Recombinant Mouse Antibody clone MSL5, which is specific to LewisY. This antibody is against the tumor-associated carbohydrate Ag Lewis Y (Fuc alpha 1-->2Gal beta 1-->4[Fuc alpha 1-->3] GlcNAc beta 1-->3R).
Figure 2 ELISA
A, Functional ELISA of mAbs MSL5 IgM (circles) and HI 8A lgG3 (squares) with Ley-BSA (white symbols) and Lex-BSA Ag (black symbols). The addition of 1 mM of LeY Ag to either H18A and MSL5 (10-40 pg/ml each) resulted in more than 95% reduction of the binding signal to Ley-BSA in each case. Binding of the Abs to milk powder, which was used as the blocking agent, was not detectable (inhibition and milk-powder data not shown). B, Functional ELISA of the tetrameric anti-LeY mini-antibody MSLS-p53his (circles) and dimeric anti-LeY mini-antibody MSL5-dHLXhis (squares) with LeYBSA (white symbols) and Lex-BSA Ag (black symbols). The addition of 1 mM of LeY Ag to both mini-antibody constructs (100-330 p.g/nil each) resulted in more than 95% reduction of the binding signal to Ley-BSA in each case. Binding of the Abs to milk powder, which was used as the blocking agent, was not detectable (inhibition and milkpowder data not shown). Bound Abs were detected with either IgM, IgG, or FLAG-specific alkaline phosphatase conjugates.
Rheinnecker, M., Hardt, C., Ilag, L. L., Kufer, P., Gruber, R., Hoess, A., ... & Pack, P. (1996). Multivalent antibody fragments with high functional affinity for a tumor-associated carbohydrate antigen. The Journal of Immunology, 157(7), 2989-2997.
Figure 3 Analysis of Ley-BSA-binding behavior of the MSL5-scFv (A), dimeric mini-antibody MSL5-dHLXhis (B), tetrameric mini-antibody MSL5-p53his (C), and MSL5-lgM (D) with surface plasmon resonance on a BlAcore biosensor instrument.
Shown are overlay plots of each construct at various concentrations (MSL5-scFv, 0.37-3.75 μM; dimeric mini-antibody MSL5-dHLXhis, 0.1 2-2.4 μM; tetrameric miniantibody MSL5-p53his, 0.08-1.07 μM; and IgM, 0.3-14 nM).
Rheinnecker, M., Hardt, C., Ilag, L. L., Kufer, P., Gruber, R., Hoess, A., ... & Pack, P. (1996). Multivalent antibody fragments with high functional affinity for a tumor-associated carbohydrate antigen. The Journal of Immunology, 157(7), 2989-2997.
Figure 4 Elution of MSL5-lgM (14 nM) from Ley-BSA using soluble LeY Ag.
The injection of 1 mM of LeY Ag is indicated by the arrow
Rheinnecker, M., Hardt, C., Ilag, L. L., Kufer, P., Gruber, R., Hoess, A., ... & Pack, P. (1996). Multivalent antibody fragments with high functional affinity for a tumor-associated carbohydrate antigen. The Journal of Immunology, 157(7), 2989-2997.
Figure 5 Overlay plot of individual dissociation curves of the MSL5-scFv fragment (3.5 μM) (A), dimeric mini-antibody MSL5-dHLXhis (2.4 μM) (B), tetrameric mini-antibody MSL5-p53his (1.07 μM) (C), and the MSL5-lgM (14 nM) (D).
Rheinnecker, M., Hardt, C., Ilag, L. L., Kufer, P., Gruber, R., Hoess, A., ... & Pack, P. (1996). Multivalent antibody fragments with high functional affinity for a tumor-associated carbohydrate antigen. The Journal of Immunology, 157(7), 2989-2997.
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• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
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CAT | Product Name | Application | Type |
---|---|---|---|
NAB-1634-VHH | Recombinant Anti-Human Lewis Y VHH Single Domain Antibody | WB, IP, ChiP, Neut, ELISA | Llama VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-488CL | Anti-Human Lewis Y Recombinant Antibody (MB 311) | Cyt | Antibody |
TAB-0015CL | Anti-Lewis Y Recombinant Antibody (MB311) | FC, FuncS | Humanized Antibody |
TAB-0016CL | Anti-Lewis Y Recombinant Antibody (Hu3s193) | RIA, IHC | Humanized Antibody |
TAB-0017CL | Anti-Lewis Y Recombinant Antibody (hABL364) | CDC, ADCC, ELISA | Humanized Antibody |
PABX-003 | Recombinant Human Anti-LewisY Antibody (hu3S193) | WB, IF, FuncS, IHC, Inhib | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-0018CL | Anti-Lewis Y Recombinant Antibody (BR55-2) | Activ, Inhib | |
TAB-0018CL-S(P) | Anti-Lewis Y Recombinant Antibody scFv Fragment (BR55-2) | FuncS, Inhib | |
TAB-0018CL-F(E) | Anti-Lewis Y Recombinant Antibody Fab Fragment (BR55-2) | FuncS, Inhib | |
TAB-0650CL-F(E) | Anti-Human Lewis Y Recombinant Antibody Fab Fragment (3S193) | ELISA | |
PABX-131-F (E) | Recombinant Mouse Anti-LewisY Antibody Fab Fragment (MBR96) | WB, Neut, FuncS | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-044LC | Recombinant Anti-Human Lewis Y Antibody (Fc glycosylation) | ELISA | Humanized antibody |
Gly-045LC | Recombinant Anti-Human Lewis Y Antibody (Fc glycosylation) | ELISA | Humanized antibody |
Gly-103LC | Recombinant Anti-Human Lewis Y Antibody (Fc glycosylation) | ELISA | Humanized antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
PABX-003-S (P) | Recombinant Human Anti-LewisY Antibody scFv Fragment (hu3S193) | WB, IF, FuncS | scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0207CQ-F(E) | Mouse Anti-Lewis Y Recombinant Antibody (clone MSL5); Fab Fragment | FC, IHC | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0207CQ-S(P) | Mouse Anti-Lewis Y Recombinant Antibody (clone MSL5); scFv Fragment | FC, IHC | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-1327CQ | Human Anti-Lewis Y Recombinant Antibody (clone BR96) | IHC, Cyt | Human IgG1 |
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