Human Anti-PCSK9 Recombinant Antibody (clone J16)
CAT#: PABC-196
Recombinant Human Antibody (J16) is capable of binding to PCSK9, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-PCSK9 mAb and CH1-3 region of human IgG and a light chain (LC) encoding VL from anti-PCSK9 proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This antibody, J16, potently inhibit PCSK9 binding to the LDLR extracellular domain and PCSK9-mediated down-regulation of LDLR in vitro.
Published Data
Gene Expression
IF
Figure 1 PCSK9 internalization with J16.
PCSK9-488 internalization in the presence of 5F6 and J16 in HepG2 cells. Internalized human and mouse antibodies shown in blue and red, respectively. Scale bars, 10 μm.
DeVay, R. M., Yamamoto, L., Shelton, D. L., & Liang, H. (2015). Common proprotein convertase subtilisin/kexin type 9 (PCSK9) epitopes mediate multiple routes for internalization and function. PloS one, 10(4).
IP
Figure 2 APLP2 and LDLR interactions with PCSK9 and their regulation of PCSK9 function.
Western blot showing APLP2, PCSK9, orTransferrin receptor (TFNR) levels in input fraction (I), IC or J16 immunoprecipitated samples (IP Ab.) in the absence or presence of 5F6 Fab or 12E3 Fab.
DeVay, R. M., Yamamoto, L., Shelton, D. L., & Liang, H. (2015). Common proprotein convertase subtilisin/kexin type 9 (PCSK9) epitopes mediate multiple routes for internalization and function. PloS one, 10(4).
IF
Figure 3 Characterization of PCSK9 internalization and subsequent J16 or LDLR lysosomal trafficking in HepG2 cells.
Colocalization of LDLR (green) with lysosomes (Lamp2; red) in DAPI (blue)-stained cells in the presence of PCSK9IC or PCSK9J16. Scale bars, 10 μm.
DeVay, R. M., Shelton, D. L., & Liang, H. (2013). Characterization of proprotein convertase subtilisin/kexin type 9 (PCSK9) trafficking reveals a novel lysosomal targeting mechanism via amyloid precursor-like protein 2 (APLP2). Journal of Biological Chemistry, 288(15), 10805-10818.
WB
Figure 4 Identification and biochemical characterization of interactions between PCSK9 and members of the amyloid precursor protein family.
Western blots showing APLP2, APP, PCSK9-His, or TFNR levels in input (I), IC IP, or J16 IP samples. co-IPs were performed at pH 6.0 using HepG2 lysates.
DeVay, R. M., Shelton, D. L., & Liang, H. (2013). Characterization of proprotein convertase subtilisin/kexin type 9 (PCSK9) trafficking reveals a novel lysosomal targeting mechanism via amyloid precursor-like protein 2 (APLP2). Journal of Biological Chemistry, 288(15), 10805-10818.
ELISA
Figure 5 Recombinant PCSK9, APLP2, and LDLR form a trimeric complex in vitro.
ELISA of APLP2-PCSK9-LDLR complex with 4 μg/ml PCSK9 and 12 μg/ml IC, J16, or 5F6.
DeVay, R. M., Shelton, D. L., & Liang, H. (2013). Characterization of proprotein convertase subtilisin/kexin type 9 (PCSK9) trafficking reveals a novel lysosomal targeting mechanism via amyloid precursor-like protein 2 (APLP2). Journal of Biological Chemistry, 288(15), 10805-10818.
❮
❯
RNA Expression
Figure 1 RNA cell line category: Cell line enhanced (A-431, CACO-2, HaCaT, HeLa, RPTEC TERT1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000169174-PCSK9
❮
❯
Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human PCSK9
- Species Reactivity
- Human
- Clone
- J16
- Applications
- IF, IP, WB, ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western Blot, Immunoprecipitation, Immunofluorescence and Enzyme-Linked Immunosorbent Assay.
