Recombinant monoclonal antibody to IL1 beta. This humanized monoclonal antibody is suitable for use in ELISA, FC, IP, FuncS, IF, Neut, ICC and most other immunological methods.
Figure 1 Anti-Human IL1 beta Recombinant Antibody (TAB-161) in SDS-PAGE
SDS-PAGE analysis of TAB-161 in reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
The purity of TAB-161 was greater than 95% as determined by SDS-PAGE.
Figure 2 Anti-Human IL1 beta Recombinant Antibody (TAB-161) in HPLC
The purity of TAB-161 was greater than 95% as determined by SEC-HPLC.
Figure 3 Anti-Human IL1 beta Recombinant Antibody (TAB-161) in WB
Western blot analysis of TAB-161 was performed by loading Recombinant Human IL1 beta protein.
TAB-161 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-human IgG
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
Figure 4 Anti-Human IL1 beta Recombinant Antibody (TAB-161) in ELISA
ELISA analysis of TAB-161 was performed by coating with Recombinant Human IL1 beta Protein. Then blocked with BSA and incubated with Anti-Human IL1 beta Recombinant Antibody (TAB-161). The Goat Anti-Human IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
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TAB-546LC-F(E) | Human Anti-IL1B Recombinant Antibody; Fab Fragment (TAB-546LC-F(E)) | ELISA, FC | Human Fab |
TAB-547LC-F(E) | Human Anti-IL1B Recombinant Antibody; Fab Fragment (TAB-547LC-F(E)) | ELISA, FC | Human Fab |
TAB-550LC-F(E) | Anti-human IL1B Fab Fragment (9.5.2) | ELISA | Human antibody |
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-161, RRID: AB_3111842)
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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