Is this antibody capable of neutralizing human IL-17A?

Yes, this human scFv antibody (CAT-2200) can potently neutralize the effects of both recombinant and native human IL-17A. It is suitable for use in functional studies (FuncS) as well as WB and ELISA.

What is the format of this antibody product?

This is a recombinant antibody fragment in the scFv (single-chain variable fragment) format. It is produced in E. coli and has a purity of greater than 95% as determined by SDS-PAGE and HPLC analysis.

Recombinant Human Anti-Il-17A Antibody scFv Fragment (CAT-2200)

CAT#: PABX-116-S (P)

Anti-Il-17A antibody is a Human of scFv class that binds to an Il-17A. This antibody can potently neutralize the effects of recombinant and native human IL-17A.

Published Data Gene Expression

Figure 1 IL-17A-induced responses and their inhibition by CAT-2200.

(a) Inhibition of FLAG-tagged IL-17A binding to IL-17RA-Fc using HTRF. Triangles, untagged E.-coli-derived IL-17A; circles, CAT-2200; squares, isotype control antibody. (b) Recombinant E.-coli-derived IL-17A induced IL-8 release from primary human chondrocytes. Mean ± SD for one donor (n= 3). EC50= 0.46 nM. (c) CAT-2200-mediated inhibition of E. coli IL-17A (2 nM) induced IL-8 release from primary human chondrocytes. Mean ± SEM for three donors. Mean IC50= 1.56 nM. (d) CAT-2200-mediated inhibition of T-cellderived IL-17A induced IL-6 release from HT1080 cells compared to isotype control. Mean ± SEM for experiments using different T-cell supernatants.

Gerhardt, S., Abbott, W. M., Hargreaves, D., Pauptit, R. A., Davies, R. A., Needham, M. R., ... & Dawson, S. (2009). Structure of IL-17A in complex with a potent, fully human neutralizing antibody. Journal of molecular biology, 394(5), 905-921.

Figure 2 Wall-eyed stereo representation of the IL-17A epitope recognized by CAT-2200.

Residues from the IL-17A homodimer are shown in pale or dark yellow. Residues from the Fab fragments are shown with the light chain in blue and with the heavy chain in green. Labelled residues allow the interactions listed in Table 2 to be readily located. Residues inbrackets are the equivalent residue in IL-17F. When no residue in brackets is indicated, then it is identical between IL-17A and IL-17F.

❮

❯

Normal Tissue Figure 1 IHC staining of human small intestine

Immunohistochemical staining of human small intestine shows distinct positivity in peripheral leukocytes.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52258/ihc_selected.jpg

Normal Tissue Figure 2 Cerebral cortex

Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52258/119541_B_7_5.jpg

Normal Tissue Figure 3 Colon

Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52258/119541_A_8_3.jpg

Normal Tissue Figure 4 Liver

Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52258/119541_A_7_4.jpg

Normal Tissue Figure 5 Kidney

Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52258/119541_A_7_5.jpg

Normal Tissue Figure 6 Testis

Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52258/119541_A_4_6.jpg

Normal Tissue Figure 7 Tonsil

Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52258/119541_A_4_8.jpg

RNA Expression Figure 8 RNA cell line category: Group enriched (Karpas-707, U-266/84)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112115-IL17A

❮

❯

Specifications

Immunogen
The details of the immunogen for this antibody are not available.

Host Species
Human

Derivation
Human

Type
scFv

Specificity
Tested positive against native HIV-1

Species Reactivity
Human

Clone
CAT-2200

Applications
WB 1:1000-1:5000
ELISA 1:3000-1:15000
FuncS (assay-depended)

Product Property

Purity
>95% by SDS-PAGE and HPLC analysis

Storage
Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

Application Notes
Assay-depended

Target

Alternative Names
IL17A; interleukin 17A; IL17; CTLA8; IL-17; IL-17A; interleukin-17A; CTLA-8; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8);

Gene ID
3605

UniProt ID
Q16552

REVIEWS AND Q&AS CITATIONS RESOURCES DOWNLOADS RELATED PRODUCTS

Inquiry

Navs

Customer Review

There are currently no Customer reviews or questions for PABX-116-S (P). Click the button above to contact us or submit your feedback about this product.

