Recombinant Mouse Antibody (ICSM 18) is capable of binding to PRNP. ICSM 18 was an inhibitor of prion propagation (IC50 0. 7 nM) and in its affinity for PrPC (Kd 0. 1 nM).
Figure 1 Inhibition of PrPSc formation in ScRov cells induced by antibodies raised against alpha- and beta-PrP (ICSM mAbs).
a, dose-dependent inhibition of PrPSc accumulation in ScRov cells induced by the ICSM mAbs. PrPSc Levels were analyzed by immunoblot after 3 weeksof treatment with the mAbs or DS500. Controls were left untreated. b,time course of ICSM mAb-mediated clearance of PrPSc. Cells weretreated in triplicate for 6 weeks at the dose of 1 g/ml or were leftuntreated. Measurements were performed in triplicate, and valuesrepresent its mean S.D. The treatment was lifted after 6 weeks tostudy the re-emergence of PrPSc. c, the rapidity of PrPSc reappearanceis a function of the treatment performed. Levels of the protein wereanalyzed by immunoblot after 6 weeks of treatment with ICSM 35 andICSM 19 and DS500. The treatment was then lifted, and PrPSc production was assessed at several time points. This was compared withuntreated cells
Beringue V , Vilette D , Mallinson G , et al. PrPSc Binding Antibodies Are Potent Inhibitors of Prion Replication in Cell Lines[J]. Journal of Biological Chemistry, 2004, 279(38):39671-39676.
Figure 2 Reactivity of the ICSM mAbs for Rov cells PrPC and PrPSc.
a, the ICSM mAb immunoreactivity for native PrPC and protease-resistant PrPSc (PrP27–30) was assessed by immunoprecipitation from cell lysates of Rov and ScRov cells, respectively. Controls (no mAb) omitting the precipitating mAb or using an isotypic mAb were negative. b and c, immunoreactivity for endogenous PrPC was assessed by immunoprecipitation on live cells. Controls using an isotypic mAb (no mAb) were negative. The amount immunoprecipitated by the mAbs were compared with the levels of PrPC present in the culture (Total PrPC). Values represent the mean S.D. of three independent experiments. d, the binding abilities of the mAbs also were tested by immunofluorescence on live Rov and ScRov cells. In the ScRov cells, only ICSM 35 and 37 bound PrPSc, as detected by a dotlike intracellular staining (indicated by arrows). (×40).
Beringue V , Vilette D , Mallinson G , et al. PrPSc Binding Antibodies Are Potent Inhibitors of Prion Replication in Cell Lines[J]. Journal of Biological Chemistry, 2004, 279(38):39671-39676.
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1227z | Mouse Anti-PRNP Recombinant Antibody (clone 25G7) | WB, IP, IF, IHC, FC, ELISA | Mouse IgG1, κ |
MOB-1629CT | Recombinant Mouse anti-Human PRNP Monoclonal antibody (6C0) | ELISA, WB | |
FAMAB-0294-CN | Human Anti-PRNP Recombinant Antibody (clone D18) | WB, IF, IHC | Human IgG |
FAMAB-0295-CN | Human Anti-PRNP Recombinant Antibody (clone 3:3) | WB, IF, IHC | Human IgG |
FAMAB-0296-CN | Human Anti-PRNP Recombinant Antibody (clone 6:4) | WB, IF, IHC | Human IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
IAB-B034(A) | Recombinant Anti-human PRNP Intrabody [(D-Arg)9] | IF, FC, WB, FuncS | scFv-(D-Arg)9 |
IAB-B034(G) | Recombinant Anti-human PRNP Intrabody [+36 GFP] | WB, CO-IP, FuncS | scFv-(+36GFP) |
IAB-B034(T) | Recombinant Anti-human PRNP Intrabody [Tat] | IF, ELISA, FuncS | scFv-Tat |
