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  • Recombinant Mouse Anti-CEACAM5 Capture Antibody (clone 15A12)

Recombinant Mouse Anti-CEACAM5 Capture Antibody (clone 15A12) (CAT#: VS3-WK1656)

This antibody is a mouse recombinant antibody against human CEACAM5. The sandwich ELISA was established using matched antibody pair 15A12 and 9H4. The clone 15A12 can be used in ELISA analysis as a capture antibody at a dilution of 1/1000. It was developed by immunization of mouse with recombinant human CEACAM5 protein and then was produced as a recombinant antibody. The isotype of this product is mouse IgG1.


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Subcellular Location
Figure 1 IF staining of human cell line HaCaT
Figure 1 IF staining of human cell line HaCaT
(Immunofluorescent staining of human cell line HaCaT shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19758/1660_G5_2_selected.jpg
Figure 2 IHC staining of human duodenum
Figure 2 IHC staining of human duodenum
(Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in a subset of leukocytes.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11041/ihc_selected.jpg
Normal Tissue
Figure 3 Colon
Figure 3 Colon
(Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Gradient
Gradient: Ascending
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21/19_A_7_3.jpg
Figure 4 Rectum
Figure 4 Rectum
(Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21/19_A_6_3.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enriched (OE19)
Figure 5 RNA cell line category: Cell line enriched (OE19)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105388-CEACAM5
Figure 5 RNA cell line category: Cell line enriched (OE19)
Figure 5 RNA cell line category: Cell line enriched (OE19)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105388-CEACAM5

Specifications

  • Immunogen
  • Recombinant human CEACAM5 protein
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1
  • Specificity
  • Human CEACAM5
  • Species Reactivity
  • Human
  • Clone
  • 15A12
  • Applications
  • ELISA
  • Conjugate
  • Unconjugated

Product Property

  • Clonality
  • Monoclonal
  • Purification
  • Protein G affinity purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Aggregation
  • < 5%
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • 0.09% sodium azide
  • Storage
  • Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
  • Shipping
  • Ice packs

Applications

  • Application Notes
  • ELISA: 1/1000

Target

  • Alternative Names
  • Carcinoembryonic Antigen Related Cell Adhesion Molecule 5; Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5; Meconium Antigen 100; CEA; Carcinoembryonic Antigen; CD66e Antigen; CD66e
  • Long Name
  • Carcinoembryonic Antigen Related Cell Adhesion Molecule 5
  • Sequence Similarities
  • Belongs to the immunoglobulin superfamily. CEA family.
  • Cellular Localization
  • Cell membrane, Membrane
  • Post Translation Modifications
  • Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate.
  • Function
  • Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression.
    Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6.
    Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells.
    (Microbial infection) Receptor for E.coli Dr adhesins. Binding of E.coli Dr adhesins leads to dissociation of the homodimer.

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "CEACAM5"

Recombinant Antibody

CAT Product Name Application Type
MOB-1138z Mouse Anti-CEACAM5 Recombinant Antibody (clone 32B10) WB, IP, IF, FC Mouse IgG1, κ
HPAB-0348-YJ Mouse Anti-CEACAM5 Recombinant Antibody (clone 10E1) ELISA, IHC Mouse IgG1
ZG-0391F Mouse Anti-CEACAM5 Recombinant Antibody (ZG-0391F) WB, IF, ELISA Mouse IgG
VS3-CJ445 Mouse Anti-CEACAM5 Recombinant Antibody (VS3-CJ445) WB Mouse IgG
VS3-CJ446 Mouse Anti-CEACAM5 Recombinant Antibody (clone ABT-CEA) IHC-P Mouse IgG2b, κ

Single-domain Antibody

CAT Product Name Application Type
NABL-080 Recombinant Anti-human CEACAM5 VHH Single Domain Antibody WB, ELISA, IHC, FC, FuncS Llama VHH
TAB-002LC-VHH Anti-Human CEACAM5 Recombinant Antibody(VHH library) SPR, FC, ELISA Single domain antibody
HPAB-0204-FY Recombinant llama Anti-CEACAM5 Antibody (HPAB-0204-FY) ADCC, ELISA llama VHH
HPAB-0205-FY Recombinant llama Anti-CEACAM5 Antibody (HPAB-0205-FY) ADCC, E llama VHH
HPAB-0206-FY Recombinant llama Anti-CEACAM5 Antibody (HPAB-0206-FY) ADCC, E llama VHH

Mouse Antibody

CAT Product Name Application Type
TAB-703 Anti-Human CEACAM5 Recombinant Antibody (Altumomab) IF, IP, Neut, FuncS, ELISA, FC, IHC IgG1
TAB-418LC-F(E) Anti-Human CEACAM5 Recombinant Antibody Fab Fragment (COL-Human 9) FC, WB
TAB-419LC-F(E) Anti-Human CEACAM5 Recombinant Antibody Fab Fragment (COL-Human 12) FC, WB
TAB-420LC-F(E) Anti-Human CEACAM5 Recombinant Antibody Fab Fragment (COL-Human 13) FC, WB
TAB-421LC-F(E) Anti-Human CEACAM5 Recombinant Antibody Fab Fragment (COL-Human 15) FC, WB

