Anti-Lewis Y Recombinant Antibody (MB311) (CAT#: TAB-0015CL)

The humanized mAb is specific for the tumor-associated carbohydrate antigen Lewis Y. It can induce ADCC and CDC reactivity against LeY positive tumor cells.


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FC

Figure 1 Binding activity MB314 and MB311.

Figure 1 Binding activity MB314 and MB311.

(A) Binding of MB311 and MB314 to the Lewis-Y positive tumor cell line SKBR-3 was determined by flow cytometry. Geometric Mean fluorescence intensity (MFI) was plotted vs. the logarithm of the antibody concentration and fitted using a sigmoidal four parameter fit using GraphPad Prism 4 software for calculation of EC50 values. Mean and SD of triplicates are shown. Binding of 100 μg/ml MB311 (dark gray line) or MB314 (black line) to purified human NK-cells expressing the FcγRIII variant V/F (B), to the FcγRIII variant F/F (C) and the FcγRIII variant V/V (D). Results obtained with medium only are shown as light gray lines.

Kircheis, R., Halanek, N., Koller, I., Jost, W., Schuster, M., Gorr, G., ... & Nechansky, A. (2012, July). Correlation of ADCC activity with cytokine release induced by the stably expressed, glyco-engineered humanized Lewis Y-specific monoclonal antibody MB314. In MAbs (Vol. 4, No. 4, pp. 532-541). Taylor & Francis.

FuncS

Figure 2 MB314 and MB311 mediated effector functions.

Figure 2 MB314 and MB311 mediated effector functions.

CDC reactivity against two Lewis Y positive tumor cell lines was tested using a FACS based approach. Tumor cells were incubated with different concentrations of antibodies in the presence of human complement for 1 h. As comple-ment source frozen human serum was used. Cytotoxicity against SKBR-3 cells (A) or OVCAR-3 cells (B) was quantified by FACSTM analysis using a live/dead cell stain. Gated 7-AAD+ population (dead cells) as percentage of the whole population are plotted vs. the logarithm of the antibody concentra-tion and fitted using a sigmoidal four parameter fit using GraphPad Prism 4 software. Mean and SD of triplicates are shown.

Kircheis, R., Halanek, N., Koller, I., Jost, W., Schuster, M., Gorr, G., ... & Nechansky, A. (2012, July). Correlation of ADCC activity with cytokine release induced by the stably expressed, glyco-engineered humanized Lewis Y-specific monoclonal antibody MB314. In MAbs (Vol. 4, No. 4, pp. 532-541). Taylor & Francis.

FC

Figure 3 Intracellular Expression of tumor necrosis factor (TNF) by CD14+ cells, but not by CD3+ or CD56+ cells.

Figure 3 Intracellular Expression of tumor necrosis factor (TNF) by CD14+ cells, but not by CD3+ or CD56+ cells.

SKBR-3 target cells and effector cells (PBMCs from a healthy volunteer donor) at an E:T ratio of 25:1 were incubated with 74 ng/ml of MB311 for 5 h. Cells were harvested, stained with FITC-mouse anti-CD14 antibody or mouse anti- CD56 antibody and anti-mIgG Dylight 488, or Cy5-mouse anti-CD3 antibody, fixed, permeabilized and subsequently stained with PE-mouse anti human TNFα antibody. Staining was measured by FACS analysis. The data reflect gating on PBMC effector cells (red) (and excluding target cells, green) based on forward and side scattered light signals (left part). Intracellular staining of TNFα gated on CD14+ or CD56+ (middle part) and CD3+ cells are shown (right part).

Kircheis, R., Halanek, N., Koller, I., Jost, W., Schuster, M., Gorr, G., ... & Nechansky, A. (2012, July). Correlation of ADCC activity with cytokine release induced by the stably expressed, glyco-engineered humanized Lewis Y-specific monoclonal antibody


Specifications

  • Host Species
  • Human
  • Type
  • Humanized Antibody
  • Clone
  • MB311
  • Applications
  • FC, FuncS
  • Related Disease
  • LeY expressing tumors

Applications

  • Application Notes
  • This antibody has been reported for use in Flow Cytometry and Function Assay.

Target

  • Alternative Names
  • Lewis Y; Lewis Y antigen; Lewis-Y; Lewis-Y antigen; Lewis(y) antigen; Lewis(y)

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "Clone MB311"

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Single-domain Antibody

CAT Product Name Application Type
NAB-1634-VHH Recombinant Anti-Human Lewis Y VHH Single Domain Antibody WB, IP, ChiP, Neut, ELISA Llama VHH

Humanized Antibody

CAT Product Name Application Type
TAB-730 Humanized Anti-Lewis Y Recombinant Antibody (clone CBR96) IP, IF, FuncS, FC, Neut, ELISA, ICC Humanized (from mouse) IgG1, κ
TAB-488CL Anti-Human Lewis Y Recombinant Antibody (MB 311) Cyt Antibody
TAB-0016CL Anti-Lewis Y Recombinant Antibody (Hu3s193) RIA, IHC Humanized Antibody
TAB-0017CL Anti-Lewis Y Recombinant Antibody (hABL364) CDC, ADCC, ELISA Humanized Antibody
PABX-003 Recombinant Human Anti-LewisY Antibody (hu3S193) WB, IF, FuncS, IHC, Inhib IgG

Human Antibody

CAT Product Name Application Type
PABX-003-S (P) Recombinant Human Anti-LewisY Antibody scFv Fragment (hu3S193) WB, IF, FuncS scFv

Recombinant Antibody

Fab Fragment Antibody

scFv Fragment Antibody

CAT Product Name Application Type
FAMAB-0207CQ-S(P) Mouse Anti-Lewis Y Recombinant Antibody (clone MSL5); scFv Fragment FC, IHC Mouse scFv

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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