Human Anti-FAP Recombinant Antibody; Fab Fragment (TAB-024WM-F(E)) (CAT#: TAB-024WM-F(E))
This product is a recombinant human antibody Fab Fragment that recognizes FAP. The antibody was purified by affinity chromatography.
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SDS-PAGE
-1.png)
Figure 1 Anti-FAP Fab Fragment (TAB-024WM-F(E)) in SDS-PAGE
SDS-PAGE analysis of TAB-024WM-F(E) in reduced (lane 1) conditions.
SEC-HPLC
-2.png)
Figure 2 Anti-FAP Fab Fragment (TAB-024WM-F(E)) in HPLC
The purity of TAB-024WM-F(E) was greater than 95% as determined by SEC-HPLC.
Column: 3 µm, 7.8x300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.3
Detection: UV280 nm
Injection: 10 µl
Specifications
- Immunogen
- Human or murine fibroblast activating protein (FAP)
- Host Species
- Human
- Type
- Human Fab
- Specificity
- Human
- Species Reactivity
- Human, Mouse, Monkey
- Applications
- Used for immunoassay techniques such as: Enzyme-linked Immunosorbent Assay; Flow Cytometry; Immunofluorescence
- Related Disease
- Disease characterized by expression of FAP
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- FAPA; SIMP; DPPIV; FAP; fibroblast activation protein alpha
- Gene ID
- 2191
- UniProt ID
- Q12884
Related Resources
Sitia, Leopoldo, et al. "Selective targeting of cancer-associated fibroblasts by engineered H-ferritin nanocages loaded with navitoclax." Cells 10.2 (2021): 328. https://doi.org/10.3390/cells10020328
This research investigates the development of engineered H-Ferritin (HFn) nanocages for targeted delivery of navitoclax (Nav) to cancer-associated fibroblasts (CAFs) in the tumor microenvironment. The study demonstrates that by functionalizing HFn nanocages with fibroblast activation protein (FAP) antibody fragments, they achieved significantly higher binding with FAP-positive CAFs compared to FAP-negative cancer cells. The navitoclax-loaded nanocages (HNav-FAP) showed enhanced cytotoxicity specifically in FAP-positive cells while maintaining the drug's pro-apoptotic mechanism of action. Importantly, in vivo experiments confirmed that HFn-FAP successfully reached tumors and targeted CAFs after intravenous administration in a mouse model of triple-negative breast cancer.
Creative Biolabs provided FAP antibody fragments (CAT#: TAB-024WM-F(E)) that were used to functionalize the HFn nanocages. These antibody fragments enabled the selective targeting of CAFs over cancer cells by recognizing FAP, a cell surface glycoprotein highly expressed in the stroma surrounding epithelial cancers but not in healthy tissues. This targeting capability was fundamental to the study's success in developing a promising therapeutic approach that could enhance specific drug delivery into CAFs while overcoming navitoclax's limitations of hydrophobicity and toxicity, potentially opening new avenues for tumor microenvironment-focused cancer therapy.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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FC Reagents & Kits
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-211 | Anti-Human FAP Recombinant Antibody (TAB-211) | ELISA, IP, FC, FuncS, Neut, IF, WB | IgG1 - kappa |
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