CAT | Product Name | Application | Type |
---|---|---|---|
PNBL-026 | Recombinant Anti-Human PRNP VHH Single Domain Antibody (Nb484) | WB, ELISA, FuncS | Llama VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBW-089 | Mouse Anti-PRNP Recombinant Antibody (clone 3F4); scFv Fragment | ELISA | Mouse scFv |
PSBC-107 | Mouse Anti-PRNP Recombinant Antibody (clone ICSM 18); scFv Fragment | ELISA | Mouse scFv |
PSBW-107 | Mouse Anti-PRNP Recombinant Antibody (clone VRQ14); scFv Fragment | ELISA | Mouse scFv |
FAMAB-0054-CN-S(P) | Human Anti-PRNP Recombinant Antibody (clone hu4.44); scFv Fragment | ELISA | Human scFv |
FAMAB-0055-CN-S(P) | Human Anti-PRNP Recombinant Antibody (clone hu4.29); scFv Fragment | ELISA | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-942LC-S(P) | Anti-Sheep PRNP Recombinant Antibody scFv Fragment (6.1) | ELISA | Mouse antibody |
MRO-948LC-S(P) | Anti-Mouse Prnp Recombinant Antibody scFv Fragment (6H10) | ELISA | Mouse antibody |
MRO-949LC-S(P) | Anti-Mouse Prnp Recombinant Antibody scFv Fragment (31C6) | ELISA | Mouse antibody |
MRO-950LC-S(P) | Anti-Mouse Prnp Recombinant Antibody scFv Fragment (72-5) | ELISA | Mouse antibody |
MRO-951LC-S(P) | Anti-Mouse Prnp Recombinant Antibody scFv Fragment (44B1) | ELISA | Mouse antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-943LC-S(P) | Anti-Mouse Prnp Recombinant Antibody scFv Fragment (D13) | ELISA | Human antibody |
MRO-944LC-S(P) | Anti-Mouse Prnp Recombinant Antibody scFv Fragment (D18) | ELISA | Human antibody |
MRO-945LC-S(P) | Anti-Mouse Prnp Recombinant Antibody scFv Fragment (R72) | ELISA | Human antibody |
MRO-946LC-S(P) | Anti-Mouse Prnp Recombinant Antibody scFv Fragment (R1) | ELISA | Human antibody |
MRO-947LC-S(P) | Anti-Mouse Prnp Recombinant Antibody scFv Fragment (R2) | ELISA | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
BRD-0727MZ | Chicken Anti-PRNP Polyclonal IgY | ICC/IF | Chicken antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-2871 | Hi-Affi™ Recombinant Rabbit Anti-PRNP Monoclonal Antibody (DS2871AB) | WB, FC, IHC-P, ICC/IF | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
EPAF-0659LC | Recombinant Mouse Anti-Human PRNP Antibody (4F2) | WB | IgG |
EPAF-0984LC | Recombinant Mouse Anti-Human PRNP Antibody (mab 14D3) | ELISA | IgG |
EPAF-1254LC | Recombinant Mouse Anti-PRNP Antibody (V5B2) | WB | |
EPAF-0278CQ | Mouse Anti-PRNP Recombinant Antibody (clone 6BH) | Inhib | Mouse IgG1 |
EPAF-0279CQ | Mouse Anti-PRNP Recombinant Antibody (clone 3F10) | WB | Mouse IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0294-CN-F(E) | Human Anti-PRNP Recombinant Antibody (clone D18); Fab Fragment | WB, IF, IHC | Human Fab |
FAMAB-0295-CN-F(E) | Human Anti-PRNP Recombinant Antibody (clone 3:3); Fab Fragment | WB, IF, IHC | Human Fab |
FAMAB-0296-CN-F(E) | Human Anti-PRNP Recombinant Antibody (clone 6:4); Fab Fragment | WB, IF, IHC | Human Fab |
FAMAB-0297-CN-F(E) | Human Anti-PRNP Recombinant Antibody (clone 6:6); Fab Fragment | WB, IF, IHC | Human Fab |
HPAB-0282CQ-F(E) | Human Anti-PRNP Recombinant Antibody (clone boPrP-15); Fab Fragment | ELISA, WB | Human Fab |
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