Recombinant Human Anti-Il-17A Antibody scFv Fragment (CAT-2200) (CAT#: PABX-116-S (P))

Figure 1 IL-17A-induced responses and their inhibition by CAT-2200.

(a) Inhibition of FLAG-tagged IL-17A binding to IL-17RA-Fc using HTRF. Triangles, untagged E.-coli-derived IL-17A; circles, CAT-2200; squares, isotype control antibody. (b) Recombinant E.-coli-derived IL-17A induced IL-8 release from primary human chondrocytes. Mean ± SD for one donor (n= 3). EC50= 0.46 nM. (c) CAT-2200-mediated inhibition of E. coli IL-17A (2 nM) induced IL-8 release from primary human chondrocytes. Mean ± SEM for three donors. Mean IC50= 1.56 nM. (d) CAT-2200-mediated inhibition of T-cellderived IL-17A induced IL-6 release from HT1080 cells compared to isotype control. Mean ± SEM for experiments using different T-cell supernatants.

Gerhardt, S., Abbott, W. M., Hargreaves, D., Pauptit, R. A., Davies, R. A., Needham, M. R., ... & Dawson, S. (2009). Structure of IL-17A in complex with a potent, fully human neutralizing antibody. Journal of molecular biology, 394(5), 905-921.

Figure 2 Wall-eyed stereo representation of the IL-17A epitope recognized by CAT-2200.

Residues from the IL-17A homodimer are shown in pale or dark yellow. Residues from the Fab fragments are shown with the light chain in blue and with the heavy chain in green. Labelled residues allow the interactions listed in Table 2 to be readily located. Residues inbrackets are the equivalent residue in IL-17F. When no residue in brackets is indicated, then it is identical between IL-17A and IL-17F.

Gerhardt, S., Abbott, W. M., Hargreaves, D., Pauptit, R. A., Davies, R. A., Needham, M. R., ... & Dawson, S. (2009). Structure of IL-17A in complex with a potent, fully human neutralizing antibody. Journal of molecular biology, 394(5), 905-921.