Breast cancer biomarkers at key points during disease progression

Potent IL-17A Neutralization

This scFv fragment (CAT-2200) is highly effective at neutralizing both recombinant and native human IL-17A. We observed potent inhibition of cytokine effects in our functional assays. It is a valuable tool for studying the inflammatory pathways involving this cytokine.

Versatile For ELISA And WB

We have successfully used this human scFv antibody in both Western Blot and ELISA applications. It detects IL-17A accurately and with high sensitivity. The recombinant production ensures that we get consistent results across different experiments in our inflammation research.

Q&As

Is this antibody capable of neutralizing human IL-17A?

A: Yes, this human scFv antibody (CAT-2200) can potently neutralize the effects of both recombinant and native human IL-17A. It is suitable for use in functional studies (FuncS) as well as WB and ELISA.
What is the format of this antibody product?

A: This is a recombinant antibody fragment in the scFv (single-chain variable fragment) format. It is produced in E. coli and has a purity of greater than 95% as determined by SDS-PAGE and HPLC analysis.

View the frequently asked questions answered by Creative Biolabs Support.

Submit Your Publication

Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.

Related Diseases

Inflammatory Bowel Diseases

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - PABX-116-S (P).pdf

MSDS

MSDS - PABX-116-S (P).pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

See other products for "Clone CAT-2200"

CAT
Product Name

See other products for "IL17A"

Select a product category from the dropdown menu below to view related products.

CAT	Product Name	Application	Type
MOB-1390z	Mouse Anti-IL17A Recombinant Antibody (clone 22B9)	FuncS	Mouse IgG2b

CAT	Product Name	Application	Type
TAB-069	Anti-Human IL17A Recombinant Antibody (TAB-069)	IF, IHC, ELISA, FC	IgG1 - kappa

CAT	Product Name	Application	Type
TAB-H57	Anti-Human IL17A Recombinant Antibody (Perakizumab)	WB, ELISA, IP, FC, FuncS, Neut, IF	IgG1 - kappa

CAT	Product Name	Application	Type
TAB-H08	Anti-Human IL17A/IL17F Recombinant Antibody (TAB-H08)	ELISA, FC, IP, FuncS, IF, Neut, WB	IgG1 - kappa

CAT	Product Name	Application	Type
TAB-399CL	Human Anti-IL17A Recombinant Antibody (TAB-399CL)	ELISA	Human IgG

CAT	Product Name	Application	Type
PABL-237	Human Anti-IL17A Recombinant Antibody (clone CNTO7357)	WB, IHC, FuncS	Human IgG

CAT	Product Name	Application	Type
PABL-238	Human Anti-IL17A Recombinant Antibody (clone Fab6785)	WB, ELISA, FuncS	Human IgG

CAT	Product Name	Application	Type
PSBL-237	Human Anti-IL17A Recombinant Antibody (clone CNTO7357); scFv Fragment	WB, IHC, FuncS	Human scFv

CAT	Product Name	Application	Type
PSBL-238	Human Anti-IL17A Recombinant Antibody (clone Fab6785); scFv Fragment	WB, ELISA, FuncS	Human scFv

CAT	Product Name	Application	Type
PFBL-237	Human Anti-IL17A Recombinant Antibody (clone CNTO7357); Fab Fragment	WB, IHC, FuncS	Human Fab

CAT	Product Name	Application	Type
PFBL-238	Human Anti-IL17A Recombinant Antibody (clone Fab6785); Fab Fragment	WB, ELISA, FuncS	Human Fab

CAT	Product Name	Application	Type
PABW-176	Human Anti-IL17A Recombinant Antibody (PABW-176)	Neut, ELISA, WB	Human IgG

CAT	Product Name	Application	Type
PFBW-176	Human Anti-IL17A Recombinant Antibody; Fab Fragment (PFBW-176)	Neut, ELISA, WB	Human Fab

CAT	Product Name	Application	Type
PSBW-176	Human Anti-IL17A Recombinant Antibody; scFv Fragment (PSBW-176)	Neut, ELISA, WB	Human scFv

CAT	Product Name	Application	Type
TAB-030ZJ	Mouse Anti-IL17A Recombinant Antibody (TAB-030ZJ)	ELISA, Neut	Mouse IgG