Immunotoxin

CAT Product Name Application Type
AGTO-L003E anti-CEACAM5 immunotoxin BDI-1 (IgG)-PE Cytotoxicity assay, Functional assay
AGTO-L003R anti-CEACAM5 immunotoxin BDI-1 (IgG)-RTA Cytotoxicity assay, Functional assay
AGTO-L004E anti-CEACAM5 immunotoxin BDI-1 (scFv)-PE Cytotoxicity assay, Functional assay
AGTO-L004R anti-CEACAM5 immunotoxin BDI-1 (scFv)-RTA Cytotoxicity assay, Functional assay

Chimeric Antibody

CAT Product Name Application Type
TAB-409LC Human Anti-CEACAM5 Recombinant Antibody (TAB-409LC) SPR, ELISA, FC Humanized antibody
TAB-412LC Human Anti-CEACAM5 Recombinant Antibody (TAB-412LC) ELISA, FC Chimeric (Mouse/Human) IgG1
TAB-409LC-F(E) Human Anti-CEACAM5 Recombinant Antibody; Fab Fragment (TAB-409LC-F(E)) SPR, ELISA, FC Humanized Fab
TAB-412LC-F(E) Human Anti-CEACAM5 Recombinant Antibody; Fab Fragment (TAB-412LC-F(E)) FC, ELISA Chimeric (Mouse/Human) Fab
TAB-413LC-F(E) Human Anti-CEACAM5 Recombinant Antibody; Fab Fragment (TAB-413LC-F(E)) FC, ELISA Chimeric (Mouse/Human) Fab

Humanized Antibody

CAT Product Name Application Type
TAB-410LC Human Anti-CEACAM5 Recombinant Antibody (TAB-410LC) ELISA Humanized antibody
TAB-414LC Human Anti-CEACAM5 Recombinant Antibody (TAB-414LC) ELISA, FC, Inhib Human IgG1
TAB-415LC Human Anti-CEACAM5 Recombinant Antibody (TAB-415LC) ELISA, FC, Inhib Human IgG1
TAB-416LC Human Anti-CEACAM5 Recombinant Antibody (TAB-416LC) ELISA, FC, Inhib Human IgG1
TAB-417LC Human Anti-CEACAM5 Recombinant Antibody (TAB-417LC) ELISA, FuncS, IF Humanized antibody

Human Antibody

CAT Product Name Application Type
TAB-411LC-S(P) Human Anti-Human CEACAM5 Recombinant Antibody scFv Fragment ELISA Human antibody
TAB-411LC-F(E) Human Anti-Human CEACAM5 Recombinant Antibody Fab Fragment ELISA Human antibody

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0118MZ Chicken Anti-CEACAM5 Polyclonal IgY WB Chicken antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0649 Hi-Affi™ Rabbit Anti-CEACAM5 Recombinant Antibody (clone DS649AB) IHC Rabbit IgG

MHC Tetramer for Cancer

CAT Product Name Application Type
MHC-LC3182 APC-A*02:06/Human CEACAM5 (YLSGANLNL) MHC Tetramer FCM
MHC-LC3183 APC-A*02:01/Human CEACAM5 (YLWWVNNQSL) MHC Tetramer FCM
MHC-LC4537 PE-Mamu-A1*001:01/Human CEACAM5 (RTLTLFNV) MHC Tetramer FCM
MHC-YF053 DRB1*04:01/Human CEA (YACFVSNLATGRNNS) MHC Monomer MHC Multimer
MHC-YF059 DRB1*04:05/Human CEA (LWWVNNQSLPVSP) MHC Monomer MHC Multimer

scFv Fragment Antibody

CAT Product Name Application Type
HPAB-0155-FY-S(P) Mouse Anti-CEACAM5 Recombinant Antibody; scFv Fragment (HPAB-0155-FY-S(P)) Activ Humanized scFv
HPAB-0243-FY-S(P) Mouse Anti-CEACAM5 Recombinant Antibody (clone M5A); scFv Fragment ELISA Mouse scFv
HPAB-0348-YJ-S(P) Mouse Anti-CEACAM5 Recombinant Antibody (clone 10E1); scFv Fragment ELISA, IHC Mouse scFv
HPAB-1094WJ-S(P) Mouse Anti-CEACAM5 Recombinant Antibody; scFv Fragment (HPAB-1094WJ-S(P)) ELISA, FC Mouse scFv
HPAB-2106-FY-S(P) Human Anti-CEACAM5 Recombinant Antibody (clone CEA6); scFv Fragment ELISA, FC, Inhib Human scFv

Fab Fragment Antibody

CAT Product Name Application Type
HPAB-0155-FY-F(E) Mouse Anti-CEACAM5 Recombinant Antibody; Fab Fragment (HPAB-0155-FY-F(E)) Activ Humanized Fab
HPAB-0243-FY-F(E) Human Anti-CEACAM5 Recombinant Antibody (clone M5A); Fab Fragment ELISA Chimeric (mouse/human) Fab
HPAB-0348-YJ-F(E) Mouse Anti-CEACAM5 Recombinant Antibody (clone 10E1); Fab Fragment ELISA, IHC Mouse Fab
HPAB-1094WJ-F(E) Mouse Anti-CEACAM5 Recombinant Antibody; Fab Fragment (HPAB-1094WJ-F(E)) ELISA, FC Mouse Fab
HPAB-2106-FY-F(E) Human Anti-CEACAM5 Recombinant Antibody (clone CEA6); Fab Fragment ELISA, FC, Inhib Human Fab, κ

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-703 Afuco™ Anti-CEACAM5 ADCC Recombinant Antibody (Altumomab), ADCC Enhanced IF, IP, Neut, FuncS, ELISA, FC ADCC enhanced antibody

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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