This antibody-cytokine fusion protein was achieved by conjugating/fusing the Anti-HLA-A2.1 IgG to TNF. It was expressed in CHO and purified with affinity chromatography. The immunocytokine retains the ability to bind the HLA-A2.1 as well as the biological activity of TNF. In vitro analysis revealed that the TNF-FuP stimulated cytokine production and cytotoxic activity of monocytes and, to a low degree, of T cells. TNF-FuP significantly accumulated in the tumor, which led to retardation of tumor growth. This immunocytokine was designed for treating tumor.
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