Mouse Anti-PRNP Recombinant Antibody (clone ICSM 18) (CAT#: PABC-107)

Figure 1 Inhibition of PrPSc formation in ScRov cells induced by antibodies raised against alpha- and beta-PrP (ICSM mAbs).

a, dose-dependent inhibition of PrPSc accumulation in ScRov cells induced by the ICSM mAbs. PrPSc Levels were analyzed by immunoblot after 3 weeksof treatment with the mAbs or DS500. Controls were left untreated. b,time course of ICSM mAb-mediated clearance of PrPSc. Cells weretreated in triplicate for 6 weeks at the dose of 1 g/ml or were leftuntreated. Measurements were performed in triplicate, and valuesrepresent its mean S.D. The treatment was lifted after 6 weeks tostudy the re-emergence of PrPSc. c, the rapidity of PrPSc reappearanceis a function of the treatment performed. Levels of the protein wereanalyzed by immunoblot after 6 weeks of treatment with ICSM 35 andICSM 19 and DS500. The treatment was then lifted, and PrPSc production was assessed at several time points. This was compared withuntreated cells

Beringue V , Vilette D , Mallinson G , et al. PrPSc Binding Antibodies Are Potent Inhibitors of Prion Replication in Cell Lines[J]. Journal of Biological Chemistry, 2004, 279(38):39671-39676.

Figure 2 Reactivity of the ICSM mAbs for Rov cells PrPC and PrPSc.

a, the ICSM mAb immunoreactivity for native PrPC and protease-resistant PrPSc (PrP27–30) was assessed by immunoprecipitation from cell lysates of Rov and ScRov cells, respectively. Controls (no mAb) omitting the precipitating mAb or using an isotypic mAb were negative. b and c, immunoreactivity for endogenous PrPC was assessed by immunoprecipitation on live cells. Controls using an isotypic mAb (no mAb) were negative. The amount immunoprecipitated by the mAbs were compared with the levels of PrPC present in the culture (Total PrPC). Values represent the mean  S.D. of three independent experiments. d, the binding abilities of the mAbs also were tested by immunofluorescence on live Rov and ScRov cells. In the ScRov cells, only ICSM 35 and 37 bound PrPSc, as detected by a dotlike intracellular staining (indicated by arrows). (×40).

Beringue V , Vilette D , Mallinson G , et al. PrPSc Binding Antibodies Are Potent Inhibitors of Prion Replication in Cell Lines[J]. Journal of Biological Chemistry, 2004, 279(38):39671-39676.