CAT	Product Name	Application	Type
TAB-048ZJ	Human Anti-IL17A Recombinant Antibody (TAB-048ZJ)	ELISA, Neut	Humanized antibody

CAT	Product Name	Application	Type
TAB-078ZJ	Human Anti-IL17A Recombinant Antibody (TAB-078ZJ)	ELISA, Block, Neut	Human antibody

CAT	Product Name	Application	Type
TAB-079ZJ	Human Anti-IL17A Recombinant Antibody (TAB-079ZJ)	ELISA, Block, Neut	Human IgG1

CAT	Product Name	Application	Type
TAB-080ZJ	Human Anti-IL17A Recombinant Antibody (TAB-080ZJ)	ELISA, Block, Neut	Human antibody

CAT	Product Name	Application	Type
TAB-030ZJ-S(P)	Mouse Anti-IL17A Recombinant Antibody; scFv Fragment (TAB-030ZJ-S(P))	ELISA, Neut	Mouse scFv

CAT	Product Name	Application	Type
TAB-048ZJ-S(P)	Human Anti-IL17A Recombinant Antibody; scFv Fragment (TAB-048ZJ-S(P))	ELISA, Neut	Human scFv

CAT	Product Name	Application	Type
TAB-078ZJ-S(P)	Human Anti-IL17A Recombinant Antibody; scFv Fragment (TAB-078ZJ-S(P))	ELISA, Block, Neut	Human scFv

CAT	Product Name	Application	Type
TAB-030ZJ-F(E)	Mouse Anti-IL17A Recombinant Antibody; Fab Fragment (TAB-030ZJ-F(E))	ELISA, Neut	Mouse Fab

CAT	Product Name	Application	Type
TAB-048ZJ-F(E)	Human Anti-IL17A Recombinant Antibody; Fab Fragment (TAB-048ZJ-F(E))	ELISA, Neut	Humanized Fab

CAT	Product Name	Application	Type
Gly-077LC	Recombinant Anti-Human IL17A Antibody (Fc glycosylation)	ELISA	Humanized antibody

CAT	Product Name	Application	Type
MOB-1196MZ	Recombinant Mouse Anti-Human IL17A Antibody (clone 2I7)	WB	Mouse antibody

CAT	Product Name	Application	Type
BRD-0289MZ	Chicken Anti-Interleukin-17a Polyclonal IgY	Indirect ELISA, WB	Chicken antibody

CAT	Product Name	Application	Type
NEUT-1202CQ	Mouse Anti-IL17A Recombinant Antibody (NEUT-1202CQ)	Neut	Mouse IgG2a

CAT	Product Name	Application	Type
NEUT-1203CQ	Mouse Anti-IL17A Recombinant Antibody (NEUT-1203CQ)	Neut	Mouse IgG1

CAT	Product Name	Application	Type
NEUT-1204CQ	Mouse Anti-IL17A Recombinant Antibody (clone 403D10.01)	Neut, FC	Mouse IgG1

CAT	Product Name	Application	Type
NEUT-1205CQ	Mouse Anti-IL17A Recombinant Antibody (clone 408H6.01)	Neut, ELISA, FC	Mouse IgG2a

CAT	Product Name	Application	Type
NEUT-1206CQ	Mouse Anti-IL17A Recombinant Antibody (clone 409H7.05)	Neut, FC	Mouse IgG1

CAT	Product Name	Application	Type
HPAB-0061-YC-S(P)	Human Anti-IL17A Recombinant Antibody; scFv Fragment (HPAB-0061-YC-S(P))	ELISA, Neut	Humanized scFv

CAT	Product Name	Application	Type
HPAB-0063-YC-S(P)	Human Anti-IL17A Recombinant Antibody (clone Antibody 7); scFv Fragment	FuncS	Human scFv

CAT	Product Name	Application	Type
HPAB-0061-YC-F(E)	Human Anti-IL17A Recombinant Antibody; Fab Fragment (HPAB-0061-YC-F(E))	ELISA, Neut	Humanized Fab

CAT	Product Name	Application	Type
HPAB-0063-YC-F(E)	Human Anti-IL17A Recombinant Antibody (clone Antibody 7); Fab Fragment	FuncS	Human Fab

