Recombinant Anti-human PRNP Intrabody [+36 GFP] (CAT#: IAB-B034(G))
Recombinant anti-PRNP intrabody in fusion with +36GFP works within cells to bind PRNP, expressed in CHO cells. Anti-prion intrabodies targeted to the lumen of the endoplasmic reticulum provide a simple and effective means to inhibit the transport of PrPC to the cell surface.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/if_selected.jpg
Figure 2 IHC staining of human cerebellum
(Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/ihc_selected.jpg
(Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/ihc_selected.jpg
Figure 3 Cerebral cortex
(Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42754/95550_B_7_5.jpg
(Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42754/95550_B_7_5.jpg
Figure 4 Caudate
(Neuronal cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/95554_B_9_7.jpg
(Neuronal cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/95554_B_9_7.jpg
Figure 5 Colon
(Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42754/95550_A_9_3.jpg
(Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42754/95550_A_9_3.jpg
Figure 6 RNA cell line category: Cell line enhanced (WM-115)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000171867-PRNP
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000171867-PRNP
Figure 6 RNA cell line category: Cell line enhanced (WM-115)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000171867-PRNP
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000171867-PRNP
Specifications
- Immunogen
- human prion protein
- Host Species
- mouse
- Type
- scFv-(+36GFP)
- Cell Penetrating Peptide
- +36 GFP
GFP with a theoretical net charge of +36 (+36 GFP) penetrated several different mammalian cell lines at very low (nanomolar) concentrations. Cationic peptides and proteins have been observed to penetrate cells, and both natural and synthetic cell-penetrating peptides are widely used as delivery vehicles. However, whereas a covalent link between the cell-penetrating peptide and its cargo is typically required.
- Species Reactivity
- human
- Applications
- Formats of immunological tests: Western blot; Coimmunopricipation; Functional studies
Product Property
- Purity
- >95% as judged by SDS-polyacrylamide gel electrophoresis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Target
- Alternative Names
- PRNP; prion protein; CJD; GSS; PrP; ASCR; KURU; PRIP; PrPc; CD230; AltPrP; p27-30; PrP27-30; PrP33-35C; major prion protein; CD230 antigen; prion-related protein
- Gene ID
- 5621
- UniProt ID
- P04156
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "PRNP"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1227z | Mouse Anti-PRNP Recombinant Antibody (clone 25G7) | WB, IP, IF, IHC, FC, ELISA | Mouse IgG1, κ |
| PABW-089 | Mouse Anti-PRNP Recombinant Antibody (clone 3F4) | ELISA | Mouse IgG2a |
| PABC-107 | Mouse Anti-PRNP Recombinant Antibody (clone ICSM 18) | ELISA, Inhib, IF | Mouse IgG |
| PABW-107 | Mouse Anti-PRNP Recombinant Antibody (clone VRQ14) | ELISA | Mouse IgG2a |
| FAMAB-0273CQ | Mouse Anti-PRNP Recombinant Antibody (clone mAb 106) | ELISA, WB, IHC | Mouse IgG2b, κ |
Intrabody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B034(A) | Recombinant Anti-human PRNP Intrabody [(D-Arg)9] | IF, FC, WB, FuncS | scFv-(D-Arg)9 |
| IAB-B034(T) | Recombinant Anti-human PRNP Intrabody [Tat] | IF, ELISA, FuncS | scFv-Tat |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PNBL-026 | Recombinant Anti-Human PRNP VHH Single Domain Antibody (Nb484) | WB, ELISA, FuncS | Llama VHH |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-942LC | Anti-Sheep PRNP Recombinant Antibody (6.1) | ELISA | Mouse antibody |
| MRO-948LC | Anti-Mouse Prnp Recombinant Antibody (6H10) | ELISA | Mouse antibody |
| MRO-949LC | Anti-Mouse Prnp Recombinant Antibody (31C6) | ELISA | Mouse antibody |
| MRO-950LC | Anti-Mouse Prnp Recombinant Antibody (72-5) | ELISA | Mouse antibody |
| MRO-951LC | Anti-Mouse Prnp Recombinant Antibody (44B1) | ELISA | Mouse antibody |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-943LC | Anti-Mouse Prnp Recombinant Antibody (D13) | ELISA | Human antibody |
| MRO-944LC | Anti-Mouse Prnp Recombinant Antibody (D18) | ELISA | Human antibody |
| MRO-945LC | Anti-Mouse Prnp Recombinant Antibody (R72) | ELISA | Human antibody |
| MRO-946LC | Anti-Mouse Prnp Recombinant Antibody (R1) | ELISA | Human antibody |
| MRO-947LC | Anti-Mouse Prnp Recombinant Antibody (R2) | ELISA | Human antibody |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0727MZ | Chicken Anti-PRNP Polyclonal IgY | ICC/IF | Chicken antibody |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2871 | Hi-Affi™ Recombinant Rabbit Anti-PRNP Monoclonal Antibody (DS2871AB) | WB, FC, IHC-P, ICC/IF | IgG |
Epitope-Specific Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-0136LC | Recombinant Mouse Anti-Sheep Prnp Antibody (VRQ14) | ELISA | IgG2a |
| EPAF-0984LC | Recombinant Mouse Anti-Human PRNP Antibody (mab 14D3) | ELISA | IgG |
| EPAF-1254LC | Recombinant Mouse Anti-PRNP Antibody (V5B2) | WB | |
| EPAF-1255LC | Recombinant Mouse Anti-PRNP Antibody (27-2) | WB | IgG1 |
| EPAF-1256LC | Recombinant Mouse Anti-PRNP Antibody (7D4) | WB | IgG1 |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0272CQ-F(E) | Human Anti-PRNP Recombinant Antibody (clone ch31C6); Fab Fragment | ELISA, WB, IHC | Chimeric (mouse/human) Fab |
| FAMAB-0273CQ-F(E) | Mouse Anti-PRNP Recombinant Antibody (clone mAb 106); Fab Fragment | ELISA, WB, IHC | Mouse Fab |
| FAMAB-0274CQ-F(E) | Mouse Anti-PRNP Recombinant Antibody (clone mAb 110); Fab Fragment | ELISA, WB, IHC | Mouse Fab |
| FAMAB-0275CQ-F(E) | Mouse Anti-PRNP Recombinant Antibody (clone mAb 132); Fab Fragment | ELISA, WB, IHC | Mouse Fab |
| FAMAB-0294-CN-F(E) | Human Anti-PRNP Recombinant Antibody (clone D18); Fab Fragment | WB, IF, IHC | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0050-CN-S(P) | Chicken Anti-PRNP Recombinant Antibody (clone phAb4-31); scFv Fragment | ELISA | Chicken scFv |
| HPAB-0282CQ-S(P) | Human Anti-PRNP Recombinant Antibody (clone boPrP-15); scFv Fragment | ELISA, WB | Human scFv |
| HPAB-0283CQ-S(P) | Human Anti-PRNP Recombinant Antibody (clone huPrP-18); scFv Fragment | ELISA, WB | Human scFv |
| HPAB-0284CQ-S(P) | Human Anti-PRNP Recombinant Antibody (clone huPrP-39); scFv Fragment | ELISA, WB | Human scFv |
| HPAB-0285CQ-S(P) | Human Anti-PRNP Recombinant Antibody (clone boPrP-20); scFv Fragment | ELISA, WB | Human scFv |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for IAB-B034(G). Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.