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for PABC-196. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Clone J16"
- CAT
- Product Name
See other products for "PCSK9"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-H31 | Human Anti-PCSK9 Recombinant Antibody (TAB-H31) | FC, IP, ELISA, Neut, FuncS, IF, IHC | Human IgG2, λ |
| TAB-0953CL-S(P) | Human Anti-PCSK9 Recombinant Antibody; scFv Fragment (TAB-0953CL-S(P)) | ELISA | Human scFv |
| TAB-0954CL-S(P) | Human Anti-PCSK9 Recombinant Antibody; scFv Fragment (TAB-0954CL-S(P)) | WB | Human scFv |
| TAB-0955CL-S(P) | Mouse Anti-PCSK9 Recombinant Antibody; scFv Fragment (TAB-0955CL-S(P)) | WB | Mouse scFv |
| TAB-0959CL-S(P) | Human Anti-PCSK9 Recombinant Antibody; scFv Fragment (TAB-0959CL-S(P)) | ELISA, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABZ-101 | Human Anti-PCSK9 Recombinant Antibody (clone 1D05) | ELISA, WB, Neut | Human IgG2 |
| HPAB-0234-YC | Mouse Anti-PCSK9 Recombinant Antibody (HPAB-0234-YC) | ELISA, FuncS | Mouse IgG |
| HPAB-0041-WJ | Mouse Anti-PCSK9 Recombinant Antibody (clone Ab-3D5) | ELISA, WB, Inhib | Mouse IgG, κ |
| HPAB-667-FY | Human Anti-PCSK9 Recombinant Antibody (HPAB-667-FY) | Inhib, SPR, ELISA | Human IgG2 |
| HPAB-668-FY | Human Anti-PCSK9 Recombinant Antibody (HPAB-668-FY) | Inhib, SPR | Human IgG2 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-670 | Human Anti-PCSK9 Recombinant Antibody; Fab Fragment (PFBL-670) | WB, FuncS | Human Fab |
| PFBL-671 | Mouse Anti-PCSK9 Recombinant Antibody (clone mAb J16); Fab Fragment | WB, ELISA, FuncS | Mouse Fab |
| PFBL-672 | Human Anti-PCSK9 Recombinant Antibody (clone CBL36Q); Fab Fragment | ELISA, WB | Human Fab |
| HPAB-0041-WJ-F(E) | Mouse Anti-PCSK9 Recombinant Antibody (clone Ab-3D5); Fab Fragment | ELISA, WB | Mouse Fab |
| HPAB-1439LY-F(E) | Human Anti-PCSK9 Recombinant Antibody (clone PA4); Fab Fragment | ELISA, Block | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0020CL | Anti-Human PCSK9 Recombinant Antibody (LPD1282/LPD1462 {i.e., antibody 1 F11)) | LDL-c uptake assay | Humanized Antibody |
| TAB-0020CL-S(P) | Anti-Human PCSK9 Recombinant Antibody scFv Fragment (LPD1282/LPD1462 {i.e., antibody 1 F11)) | LDL-c uptake assay | Humanized Antibody |
| TAB-0957CL-S(P) | Anti-Human PCSK9 Recombinant Antibody scFv Fragment (h7F8) | ELISA | Humanized antibody |
| TAB-0958CL-S(P) | Anti-Human PCSK9 Recombinant Antibody scFv Fragment (h7F8ml2) | ELISA | Humanized antibody |
| TAB-0020CL-F(E) | Anti-Human PCSK9 Recombinant Antibody Fab Fragment (LPD1282/LPD1462 {i.e., antibody 1 F11)) | LDL-c uptake assay | Humanized Antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0956CL | Anti-Human PCSK9 Recombinant Antibody (m7F8) | ELISA | |
| TAB-0956CL-S(P) | Anti-Human PCSK9 Recombinant Antibody scFv Fragment (m7F8) | ELISA | |
| TAB-0960CL-S(P) | Anti-Human PCSK9 Recombinant Antibody scFv Fragment (Ab1) | ELISA | Rabbit mAb |
| TAB-0956CL-F(E) | Anti-Human PCSK9 Recombinant Antibody Fab Fragment (m7F8) | ELISA | |