CAT	Product Name	Application	Type
NS-042CN	Mouse Anti-IL17A Recombinant Antibody (NS-042CN)	ELISA, FC	Mouse IgG

CAT	Product Name	Application	Type
NS-042CN-F(E)	Mouse Anti-IL17A Recombinant Antibody; Fab Fragment (NS-042CN-F(E))	ELISA, FC	Mouse Fab

CAT	Product Name	Application	Type
NS-042CN-S(P)	Mouse Anti-IL17A Recombinant Antibody; scFv Fragment (NS-042CN-S(P))	ELISA, FC	Mouse scFv

CAT	Product Name	Application	Type
AFC-TAB-458CQ	Afuco™ Anti-IL17A ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-458CQ)	ELISA, IHC, FC, IP, IF, Inhib	ADCC enhanced antibody

CAT	Product Name	Application	Type
AFC-TAB-069	Afuco™ Anti-IL17A ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-069)	FC, IP, ELISA, Neut, FuncS, IF	ADCC enhanced antibody

CAT	Product Name	Application	Type
AFC-TAB-H08	Afuco™ Anti-IL17A ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H08)	ELISA, FC, IP, FuncS, IF, Neut	ADCC enhanced antibody

CAT	Product Name	Application	Type
AFC-TAB-H57	Afuco™ Anti-IL17A ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H57)	ELISA, IP, FC, FuncS, Neut	ADCC enhanced antibody

CAT	Product Name	Application	Type
VS-0424-XY147	AbPlus™ Anti-IL17A Magnetic Beads (41809)	IP, Protein Purification

CAT	Product Name	Application	Type
VS-0225-XY130	CytoStream™ Mouse Anti-IL17A Recombinant Antibody (VS-0225-XY130)	FC	Mouse IgG1, kappa

CAT	Product Name	Application	Type
VS-0325-FY96	Human Anti-IL17A (clone Antibody 7) scFv-Fc Chimera	ELISA, Inhib	Human IgG1, scFv-Fc

CAT	Product Name	Application	Type
VS-0525-XY3489	Anti-IL17A Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0525-XY3490	Anti-Human IL17A Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0525-XY3491	Anti-Mouse IL17A Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
VS-0625-YC167	Recombinant Anti-IL17A Eliminating Antibody, pH-Sensitive (VS-0625-YC167)	Antigen-Sweeping In Vivo.	Rat IgG1 kappa

CAT	Product Name	Application	Type
VS-0825-YC175	SmartAb™ Recombinant Anti-IL17A pH-dependent Antibody (VS-0825-YC175)	IF, IHC, ELISA, FC	Human IgG1 kappa

Specific Inquiry

Size:

Conjugation:

Endotoxin:

Purity:

Formats:

Isotype Switching:

Fc Engineering:

Modalities:

Add to compare

Request
Custom Production

See Our Custom Production in Action

Figure 1 Anti-HBV Recombinant Antibody (TAB-147) in SDS-PAGE

Download the full case study

Popular Products

Figure 5 Anti-RSV glycoprotein F Recombinant Antibody (TAB-709) in ELISA

Anti-RSV glycoprotein F Recombinant Antibody (TAB-709) (CAT# TAB-709)
Application: IF, IP, Neut, FuncS, ELISA, FC, WB

Figure 3 Anti-ErbB2 Antibody (TAB-053) in Dot Blot

Anti-Human ERBB2 Recombinant Antibody (TAB-053) (CAT# TAB-053)
Application: FuncS, IF, Neut, ELISA, FC, IP, WB

Figure 2 Anti-CAIX/CA9 Antibody (TAB-707) in WB

Anti-CAIX/CA9 Recombinant Antibody (Girentuximab) (CAT# TAB-707)
Application: WB, FuncS, IF, Neut, ELISA, FC, IP

Figure 6 Anti-Human VEGF Recombinant Antibody (TAB-011) in ELISA

Anti-Human VEGF Recombinant Antibody (Bevacizumab) (CAT# TAB-011)
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC

