Anti-CD30 Antibody-Cytokine Fusion Protein, scFv-Fc (HRS3)-IL12 (CAT#: ACFP-SH124)
The fusion protein is composed of a CD30 binding domain (HRS3-scFv) that is linked via the human IgG hinge-CH2/CH3 domain to human IL12. It was expressed in CHO and purified with affinity chromatography. The immunocytokine retains the ability to bind the CD30 as well as the biological activity of IL12. The antibody-cytokine fusion proteins enhanced the cytolysis of CD30+ tumor cells and IFN-γ secretion by NK cells, and increased the activation of resting NK cells. These results support the concept that the HRS3-scFv-Fc-(IL12) fusion protein could be valuable for the specific immunotherapy of Hodgkin's lymphoma. This immunocytokine was designed for treating Hodgkin's disease (HD) anaplastic large cell lymphoma.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HDLM-2
(Immunofluorescent staining of human cell line HDLM-2 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14823/1962_A8_5_selected.jpg
(Immunofluorescent staining of human cell line HDLM-2 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14823/1962_A8_5_selected.jpg
Figure 2 IHC staining of human testis
(Immunohistochemical staining of human testis shows strong cytoplasmic positivity in a subset of cells in seminiferus ducts.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32081/ihc_selected.jpg
(Immunohistochemical staining of human testis shows strong cytoplasmic positivity in a subset of cells in seminiferus ducts.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32081/ihc_selected.jpg
Figure 3 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows leukocyte positivity in germinal center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32082/ihc_selected.jpg
(Immunohistochemical staining of human tonsil shows leukocyte positivity in germinal center.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32082/ihc_selected.jpg
Figure 4 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows cytoplasmic positivity in leukocytes.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16/ihc_selected.jpg
(Immunohistochemical staining of human tonsil shows cytoplasmic positivity in leukocytes.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16/ihc_selected.jpg
Figure 5 IF staining of human cell line EFO-21
(Immunofluorescent staining of human cell line EFO-21 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14823/1907_B6_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line EFO-21 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14823/1907_B6_1_blue_red_green.jpg
Figure 6 IF staining of human cell line K-562
(Immunofluorescent staining of human cell line K-562 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14823/1993_D10_4_blue_red_green.jpg
(Immunofluorescent staining of human cell line K-562 shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14823/1993_D10_4_blue_red_green.jpg
Figure 7 Colon
(Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16/165_A_8_3.jpg
(Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16/165_A_8_3.jpg
Figure 8 Appendix
(Glandular cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16/165_A_2_2.jpg
(Glandular cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16/165_A_2_2.jpg
Figure 9 RNA cell line category: Cell line enriched (HDLM-2)
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000120949-TNFRSF8
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000120949-TNFRSF8
Figure 9 RNA cell line category: Cell line enriched (HDLM-2)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120949-TNFRSF8
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120949-TNFRSF8
Specifications
- Target
- CD30
- Molecule Class
- scFv-Fc-IL12
- Species Reactivity
- Human
- Expression Host
- CHO
- Clone
- HRS3
- Related Disease
- Hodgkin's disease (HD) anaplastic large cell lymphoma
- Cytokine 1
- IL12
- Cytokines 1 Description
- Interleukin 12 (IL-12) is an interleukin that is naturally produced by dendritic cells, macrophages, neutrophils, and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 is composed of a bundle of four alpha helices. It is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40). The active heterodimer (referred to as 'p70'), and a homodimer of p40 are formed following protein synthesis.
- Cytokine 1 Species
- Human
- Applications
- This product can be used therapeutically to deliver biologically active ligands to a desired tissue.