| TAB-0960CL-F(E) | Anti-Human PCSK9 Recombinant Antibody Fab Fragment (Ab1) | ELISA | Rabbit mAb |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0080CL-VHH | Anti-Human PCSK9 VHH Single Domain Antibody (TAB-0080CL-VHH) | FuncS, Inhib, FC, WB | Single domain antibody |
| TAB-0081CL-VHH | Anti-Human PCSK9 VHH Single Domain Antibody (TAB-0081CL-VHH) | FuncS, Inhib, FC, WB | Single domain antibody |
| TAB-0082CL-VHH | Anti-Human PCSK9 VHH Single Domain Antibody (TAB-0082CL-VHH) | FuncS, Inhib, FC, WB | Single domain antibody |
| TAB-0083CL-VHH | Anti-Human PCSK9 VHH Single Domain Antibody (TAB-0083CL-VHH) | FuncS, Inhib, FC, WB | Single domain antibody |
| TAB-0084CL-VHH | Anti-Human PCSK9 VHH Single Domain Antibody (TAB-0084CL-VHH) | FuncS, Inhib, FC, WB | Single domain antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1826CQ | Recombinant Human Anti-PCSK9 Antibody, Unconjugated | Neut | IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2618 | Hi-Affi™ Recombinant Rabbit Anti-PCSK9 Monoclonal Antibody (DS2618AB) | WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-N0377-YC-S(P) | Human Anti-PCSK9 Recombinant Antibody; scFv Fragment (HPAB-N0377-YC-S(P)) | FC, FuncS | Human scFv |
| HPAB-0318-YJ-S(P) | Human Anti-PCSK9 Recombinant Antibody; scFv Fragment (HPAB-0318-YJ-S(P)) | Antagonist, ELISA, FuncS | Human scFv |
| HPAB-1373-FY-F(E) | Human Anti-PCSK9 Recombinant Antibody; Fab Fragment (HPAB-1373-FY-F(E)) | ELISA | Human Fab |
| HPAB-1374-FY-F(E) | Human Anti-PCSK9 Recombinant Antibody; Fab Fragment (HPAB-1374-FY-F(E)) | ELISA | Human Fab |
| HPAB-1375-FY-F(E) | Human Anti-PCSK9 Recombinant Antibody; Fab Fragment (HPAB-1375-FY-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-758 | Afuco™ Anti-PCSK9 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-758) | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
| AFC-TAB-H10 | Afuco™ Anti-PCSK9 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H10) | FC, IP, ELISA, Neut, FuncS | ADCC enhanced antibody |
| AFC-TAB-H59 | Afuco™ Anti-PCSK9 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H59) | Neut, ELISA, IF, IP, FuncS, FC | ADCC enhanced antibody |
| AFC-TAB-H31 | Afuco™ Anti-PCSK9 Recombinant Antibody (AFC-TAB-H31), ADCC Enhanced | FC, IP, ELISA, Neut, FuncS, IF | Human IgG1, λ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY208 | AbPlus™ Anti-PCSK9 Magnetic Beads (VS-0424-XY208) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY208 | Human Anti-PCSK9 scFv-Fc Chimera (VS-0325-FY208) | WB, Block | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5250 | Anti-PCSK9 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC142 | Recombinant Anti-PCSK9 (AA 31-152 x AA 213-219) Biparatopic Antibody, Tandem scFv (Clone 6E2 x Clone 3D5) | ELISA | Tandem scFv |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
-YJ-3.jpg)
Application: ELISA
Application: IHC, ELISA, FC, WB, ADCC, FuncS
Application: Neut, Inhib, FC, ELISA
Application: FC, FRET, Internalization
Application: WB, ELISA, FuncS, Inhib, PK, IP, SPR
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, FuncS
-2.png)
Application: ELISA, WB
-2.png)
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
