Anti-Human F3 Recombinant Antibody (TAB-473CQ) (CAT#: TAB-473CQ)
Recombinant monoclonal antibody to F3. This product is a human monoclonal antibody that targets human F3.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Anti-Human F3 Antibody (TAB-473CQ) in WB
Western blot analysis of TAB-473CQ was performed by loading human F3 protein in reduced condition Lane 1, Lane 2 and Lane 3 (0.1 μg, 0.3 μg and 0.6 μg respectively) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-473CQ (2 μg/mL) and HRP-conjugated goat anti-human IgG as a secondary antibody (1: 5000). Chemiluminescent detection was performed.
DB
Figure 2 Anti-Human F3 Antibody (TAB-473CQ) in DB
Dot Blot analysis of TAB-473CQ was performed by coating with human F3 protein.
TAB-473CQ incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000)
NC: Negative control PC: Positive control
ELISA
Figure 3 Anti-Human F3 Antibody (TAB-473CQ) in ELISA
ELISA analysis of TAB-473CQ was performed by coating with human F3 protein. Then blocked with BSA and incubated with TAB-473CQ. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Derivation
- Human
- Type
- IgG1, κ
- Specificity
- Human F3
- Species Reactivity
- Human
- Applications
- Used for immunoassay techniques such as: Enzyme-Linked Immunosorbent Assay; Immunohistochemistry; Immunofluorescence; Immunoprecipitation; Flow Cytometry; Inhibition
- Conjugate
- Unconjugated
- Related Disease
- Solid tumors
Product Property
- Purity
- > 95% as determined by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Target
- Alternative Names
- F3; coagulation factor III (thromboplastin, tissue factor); TF; TFA; CD142; tissue factor;
- Gene ID
- 2152
- UniProt ID
- P13726
Related Resources
Please refer to Tisotumab Vedotin Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Tisotumab Vedotin.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-468 | Human Anti-F3 Recombinant Antibody (clone 10H10); Fab Fragment | WB, IF, FuncS | Human Fab |
| HPAB-0113-YC-F(E) | Human Anti-F3 Recombinant Antibody; Fab Fragment (HPAB-0113-YC-F(E)) | ELISA, FC, Neut | Human Fab |
| HPAB-0114-YC-F(E) | Mouse Anti-F3 Recombinant Antibody; Fab Fragment (HPAB-0114-YC-F(E)) | ELISA | Mouse Fab |
| HPAB-0117-YC-F(E) | Mouse Anti-F3 Recombinant Antibody (clone H36.D2.B7); Fab Fragment | ELISA, WB | Mouse Fab |
| HPAB-1000WJ-F(E) | Human Anti-F3 Recombinant Antibody (clone JT C5); Fab Fragment | ELISA, Inhib | Human Fab |
Customer Reviews and Q&As
Customer Review
Q&As
Broad Application Spectrum
From IP to flow cytometry, the antibody's broad applicability supports diverse research needs. This versatility enhances our ability to adapt to various experimental designs, significantly broadening the scope of our scientific investigations.
Excellent Sensitivity
The sensitivity of the Anti-Human F3 antibody is exceptional, particularly in ELISA and immunohistochemistry applications. It consistently provides us with high-quality, reproducible data essential for our coagulation studies, ensuring confidence in the results.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-473CQ, RRID: AB_3111961)
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For Research Use Only. Not For Clinical Use.
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