Recombinant Rabbit Anti-JUN Antibody (clone R02-1G3)
CAT#: VS3-FY784
This product is a recombinant rabbit antibody that recognizes JUN. This antibody has been reported for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R02-1G3 is specific for human, mouse JUN. The antigen is human c-jun synthetic peptide.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31174/1313_G11_5_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3801/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows moderate nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3801/10121_A_6_2_selected.jpg
Normal Tissue
Figure 4 IHC staining of human fallopian tube
High expression. RNA expression: 314.1 nTPM
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7780/18871_A_8_7_val_selected.jpg
Normal Tissue
Figure 5 IHC staining of human lymph node
Low expression. RNA expression: 52.1 nTPM
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7780/18871_A_8_8_val_selected.jpg
Normal Tissue
Figure 6 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3801/10121_B_7_5.jpg
Normal Tissue
Figure 7 Thyroid gland
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3801/10121_B_2_5.jpg
Normal Tissue
Figure 8 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3801/10121_A_9_3.jpg
Normal Tissue
Figure 9 Liver
Hepatocytes
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3801/10121_A_7_4.jpg
Normal Tissue
Figure 10 Kidney
Distal tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Proximal tubules (cell body)
Staining: Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3801/10121_A_8_5.jpg
Normal Tissue
Figure 11 Testis
Sertoli cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3801/10121_A_5_6.jpg
RNA Expression
Figure 12 RNA cell line category: Cell line enhanced (RH-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000177606-JUN
❮
❯
WB
Figure 1. Recombinant Rabbit Anti-JUN Antibody (clone R02-1G3) in WB.
Western Blot analysis of c-Jun in 3T3 lysates using c-Jun antibody.
❮
❯
Specifications
- Immunogen
- Human c-Jun synthetic peptide.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse JUN
- Species Reactivity
- Human, Mouse
- Clone
- R02-1G3
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 36 kDa; Observed MW: 48,43 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Transcription factor AP-1; Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39
- Gene ID
- 3725
- UniProt ID
- P05412
- Sequence Similarities
- Belongs to the bZIP family. Jun subfamily.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Ubiquitinated by the SCF(FBXW7), leading to its degradation (PubMed:14739463, PubMed:27458189).
Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7 (PubMed:14739463).
Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
Acetylated at Lys-271 by EP300.
- Protein Refseq
- NP_002219.1
- Function
- Transcription factor that recognizes and binds to the AP-1 consensus motif 5'-TGA[GC]TCA-3' (PubMed:10995748, PubMed:22083952).
Heterodimerizes with proteins of the FOS family to form an AP-1 transcription complex, thereby enhancing its DNA binding activity to the AP-1 consensus sequence 5'-TGA[GC]TCA-3' and enhancing its transcriptional activity (By similarity).
Together with FOSB, plays a role in activation-induced cell death of T cells by binding to the AP-1 promoter site of FASLG/CD95L, and inducing its transcription in response to activation of the TCR/CD3 signaling pathway (PubMed:12618758).
Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation (PubMed:17210646).
Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306).
Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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| BRD-0043MZ | Chicken Anti-AP-1 Polyclonal IgY | WB | Chicken antibody |
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| MOR-1902 | Hi-Affi™ Recombinant Rabbit Anti-JUN Monoclonal Antibody (DS1902AB) | IP, WB | IgG |
| CAT | Product Name | Application | Type |
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| MRO-0365-CN | Recombinant Rabbit Anti-JUN Monoclonal Antibody (CBACN-148) | WB, IF, IHC, IP | Rabbit IgG |
| VS3-CJ415 | Rabbit Anti-JUN (phospho S63) Recombinant Antibody (VS3-CJ415) | WB, ICC, IHC | Rabbit IgG |
| VS3-CJ416 | Rabbit Anti-JUN (phospho T91) Recombinant Antibody (VS3-CJ416) | WB, ICC, IF, IHC, IP | Rabbit IgG |
| VS3-XY951 | Mouse Anti-JUN Recombinant Antibody (clone 4H9) | ELISA, WB, IHC, ICC | Mouse IgG1 |
| VS3-XY952 | Mouse Anti-JUN Recombinant Antibody (clone 5B1) | ELISA, WB, IHC, ICC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| VS-0424-XY164 | AbPlus™ Anti-JUN Magnetic Beads (CBACN-148) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
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