Figure 2 Anti-Human SLAMF7 Recombinant Antibody (TAB-570CL) in ELISA

Human Anti-SLAMF7 Recombinant Antibody (TAB-570CL) (CAT# TAB-570CL)
Application: IF, IHC, Inhib

Figure 2 Anti-C3b Antibody (PABL-420) in HPLC

Human Anti-C3b Recombinant Antibody (clone S77) (CAT# PABL-420)
Application: FuncS, Inhib, IP, ELISA

Figure 5 Human Anti-IL13 Recombinant Antibody (clone CNTO607) (PABW-170) in SEC-HPLC

Human Anti-IL13 Recombinant Antibody (clone CNTO607) (CAT# PABW-170)
Application: ELISA, WB, Neut

Figure 2 Human TIM3 Recombinant Antibody (TAB-0366CL) in WB

Anti-Human TIM3 Recombinant Antibody (TSR-022) (CAT# TAB-0366CL)
Application: FC, FuncS

Figure 2 Anti-Human TREM1 Recombinant Antibody (TAB-167CQ) in SDS-PAGE

Human Anti-TREM1 Recombinant Antibody (TAB-167CQ) (CAT# TAB-167CQ)
Application: ELISA, FC, WB, Block

Figure 5 Mouse Anti-HIV-1 p24 Recombinant Antibody (MRO-928CQ) in ELISA

Mouse Anti-HIV-1 p24 Recombinant Antibody (MRO-928CQ) (CAT# MRO-928CQ)
Application: ELISA

Figure 5 Recombinant Mouse Anti-Integrin alpha 1 Antibody (PABX-121) in ELISA

Recombinant Mouse Anti-Integrin alpha 1 Antibody (AQC2) (CAT# PABX-121)
Application: WB, ELISA, Neut, FuncS

Figure 2 Human CRCP Recombinant Antibody (TAB-426CQ) in HPLC

Human Anti-CRCP Recombinant Antibody (TAB-426CQ) (CAT# TAB-426CQ)
Application: ELISA, IHC, FC, IP, IF, Inhib

Figure 4 Anti-Human KLRC1 Recombinant Antibody (TAB-437CQ, Fc Silenced) in ELISA

Anti-Human KLRC1 Recombinant Antibody (TAB-437CQ) (CAT# TAB-437CQ)
Application: ELISA, IHC, FC, IP, IF, FuncS

Figure 2 Recombinant Anti-FGFR3 Antibody (PRO-001) (HPAB-0160-YC) in ELISA

Human Anti-FGFR3 Recombinant Antibody (clone PRO-001) (CAT# HPAB-0160-YC)
Application: FC, FuncS

Figure 2 Recombinant Mouse Anti-HSPA1A Antibody (HPAB-0243-YC) in HPLC

Mouse Anti-HSPA1A Recombinant Antibody (HPAB-0243-YC) (CAT# HPAB-0243-YC)
Application: ELISA, FC

Figure 2 Human Anti-NOTCH4 Recombinant Antibody (HPAB-0068-CN) in HPLC

Human Anti-NOTCH4 Recombinant Antibody (HPAB-0068-CN) (CAT# HPAB-0068-CN)
Application: Neut, IF

Figure 2 Recombinant Human Anti-C. botulinum A str. Hall botA Antibody (S25 chimera) (EPAF-0119CQ) in WB

Recombinant Human Anti-C. botulinum A str. Hall botA Antibody (S25 chimera) (CAT# EPAF-0119CQ)
Application: ELISA, Neut

Figure 5 Anti-Human CDH17 Recombinant Antibody (HPAB-2061LY) in ELISA

Human Anti-CDH17 Recombinant Antibody (clone D2101) (CAT# HPAB-2061LY)
Application: ELISA

Figure 2 Mouse Anti-His Tag Recombinant Antibody; Fab Fragment (HPAB-0328-WJ-F(E)) in ELISA

Mouse Anti-His Tag Recombinant Antibody; Fab Fragment (HPAB-0328-WJ-F(E)) (CAT# HPAB-0328-WJ-F(E))
Application: ELISA, WB

Figure 2 Anti-SLC39A6 Antibody in ELISA.

Recombinant Mouse Anti-SLC39A6 Antibody (1.7A4) (CAT# MOB-382CQ)
Application: ELISA, WB

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.