Target
- Alternative Names
- TNFRSF8; tumor necrosis factor receptor superfamily; member 8; CD30; Ki-1; D1S166E; tumor necrosis factor receptor superfamily member 8; Ki-1 antigen; CD30L receptor; cytokine receptor CD30; lymphocyte activation antigen CD30
- Gene ID
- 943
- UniProt ID
- A5D8T4
Downloads
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Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-144 | Anti-Human CD30 Recombinant Antibody (Iratumumab) | ELISA, FC, IP, FuncS, IF, Neut, ICC | IgG1 - kappa |
Chimeric Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-153 | Chimeric (Mouse/Human) Anti-TNFRSF8 Recombinant Antibody (clone Brentuximab) | Neut, ELISA, IF, IP, FuncS, FC, ICC | Human IgG1, κ |
Immunotoxin
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G062E | Anti-TNFRSF8 immunotoxin Ki-3 (scFv)-PE | Cytotoxicity assay, Function study | |
| AGTO-G062R | Anti-TNFRSF8 immunotoxin Ki-3 (scFv)-RTA | Cytotoxicity assay, Function study | |
| AGTO-L041E | anti-TNFRSF8 immunotoxin Ber-H2 (IgG)-PE | Cytotoxicity assay, Functional assay | |
| AGTO-L041R | anti-TNFRSF8 immunotoxin Ber-H2 (IgG)-RTA | Cytotoxicity assay, Functional assay | |
| AGTO-L041S | anti-TNFRSF8 immunotoxin Ber-H2 (IgG)-Sap | Cytotoxicity assay, Functional assay |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-635CL | Human Anti-TNFRSF8 Recombinant Antibody (TAB-635CL) | ELISA | Human IgG |
| TAB-103LC | Anti-Human TNFRSF8 Recombinant Antibody | WB, FC, ELISA | Humanized antibody |
| TAB-103LC-S(P) | Anti-Human TNFRSF8 Recombinant Antibody scFv Fragment | WB, FC, ELISA | Humanized antibody |
| TAB-103LC-F(E) | Anti-Human TNFRSF8 Recombinant Antibody Fab Fragment | WB, FC, ELISA | Humanized antibody |
Fc Glycosylation
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-139LC | Recombinant Anti-Human TNFRSF8 Antibody (Fc glycosylation/Non fucosylated) | ELISA | Human antibody |
Low- or Non-fucosylated Oligosaccharide
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-139LC-1 | Recombinant Anti-Human TNFRSF8 Antibody (Fc glycosylation/Non fucosylated) | ELISA | Human antibody |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0282MZ | Recombinant Mouse Anti-Human tumor necrosis factor receptor superfamily, member 8 Antibody (clone Cer-H3) | IHC | Mouse antibody |
| HPAB-0329-YC | Mouse Anti-TNFRSF8 Recombinant Antibody (HPAB-0329-YC) | ELISA, FC, Cyt, FuncS, Inhib | Mouse IgG |
| HPAB-0330-YC | Human Anti-TNFRSF8 Recombinant Antibody (HPAB-0330-YC) | ELISA, FC, Cyt, FuncS, Inhib | Chimeric (mouse/human) IgG1, κ |
| HPAB-0331-YC | Mouse Anti-TNFRSF8 Recombinant Antibody (HPAB-0331-YC) | ELISA, FC, Cyt, FuncS, Inhib | Mouse IgG |
| HPAB-0332-YC | Human Anti-TNFRSF8 Recombinant Antibody (HPAB-0332-YC) | FC, Cyt, FuncS | Human IgG1, κ |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0597MZ | Chicken Anti-TNFRSF8 Polyclonal IgY | WB | Chicken antibody |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2165CQ | Hamster Anti-Tnfrsf8 Recombinant Antibody (clone mCD30.1) | FC, Stim, Costim, Block | Hamster IgG1 |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2166CQ | Hamster Anti-Tnfrsf8 Recombinant Antibody (clone 2SH12-5F) | Neut, FC | Hamster IgG1, κ |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3617 | Rabbit Anti-TNFRSF8 Recombinant Antibody (clone DS3617AB) | WB, IHC-P | Rabbit IgG |
| MOR-3618 | Rabbit Anti-TNFRSF8 Recombinant Antibody (clone DS3618AB) | ELISA | Rabbit IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0445-CN-S(P) | Mouse Anti-TNFRSF8 Recombinant Antibody (clone T105); scFv Fragment | WB, ELISA, FC | Mouse scFv |
| HPAB-0447-CN-S(P) | Mouse Anti-TNFRSF8 Recombinant Antibody (clone T405); scFv Fragment | WB, ELISA | Mouse scFv |
| HPAB-0459-WJ-S(P) | Mouse Anti-TNFRSF8 Recombinant Antibody; scFv Fragment (HPAB-0459-WJ-S(P)) | ELISA | Mouse scFv |
| HPAB-1930-FY-F(E) | Human Anti-TNFRSF8 Recombinant Antibody (clone 2H9); scFv Fragment | FC, ELISA | Human scFv |
| HPAB-1931-FY-F(E) | Human Anti-TNFRSF8 Recombinant Antibody (clone 5F11); scFv Fragment | FC, ELISA | Human scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0445-CN-F(E) | Mouse Anti-TNFRSF8 Recombinant Antibody (clone T105); Fab Fragment | WB, ELISA, FC | Mouse Fab |
| HPAB-0447-CN-F(E) | Mouse Anti-TNFRSF8 Recombinant Antibody (clone T405); Fab Fragment | WB, ELISA | Mouse Fab |
| HPAB-0459-WJ-F(E) | Mouse Anti-TNFRSF8 Recombinant Antibody; Fab Fragment (HPAB-0459-WJ-F(E)) | ELISA | Mouse Fab |
| HPAB-1929-FY-S(P) | Human Anti-TNFRSF8 Recombinant Antibody (clone 17G1); Fab Fragment | FC, ELISA | Human Fab |
| FAMAB-0358WJ-F(E) | Human Anti-TNFRSF8 Recombinant Antibody (clone AK30); Fab Fragment | ELISA, WB | Human Fab |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-144 | Afuco™ Anti-TNFRSF8 ADCC Recombinant Antibody (Iratumumab), ADCC Enhanced | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
| AFC-TAB-153 | Afuco™ Anti-TNFRSF8 ADCC Recombinant Antibody (Brentuximab), ADCC Enhanced | Neut, ELISA, IF, IP, FuncS, FC | ADCC enhanced antibody |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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