016871409.1; XP_016871410.1; XP_016871413.1
- Function
- FGFR2 (Fibroblast Growth Factor Receptor 2) is a tyrosine kinase receptor involved in cell proliferation, differentiation, and angiogenesis. It plays a critical role in skeletal development and tissue repair, and its mutations are associated with various developmental disorders and cancers.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS-0325-FY132. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Related Diseases
EGFR Tyrosine Kinase Inhibitor Resistance
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "FGFR2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B026(A) | Recombinant Anti-human FGFR2 Intrabody [(D-Arg)9] | IF, FC, FuncS | scFv-(D-Arg)9 |
| IAB-B026(G) | Recombinant Anti-human FGFR2 Intrabody [+36 GFP] | WB, ICC, FuncS | scFv-(+36GFP) |
| IAB-B026(T) | Recombinant Anti-human FGFR2 Intrabody [Tat] | ICC, Neut, FuncS | scFv-Tat |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-284CL | Anti-Human FGFR2 Recombinant Antibody (BAY 1179470) | IF, WB | Antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-057WM | Human Anti-FGFR2 Recombinant Antibody (TAB-057WM) | ELISA, FC, Block | Human IgG1 |
| TAB-057WM-S(P) | Human Anti-FGFR2 Recombinant Antibody; scFv Fragment (TAB-057WM-S(P)) | ELISA, FC, Block | Human scFv |
| TAB-057WM-F(E) | Human Anti-FGFR2 Recombinant Antibody; Fab Fragment (TAB-057WM-F(E)) | ELISA, FC, Block | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-060WM | Anti-Human FGFR2 Recombinant Antibody (Atto-huMab-08) | ELISA, WB, FC | Human Antibody |
| TAB-060WM-S(P) | Anti-Human FGFR2 Recombinant Antibody scFv Fragment (Atto-huMab-08) | ELISA, WB, FC | Human Antibody |
| TAB-061WM-S(P) | Human Anti-FGFR2 Recombinant Antibody; scFv Fragment (TAB-061WM-S(P)) | Neut, WB, ELISA | Human scFv |
| TAB-060WM-F(E) | Anti-Human FGFR2 Recombinant Antibody Fab Fragment (Atto-huMab-08) | ELISA, WB, FC | Human Antibody |
| TAB-061WM-F(E) | Human Anti-FGFR2 Recombinant Antibody; Fab Fragment (TAB-061WM-F(E)) | Neut, WB, ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-055WM-S(P) | Anti-Human FGFR2 Recombinant Antibody scFv Fragment (GP369/4B9) | ELISA, FC | |
| TAB-055WM-F(E) | Anti-Human FGFR2 Recombinant Antibody Fab Fragment (GP369/4B9) | ELISA, FC | |
| TAB-056WM-F(E) | Mouse Anti-FGFR2 Recombinant Antibody; Fab Fragment (TAB-056WM-F(E)) | ELISA, FC, Block | Mouse Fab |
| TAB-058WM-F(E) | Rat Anti-FGFR2 Recombinant Antibody; Fab Fragment (TAB-058WM-F(E)) | ELISA, FC, Neut, Inhib | Rat Fab |
| TAB-059WM-F(E) | Anti-Human FGFR2 Recombinant Antibody Fab Fragment (Atto-MuMab-01) | ELISA, WB, FC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0737CT | Recombinant Mouse anti-Human FGFR2 Monoclonal antibody (EML2262) | ICC, IF, IHC-P, WB | |
| HPAB-AP348-YC | Human Anti-FGFR2 Recombinant Antibody (HPAB-AP348-YC) | ELISA, FC, Neut, ADCC, FuncS | Chimeric (rat/human) IgG |
| HPAB-AP349-YC | Human Anti-FGFR2 Recombinant Antibody (HPAB-AP349-YC) | ELISA, FC, Neut, ADCC, FuncS | Chimeric (rat/human) IgG |
| HPAB-AP350-YC | Rat Anti-FGFR2 Recombinant Antibody (HPAB-AP350-YC) | ELISA, FC, Neut, ADCC, FuncS | Rat IgG |
