Rabbit Anti-AKT1 (phospho T450) Monoclonal Antibody
CAT#: VS-0126-XL200
Specifically binding to the phosphorylated T450 residue of AKT1, this rabbit monoclonal offers precise recognition of this priming site. It is recommended for comprehensive screening in WB, IHC, and IP.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & microtubules.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/39_F7_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human gall bladder
Immunohistochemical staining of human gall bladder shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong cytoplasmic and nuclear positivity in Purkinje cells and cells in molecular layer.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3765/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & microtubules.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/38_F7_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 5 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & microtubules.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/40_F7_1_red_green.jpg
Normal Tissue
Figure 6 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_B_8_5.jpg
Normal Tissue
Figure 7 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_A_7_3.jpg
Normal Tissue
Figure 8 Liver
Cholangiocytes
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_A_8_4.jpg
Normal Tissue
Figure 11 Lymph node
Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_A_8_8.jpg
RNA Expression
Figure 12 RNA cell line category: Cell line enhanced (MCF7)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000142208-AKT1
Normal Tissue
Figure 9 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_A_9_5.jpg
Normal Tissue
Figure 10 Testis
Elongated or late spermatids
Staining:High
Intensity: Strong
Quantity:>75%
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Peritubular cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Preleptotene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining:High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining:High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2891/7680_A_4_6.jpg
❮
❯
Specifications
- Host Species
- Rabbit
- Antibody Isotype
- Rabbit IgG
- Specificity
- AKT1 (phospho T450)
- Species Reactivity
- Human; Mouse; Rabbit
- Applications
- WB; IHC; IP
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Isolated by exploiting the natural affinity between immobilized ligands and target immunoglobulins during the professional molecular purification workflow process.
- Concentration
- Lot specific
- Storage
- Store at -20˚C
- Shipping
- Ice Packs
Applications
- Application Notes
- WB: 1:1000-1:2000
IHC: 1:50-1:200
IP: 1:50
Target
- Alternative Names
- Protein kinase B alpha; PKB alpha; RAC-alpha serine/threonine-protein kinase
- UniProt ID
- P31749
REVIEWS AND Q&AS
CITATIONS
RESOURCES
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Related Signaling Pathways
BCR Signaling Pathway
ErbB Signaling Pathway
Insulin Signaling Pathway
mTOR Signaling Pathway
PI3K-Akt Signaling Pathway
Ras Signaling Pathway
Related Diseases
Breast Cancer
Colorectal Cancer
Endometrial Cancer
Gastric Cancer
Hepatocellular Carcinoma
Non-small Cell Lung Cancer
Prostate Cancer
Endocrine Resistance
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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