Recombinant Mouse Anti-CEACAM5 Detection Antibody (clone 9H4)
CAT#: VS3-WK1657
This antibody is a mouse recombinant antibody against human CEACAM5. The sandwich ELISA was established using matched antibody pair 15A12 and 9H4. The clone 9H4 can be used in ELISA analysis as a detection antibody at a dilution of 1/1000. It was developed by immunization of mouse with recombinant human CEACAM5 protein and then was produced as a recombinant antibody. The isotype of this product is mouse IgG1.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HaCaT
Immunofluorescent staining of human cell line HaCaT shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19758/1660_G5_2_selected.jpg
Subcellular Location
Figure 2 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in a subset of leukocytes.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11041/ihc_selected.jpg
Normal Tissue
Figure 3 Colon
Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Gradient
Gradient: Ascending
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21/19_A_7_3.jpg
Normal Tissue
Figure 4 Rectum
Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21/19_A_6_3.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enriched (OE19)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105388-CEACAM5
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Specifications
- Immunogen
- Recombinant human CEACAM5 protein
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human CEACAM5
- Species Reactivity
- Human
- Clone
- 9H4
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.01% thimerosal
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- ELISA: 1/1000
Target
- Alternative Names
- Carcinoembryonic Antigen Related Cell Adhesion Molecule 5; Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5; Meconium Antigen 100; CEA; Carcinoembryonic Antigen; CD66e Antigen; CD66e
- Gene ID
- 1048
- UniProt ID
- P06731
- Long Name
- Carcinoembryonic Antigen Related Cell Adhesion Molecule 5
- Sequence Similarities
- Belongs to the immunoglobulin superfamily. CEA family.
- Cellular Localization
- Cell membrane, Membrane
- Post Translation Modifications
- Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate.
- Protein Refseq
- NP_001278413.1; NP_001295327.1; NP_004354.3
- Function
- Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression.
Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6.
Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells.
(Microbial infection) Receptor for E.coli Dr adhesins. Binding of E.coli Dr adhesins leads to dissociation of the homodimer.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Capture antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1138z | Mouse Anti-CEACAM5 Recombinant Antibody (clone 32B10) | WB, IP, IF, FC | Mouse IgG1, κ |
| FAMAB-1027CQ | Human Anti-CEACAM5 Recombinant Antibody (clone EB-022) | ELISA | Human IgG1 |
| FAMAB-1028CQ | Human Anti-CEACAM5 Recombinant Antibody (clone EB-011) | ELISA | Human IgG1 |
| VS3-QX225 | Mouse Anti-CEACAM5 Recombinant Antibody (clone 1C7) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-L003E | anti-CEACAM5 immunotoxin BDI-1 (IgG)-PE | Cytotoxicity assay, Functional assay | |
| AGTO-L003R | anti-CEACAM5 immunotoxin BDI-1 (IgG)-RTA | Cytotoxicity assay, Functional assay | |
| AGTO-L004E | anti-CEACAM5 immunotoxin BDI-1 (scFv)-PE | Cytotoxicity assay, Functional assay | |
| AGTO-L004R | anti-CEACAM5 immunotoxin BDI-1 (scFv)-RTA | Cytotoxicity assay, Functional assay |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-409LC | Human Anti-CEACAM5 Recombinant Antibody (TAB-409LC) | SPR, ELISA, FC | Humanized antibody |
| TAB-412LC | Human Anti-CEACAM5 Recombinant Antibody (TAB-412LC) | ELISA, FC | Chimeric (Mouse/Human) IgG1 |
| TAB-409LC-S(P) | Human Anti-CEACAM5 Recombinant Antibody; scFv Fragment (TAB-409LC-S(P)) | SPR, ELISA, FC | Humanized scFv |
| TAB-412LC-S(P) | Human Anti-CEACAM5 Recombinant Antibody; scFv Fragment (TAB-412LC-S(P)) | FC, ELISA | Human scFv |
| TAB-413LC-S(P) | Human Anti-CEACAM5 Recombinant Antibody; scFv Fragment (TAB-413LC-S(P)) | FC, ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-410LC-S(P) | Human Anti-CEACAM5 Recombinant Antibody; scFv Fragment (TAB-410LC-S(P)) | ELISA | Humanized scFv |
