Anti-Lewis Y Recombinant Antibody (Hu3s193) (CAT#: TAB-0016CL)
This antibody (clone Hu3s193) is a humanized mAb recognizing Lewis Y. Hu3s193 binds preferentially to the Lewis Y positive cells, not to normal tissue.
Specific Inquiry
RIA
Figure 1 Whole body scintigraphic images of radiolabeled 111In-CHX-A'-DTPA-hu3S193 in an athymic BALB/c nude mouse bearing s.c. MCF-7 (breast) tumor in the left flank (right side of image) and SW1222 colon tumor in the right flank (left side of image).
Dorsal images were taken at 10 min (A), 24 h (B), 72 h (C), and 120 h (D) after injection of radiolabeled hu3S193, with the mouse supine on the gamma camera. A standard of known activity was included in the field of view and is visible in the upper left quadrant of each image.
Clarke, K., Lee, F. T., Brechbiel, M. W., Smyth, F. E., Old, L. J., & Scott, A. M. (2000). In vivo biodistribution of a humanized anti-Lewis Y monoclonal antibody (hu3S193) in MCF-7 xenografted BALB/c nude mice. Cancer research, 60(17), 4804-4811.
RIA
Figure 2 The 125I/111In-hu3S193 biodistribution study.
Distribution of 125I/111In radiolabeled hu3S193 in an MCF-7 tumor xenograft 120 h after antibody injection as determined by autoradiography (A). Localization of radiolabel is compared with the location of viable tumor cells by H&E staining (B) and to antigen distribution as shown by immunohistochemistry (C). D is a control slide for antigen detection in which huA33 was applied as the primary antibody instead of hu3S193, and negligible staining was noted.
Clarke, K., Lee, F. T., Brechbiel, M. W., Smyth, F. E., Old, L. J., & Scott, A. M. (2000). In vivo biodistribution of a humanized anti-Lewis Y monoclonal antibody (hu3S193) in MCF-7 xenografted BALB/c nude mice. Cancer research, 60(17), 4804-4811.
IHC
Figure 3 Small-cell lung cancer tumors showing 1 (A) or 4 (B) immunohistochemical staining with mu3S193.
Immunohistochemical staining with mu3S193.
Krug, L. M., Milton, D. T., Jungbluth, A. A., Chen, L. C., Quaia, E., Pandit-Taskar, N., ... & Smith-Jones, P. (2007). Targeting Lewis Y (LeY) in small cell lung cancer with a humanized monoclonal antibody, hu3S193: a pilot trial testing two dose levels. Journal of Thoracic Oncology, 2(10), 947-952.
Specifications
- Host Species
- Human
- Type
- Humanized Antibody
- Clone
- Hu3s193
- Applications
- RIA, IHC
- Related Disease
- LeY expressing tumors
Applications
- Application Notes
- This antibody has been reported for use in Radioimmunoassay and Immunohistochemistry.
Radioimmunoassay: Immediately after sacrifice, an MCF-7 tumor from one mouse at time points 24, 48, 72, and 120 h in the 125I/111In biodistribution study was excised, frozen, and sectioned on a cryomicrotome. Sequential 5-μm sections were used for direct autoradiography and for H&E staining. Tissue sections were placed on silane-coated glass slides and allowed to dry. Glass slides were placed face down in contact with emulsion of Hyperfilm-MP film or standard radiography film. The glass slides and film were enclosed in a cassette and stored at −70°C. An image intensifier was used for Hyperfilm samples and was located directly behind and in contact with the film. Slides and film were taken up at varying time intervals, and film was developed in a standard automatic film processor.
Target
- Alternative Names
- Lewis Y; Lewis Y antigen; Lewis-Y; Lewis-Y antigen; Lewis(y) antigen; Lewis(y)
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1634-VHH | Recombinant Anti-Human Lewis Y VHH Single Domain Antibody | WB, IP, ChiP, Neut, ELISA | Llama VHH |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-730 | Humanized Anti-Lewis Y Recombinant Antibody (clone CBR96) | IP, IF, FuncS, FC, Neut, ELISA, ICC | Humanized (from mouse) IgG1, κ |
| TAB-488CL | Anti-Human Lewis Y Recombinant Antibody (MB 311) | Cyt | Antibody |
| TAB-0015CL | Anti-Lewis Y Recombinant Antibody (MB311) | FC, FuncS | Humanized Antibody |
| PABX-003 | Recombinant Human Anti-LewisY Antibody (hu3S193) | WB, IF, FuncS, IHC, Inhib | IgG |
| PABX-003-F (E) | Recombinant Human Anti-LewisY Antibody Fab Fragment (hu3S193) | WB, IF, FuncS | Fab |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0018CL | Anti-Lewis Y Recombinant Antibody (BR55-2) | Activ, Inhib | |
| TAB-0018CL-S(P) | Anti-Lewis Y Recombinant Antibody scFv Fragment (BR55-2) | FuncS, Inhib | |
| TAB-0018CL-F(E) | Anti-Lewis Y Recombinant Antibody Fab Fragment (BR55-2) | FuncS, Inhib | |
| PABX-131 | Recombinant Mouse Anti-LewisY Antibody (MBR96) | WB, Neut, FuncS | IgG |
| PABX-131-F (E) | Recombinant Mouse Anti-LewisY Antibody Fab Fragment (MBR96) | WB, Neut, FuncS | Fab |
Fc Glycosylation
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-044LC | Recombinant Anti-Human Lewis Y Antibody (Fc glycosylation) | ELISA | Humanized antibody |
| Gly-045LC | Recombinant Anti-Human Lewis Y Antibody (Fc glycosylation) | ELISA | Humanized antibody |
| Gly-103LC | Recombinant Anti-Human Lewis Y Antibody (Fc glycosylation) | ELISA | Humanized antibody |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-003-S (P) | Recombinant Human Anti-LewisY Antibody scFv Fragment (hu3S193) | WB, IF, FuncS | scFv |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0207CQ | Mouse Anti-Lewis Y Recombinant Antibody (clone MSL5) | ELISA, FC, IHC | Mouse IgG |
| FAMAB-1327CQ | Human Anti-Lewis Y Recombinant Antibody (clone BR96) | IHC, Cyt | Human IgG1 |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0207CQ-F(E) | Mouse Anti-Lewis Y Recombinant Antibody (clone MSL5); Fab Fragment | FC, IHC | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0207CQ-S(P) | Mouse Anti-Lewis Y Recombinant Antibody (clone MSL5); scFv Fragment | FC, IHC | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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