Mouse Anti-MMP9 Recombinant Antibody (clone 5G3)
CAT#: VS3-XY1091
Clone 5G3 is a mouse antibody, which reacts with MMP9 protein. This antibody is suitable for MMP9 detection by ELISA, WB, IHC, FC, ICC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/if_selected.jpg
Subcellular Location
Figure 2 IHC staining of human bone marrow
Immunohistochemical staining of human bone marrow shows strong nuclear and cytoplasmic positivity in bone marrow poietic cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/348/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/30_H1_1_blue_red_green.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/31_H1_1_blue_red_green.jpg
Normal Tissue
Figure 5 Testis
Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63909/152375_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63909/152375_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enriched (NB-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100985-MMP9
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Specifications
- Immunogen
- Recombinant fragment of human MMP9 expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- MMP9
- Species Reactivity
- Human
- Clone
- 5G3
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry
Product Property
- Format
- Liquid
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000), Immunohistochemistry (1/200-1/1000), Flow Cytometry (1/200-1/400) and Immunocytochemistry (1/200-1/1000).
Target
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Related Signaling Pathways
TNF Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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|---|---|---|---|
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| PFBC-174 | Mouse Anti-MMP9 Recombinant Antibody Fab Fragment (PFBC-174) | ELISA, Neut | Mouse Fab |
| HPAB-0513LY-F(E) | Mouse Anti-MMP9 Recombinant Antibody (clone M4); Fab Fragment | FC | Mouse Fab |
| HPAB-0514LY-F(E) | Mouse Anti-MMP9 Recombinant Antibody (clone M12); Fab Fragment | FC | Mouse Fab |
| HPAB-M0311-YC-F(E) | Mouse Anti-MMP9 Recombinant Antibody (clone AB0046); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-533CL | Anti-Human MMP9 Recombinant Antibody (TAB-533CL) | WB, Inhib | Antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0824CL | Human Anti-MMP9 Recombinant Antibody (TAB-0824CL) | ELISA | Human IgG4 |
| TAB-0825CL | Human Anti-MMP9 Recombinant Antibody (TAB-0825CL) | ELISA | Human IgG4 |
| TAB-0824CL-S(P) | Human Anti-MMP9 Recombinant Antibody; scFv Fragment (TAB-0824CL-S(P)) | ELISA | Human scFv |
| TAB-0825CL-S(P) | Human Anti-MMP9 Recombinant Antibody; scFv Fragment (TAB-0825CL-S(P)) | ELISA | Human scFv |
| TAB-0824CL-F(E) | Human Anti-MMP9 Recombinant Antibody; Fab Fragment (TAB-0824CL-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0826CL | Mouse Anti-MMP9 Recombinant Antibody (TAB-0826CL) | Inhib, ELISA, IHC | Mouse IgG |
| TAB-0827CL | Mouse Anti-MMP9 Recombinant Antibody (TAB-0827CL) | ELISA | Mouse IgG |
| TAB-0826CL-S(P) | Mouse Anti-MMP9 Recombinant Antibody; scFv Fragment (TAB-0826CL-S(P)) | ELISA | Mouse scFv |
| TAB-0826CL-F(E) | Mouse Anti-MMP9 Recombinant Antibody; Fab Fragment (TAB-0826CL-F(E)) | ELISA | Mouse Fab |
| TAB-0827CL-F(E) | Mouse Anti-MMP9 Recombinant Antibody; Fab Fragment (TAB-0827CL-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0717MZ | Chicken Anti-MMP9 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1748CQ | Recombinant Mouse Anti-MMP9 Antibody (VMC204) | Inhib, IHC-Fr, IP, IHC-P, WB, IHC, ICC | IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2263 | Hi-Affi™ Recombinant Rabbit Anti-MMP9 Monoclonal Antibody (DS2263AB) | FC, IHC-P, WB | IgG |
| MOR-4331 | Hi-Affi™ Recombinant Rabbit Anti-MMP-9 Monoclonal Antibody (SI420DS) | ELISA | IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-002ML | Anti-Human MMP9 Recombinant Antibody (TAB-002ML) | ELISA, IHC, FC, IP, IF, FuncS | IgG4, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0027-YC | Mouse Anti-MMP9 Recombinant Antibody (HPAB-0027-YC) | FC | Mouse IgG2b, κ |
| HPAB-0028-YC | Human Anti-MMP9 Recombinant Antibody (clone G03-VH-GL-V1/ G06-VL-GL1) | Neut | Human IgG4 |
| MRO-2039-CN | Rabbit Anti-MMP9 Polyclonal Antibody (MRO-2039-CN) | WB, IF, IHC, FC | Rabbit IgG |
| HPAB-M0311-YC | Mouse Anti-MMP9 Recombinant Antibody (clone AB0046) | ELISA, FuncS | Mouse IgG1, κ |
| VS3-XY1092 | Mouse Anti-MMP9 Recombinant Antibody (clone 2G3) | WB, IP, IF, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0199-FY-S(P) | Human Anti-MMP9 Recombinant Antibody (clone DX-2802); scFv Fragment | ELISA, FC | Human scFv |
| HPAB-0200-FY-S(P) | Human Anti-MMP9 Recombinant Antibody (clone M0279-A03); scFv Fragment | ELISA, FC | Human scFv |
| HPAB-0513LY-S(P) | Mouse Anti-MMP9 Recombinant Antibody (clone M4); scfv Fragment | FC | Mouse scfv |
| HPAB-0514LY-S(P) | Mouse Anti-MMP9 Recombinant Antibody (clone M12); scfv Fragment | FC | Mouse scfv |
| HPAB-M0311-YC-S(P) | Mouse Anti-MMP9 Recombinant Antibody (clone AB0046); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-002ML | Afuco™ Anti-MMP9 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-002ML) | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX783 | Mouse Anti-MMP9 Recombinant Antibody (clone 331CT17.4.4) | WB | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY101 | Human Anti-MMP9 (clone DX-2802) scFv-Fc Chimera | WB, Inhib | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1357 | Anti-MMP9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4460 | Anti-Mouse MMP9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4461 | Anti-Zebrafish MMP9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4459 | Anti-Human MMP9 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC735 | CytoStream™ Rabbit Anti-MMP9 Recombinant Antibody (VS13-YC735) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-YC228 | Recombinant Anti-MMP9 Eliminating Antibody, pH-Sensitive (VS-0625-YC228) | Antigen-Sweeping In Vivo. | Human IgG4 kappa |
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