| TAB-414LC-S(P) | Human Anti-CEACAM5 Recombinant Antibody; scFv Fragment (TAB-414LC-S(P)) | FC, ELISA | Human scFv |
| TAB-415LC-S(P) | Human Anti-CEACAM5 Recombinant Antibody; scFv Fragment (TAB-415LC-S(P)) | FC, ELISA | Human scFv |
| TAB-416LC-S(P) | Human Anti-CEACAM5 Recombinant Antibody; scFv Fragment (TAB-416LC-S(P)) | FC, ELISA | Human scFv |
| TAB-417LC-S(P) | Human Anti-CEACAM5 Recombinant Antibody; scFv Fragment (TAB-417LC-S(P)) | IF, ELISA | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-411LC-S(P) | Human Anti-Human CEACAM5 Recombinant Antibody scFv Fragment | ELISA | Human antibody |
| TAB-411LC-F(E) | Human Anti-Human CEACAM5 Recombinant Antibody Fab Fragment | ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-002LC-VHH | Anti-Human CEACAM5 Recombinant Antibody(VHH library) | SPR, FC, ELISA | Single domain antibody |
| HPAB-0204-FY | Recombinant llama Anti-CEACAM5 Antibody (HPAB-0204-FY) | ADCC, ELISA | llama VHH |
| HPAB-0205-FY | Recombinant llama Anti-CEACAM5 Antibody (HPAB-0205-FY) | ADCC, E | llama VHH |
| HPAB-0206-FY | Recombinant llama Anti-CEACAM5 Antibody (HPAB-0206-FY) | ADCC, E | llama VHH |
| HPAB-1024WJ | Recombinant Camelid Anti-CEACAM5 Single Domain Antibody | SPR, Inhib | Camelid VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0118MZ | Chicken Anti-CEACAM5 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0649 | Hi-Affi™ Rabbit Anti-CEACAM5 Recombinant Antibody (clone DS649AB) | IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC2943 | PE-A*03:01/Human CEACAM5 (HLFGYSWYK) MHC Tetramer | FCM | |
| MHC-LC2944 | PE-A*11:01/Human CEACAM5 (HLFGYSWYK) MHC Tetramer | FCM | |
| MHC-LC2945 | PE-A*31:01/Human CEACAM5 (HLFGYSWYK) MHC Tetramer | FCM | |
| MHC-YF053 | DRB1*04:01/Human CEA (YACFVSNLATGRNNS) MHC Monomer | MHC Multimer | |
| MHC-YF059 | DRB1*04:05/Human CEA (LWWVNNQSLPVSP) MHC Monomer | MHC Multimer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0155-FY-S(P) | Mouse Anti-CEACAM5 Recombinant Antibody; scFv Fragment (HPAB-0155-FY-S(P)) | Activ | Humanized scFv |
| HPAB-0243-FY-S(P) | Mouse Anti-CEACAM5 Recombinant Antibody scFv Fragment (HPAB-0243-FY-S(P)) | ELISA | Mouse scFv |
| HPAB-0348-YJ-S(P) | Mouse Anti-CEACAM5 Recombinant Antibody (clone 10E1); scFv Fragment | ELISA, IHC | Mouse scFv |
| HPAB-1094WJ-S(P) | Mouse Anti-CEACAM5 Recombinant Antibody; scFv Fragment (HPAB-1094WJ-S(P)) | ELISA, FC | Mouse scFv |
| HPAB-2106-FY-S(P) | Human Anti-CEACAM5 Recombinant Antibody (clone CEA6); scFv Fragment | ELISA, FC, Inhib | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0155-FY-F(E) | Mouse Anti-CEACAM5 Recombinant Antibody; Fab Fragment (HPAB-0155-FY-F(E)) | Activ | Humanized Fab |
| HPAB-0243-FY-F(E) | Human Anti-CEACAM5 Recombinant Antibody Fab Fragment (HPAB-0243-FY-F(E)) | ELISA | Chimeric (mouse/human) Fab |
| HPAB-0348-YJ-F(E) | Mouse Anti-CEACAM5 Recombinant Antibody (clone 10E1); Fab Fragment | ELISA, IHC | Mouse Fab |
| HPAB-1094WJ-F(E) | Mouse Anti-CEACAM5 Recombinant Antibody; Fab Fragment (HPAB-1094WJ-F(E)) | ELISA, FC | Mouse Fab |
| HPAB-2106-FY-F(E) | Human Anti-CEACAM5 Recombinant Antibody (clone CEA6); Fab Fragment | ELISA, FC, Inhib | Human Fab, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-703 | Afuco™ Anti-CEACAM5 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-703) | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY62 | Mouse Anti-NHP CEACAM5 Recombinant Antibody (clone SPM506) | IHC, IF, FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY29 | Human Anti-CEACAM5 (clone MFE-23) scFv-Fc Chimera | BI | Human IgG1, scFv-Fc |
| VS-0125-FY89 | Human Anti-CEACAM5 (clone A5B7) scFv-Fc Chimera | ELISA | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC97 | Recombinant Anti-CEACAM5 Vesicular Antibody, EV Displayed (VS-0425-YC97) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1355 | Anti-CEACAM5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1356 | Anti-Human CEACAM5 Immunohistochemistry Kit | IHC |
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