Anti-Human F3 Recombinant Antibody (TAB-473CQ) (CAT#: TAB-473CQ)
Recombinant monoclonal antibody to F3. This product is a human monoclonal antibody that targets human F3.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
Figure 1 Anti-Human F3 Antibody (TAB-473CQ) in WB
Western blot analysis of TAB-473CQ was performed by loading human F3 protein in reduced condition Lane 1, Lane 2 and Lane 3 (0.1 μg, 0.3 μg and 0.6 μg respectively) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-473CQ (2 μg/mL) and HRP-conjugated goat anti-human IgG as a secondary antibody (1: 5000). Chemiluminescent detection was performed.
Figure 2 Anti-Human F3 Antibody (TAB-473CQ) in DB
Dot Blot analysis of TAB-473CQ was performed by coating with human F3 protein.
TAB-473CQ incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000)
NC: Negative control PC: Positive control
Figure 3 Anti-Human F3 Antibody (TAB-473CQ) in ELISA
ELISA analysis of TAB-473CQ was performed by coating with human F3 protein. Then blocked with BSA and incubated with TAB-473CQ. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Derivation
- Human
- Type
- IgG1, κ
- Specificity
- Human F3
- Species Reactivity
- Human
- Applications
- Used for immunoassay techniques such as: Enzyme-Linked Immunosorbent Assay; Immunohistochemistry; Immunofluorescence; Immunoprecipitation; Flow Cytometry; Inhibition
- Conjugate
- Unconjugated
- Related Disease
- Solid tumors
Product Property
- Purity
- > 95% as determined by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Target
- Alternative Names
- F3; coagulation factor III (thromboplastin, tissue factor); TF; TFA; CD142; tissue factor;
- Gene ID
- 2152
- UniProt ID
- P13726
Related Resources
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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HPAB-1000WJ-F(E) | Human Anti-F3 Recombinant Antibody (clone JT C5); Fab Fragment | ELISA, Inhib | Human Fab |
Customer Reviews and Q&As
Broad Application Spectrum
Excellent Sensitivity
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-473CQ, RRID: AB_3111961)
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