| HPAB-AP351-YC | Rat Anti-FGFR2 Recombinant Antibody (HPAB-AP351-YC) | ELISA, FC, Neut, ADCC, FuncS | Rat IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-828CQ | Mouse Anti-FGFR2 Recombinant Antibody (clone CBL511) | WB, Neut | Mouse IgG1 |
| NEUT-829CQ | Mouse Anti-FGFR2 Recombinant Antibody (clone CBL512) | WB, FC, CyTOF®, Neut | Mouse IgG1 |
| NEUT-830CQ | Mouse Anti-FGFR2 Recombinant Antibody (clone CBL513) | FC, IHC, CyTOF®, Neut | Mouse IgG1 |
| NEUT-831CQ | Mouse Anti-FGFR2 Recombinant Antibody (NEUT-831CQ) | IHC, Neut, WB | Mouse IgG1 |
| NEUT-832CQ | Mouse Anti-FGFR2 Recombinant Antibody (clone CBL060) | Neut, WB, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1298 | Hi-Affi™ Rabbit Anti-FGFR2 Recombinant Antibody (clone DS1298AB) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0144-YC-S(P) | Human Anti-FGFR2 Recombinant Antibody (clone Hu4B9-65); scFv Fragment | FuncS | Humanized scFv |
| HPAB-0146-YC-S(P) | Human Anti-FGFR2 Recombinant Antibody; scFv Fragment (HPAB-0146-YC-S(P)) | ELISA, FC, Neut | Human scFv |
| HPAB-0147-YC-S(P) | Human Anti-FGFR2 Recombinant Antibody; scFv Fragment (HPAB-0147-YC-S(P)) | ELISA, FC, Neut | Humanized scFv |
| HPAB-0020-FY-S(P) | Mouse Anti-FGFR2 Recombinant Antibody; scFv Fragment (HPAB-0020-FY-S(P)) | ELISA | Mouse scFv |
| HPAB-AP348-YC-S(P) | Rat Anti-FGFR2 Recombinant Antibody; scFv Fragment (HPAB-AP348-YC-S(P)) | ELISA, FC, Neut, ADCC, FuncS | Rat scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-027CN | Human Anti-FGFR2 Recombinant Antibody (clone M048-D01) | ELISA, WB, FC | Human IgG1 |
| NS-027CN-F(E) | Human Anti-FGFR2 Recombinant Antibody (clone M048-D01); Fab Fragment | ELISA, WB, FC | Human Fab |
| NS-027CN-S(P) | Human Anti-FGFR2 Recombinant Antibody (clone M048-D01); scFv Fragment | ELISA, WB, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0674-WJ-F(E) | Human Anti-FGFR2 Recombinant Antibody (clone M047-D08); Fab Fragment | ELISA, FC | Human Fab |
| HPAB-0675-WJ-F(E) | Human Anti-FGFR2 Recombinant Antibody (clone M017-B02); Fab Fragment | ELISA, FC | Human Fab |
| HPAB-0676-WJ-F(E) | Human Anti-FGFR2 Recombinant Antibody (clone M021-H02); Fab Fragment | ELISA, FC | Human Fab |
| HPAB-0677-WJ-F(E) | Human Anti-FGFR2 Recombinant Antibody (clone M054-D03); Fab Fragment | ELISA, FC | Human Fab |
| HPAB-0678-WJ-F(E) | Human Anti-FGFR2 Recombinant Antibody (clone M054-A05); Fab Fragment | ELISA, FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC624 | Recombinant Anti-FGFR2 Vesicular Antibody, EV Displayed (VS-0425-YC624) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2540 | Anti-FGFR2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2541 | Anti-Mouse FGFR2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2542 | Anti-Rat FGFR2 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: ELISA, IHC, FC, WB, IF, IA
Application: ELISA
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, FC, Inhib, FuncS
Application: Neut, ELISA, WB, SPR, IF, EM
Application: WB, IHC-Fr, IHC-P, ICC, IF
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
