Anti-Human IL1 beta Recombinant Antibody (TAB-161) (CAT#: TAB-161)

Recombinant monoclonal antibody to IL1 beta. This humanized monoclonal antibody is suitable for use in ELISA, FC, IP, FuncS, IF, Neut, ICC and most other immunological methods.

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SDS-PAGE

Figure 1 Anti-Human IL1 beta Recombinant Antibody (TAB-161) in SDS-PAGE

Figure 1 Anti-Human IL1 beta Recombinant Antibody (TAB-161) in SDS-PAGE

SDS-PAGE analysis of TAB-161 in reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

The purity of TAB-161 was greater than 95% as determined by SDS-PAGE.

SEC-HPLC

Figure 2 Anti-Human IL1 beta Recombinant Antibody (TAB-161) in HPLC

Figure 2 Anti-Human IL1 beta Recombinant Antibody (TAB-161) in HPLC

The purity of TAB-161 was greater than 95% as determined by SEC-HPLC.

WB

Figure 3 Anti-Human IL1 beta Recombinant Antibody (TAB-161) in WB

Figure 3 Anti-Human IL1 beta Recombinant Antibody (TAB-161) in WB

Western blot analysis of TAB-161 was performed by loading Recombinant Human IL1 beta protein.

TAB-161 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-human IgG
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)

ELISA

Figure 4 Anti-Human IL1 beta Recombinant Antibody (TAB-161) in ELISA

Figure 4 Anti-Human IL1 beta Recombinant Antibody (TAB-161) in ELISA

ELISA analysis of TAB-161 was performed by coating with Recombinant Human IL1 beta Protein. Then blocked with BSA and incubated with Anti-Human IL1 beta Recombinant Antibody (TAB-161). The Goat Anti-Human IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.


Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • Mouse
  • Derivation
  • Humanized (from mouse)
  • Type
  • IgG2 - kappa
  • Specificity
  • Tested positive against native human antigen.
  • Species Reactivity
  • Human
  • Applications
  • Suitable for use in ELISA, FC, IP, FuncS, IF, Neut, ICC and most other immunological methods.
  • MW
  • 145.2 kDa
  • Related Disease
  • Cardiovascular disease

Product Property

  • Purity
  • >95.0%. Determined by analysis by RP-HPLC & analysis by SDS-PAGE.
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Target

  • Alternative Names
  • IL-1; IL1F2; IL1beta; IL1-BETA

Related Resources

  • Biosimilar Overview
  • Related Diseases
  • Related Signaling Pathways
Please refer to Gevokizumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Gevokizumab.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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(Creative Biolabs Cat# TAB-161, RRID: AB_3111842)

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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