Anti-CD30 Antibody-Cytokine Fusion Protein, scFv-Fc (HRS3)-IL12

Name: Anti-CD30 Antibody-Cytokine Fusion Protein, scFv-Fc (HRS3)-IL12
SKU: ACFP-SH124
Rating: 5 (1 reviews)

CAT#: ACFP-SH124

The fusion protein is composed of a CD30 binding domain (HRS3-scFv) that is linked via the human IgG hinge-CH2/CH3 domain to human IL12. It was expressed in CHO and purified with affinity chromatography. The immunocytokine retains the ability to bind the CD30 as well as the biological activity of IL12. The antibody-cytokine fusion proteins enhanced the cytolysis of CD30+ tumor cells and IFN-γ secretion by NK cells, and increased the activation of resting NK cells. These results support the concept that the HRS3-scFv-Fc-(IL12) fusion protein could be valuable for the specific immunotherapy of Hodgkin's lymphoma. This immunocytokine was designed for treating Hodgkin's disease (HD) anaplastic large cell lymphoma.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line HDLM-2

Immunofluorescent staining of human cell line HDLM-2 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14823/1962_A8_5_selected.jpg

Subcellular Location Figure 2 IHC staining of human testis

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in a subset of cells in seminiferus ducts.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32081/ihc_selected.jpg

Subcellular Location Figure 3 IHC staining of human tonsil

Immunohistochemical staining of human tonsil shows leukocyte positivity in germinal center.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/32082/ihc_selected.jpg

Subcellular Location Figure 4 IHC staining of human tonsil

Immunohistochemical staining of human tonsil shows cytoplasmic positivity in leukocytes.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16/ihc_selected.jpg

Subcellular Location Figure 5 IF staining of human cell line EFO-21

Immunofluorescent staining of human cell line EFO-21 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14823/1907_B6_1_blue_red_green.jpg

Subcellular Location Figure 6 IF staining of human cell line K-562

Immunofluorescent staining of human cell line K-562 shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14823/1993_D10_4_blue_red_green.jpg

Normal Tissue Figure 7 Colon

Endothelial cells
Staining:

Medium

Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16/165_A_8_3.jpg

Normal Tissue Figure 8 Appendix

Glandular cells
Staining:

Medium

Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16/165_A_2_2.jpg

RNA Expression Figure 9 RNA cell line category: Cell line enriched (HDLM-2)

Cell lines ordered by descending RNA expression order.

RNA Expression Figure 9 RNA cell line category: Cell line enriched (HDLM-2)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120949-TNFRSF8

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Specifications

Target
CD30

Molecule Class
scFv-Fc-IL12

Species Reactivity
Human

Expression Host
CHO

Clone
HRS3

Related Disease
Hodgkin's disease (HD) anaplastic large cell lymphoma

Cytokine 1
IL12

Cytokines 1 Description
Interleukin 12 (IL-12) is an interleukin that is naturally produced by dendritic cells, macrophages, neutrophils, and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 is composed of a bundle of four alpha helices. It is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40). The active heterodimer (referred to as 'p70'), and a homodimer of p40 are formed following protein synthesis.

Cytokine 1 Species
Human

Applications
This product can be used therapeutically to deliver biologically active ligands to a desired tissue.

Target

Alternative Names
TNFRSF8; tumor necrosis factor receptor superfamily; member 8; CD30; Ki-1; D1S166E; tumor necrosis factor receptor superfamily member 8; Ki-1 antigen; CD30L receptor; cytokine receptor CD30; lymphocyte activation antigen CD30

Gene ID
943

UniProt ID
A5D8T4

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Datasheet

Datasheet - ACFP-SH124.pdf

MSDS

MSDS - ACFP-SH124.pdf

COA

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