Mouse Anti-PRNP Recombinant Antibody (clone 25G7)
CAT#: MOB-1227z
This product is a mouse antibody that recognizes human PRNP. It can be used in the following applications: WB, IP, IF, IHC, FCM, ELISA.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/if_selected.jpg
Subcellular Location
Figure 2 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42754/95550_B_7_5.jpg
Normal Tissue
Figure 4 Caudate
Neuronal cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/95554_B_9_7.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42754/95550_A_9_3.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (WM-115)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000171867-PRNP
❮
❯
Specifications
- Immunogen
- Human Prion Protein
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Human PRNP
- Species Reactivity
- Human
- Clone
- 25G7
- Applications
- WB, IP, IF, IHC, FC, ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western blot, Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Enzyme-linked Immunosorbent Assay.
Target
- Alternative Names
- PRNP; PrPc; PRP; Prion Protein; PrP33-35C; KURU; Prion-Related Protein; ASCR; Fatal Familial Insomnia; AltPrP; Prion Protein (P27-30); GSS; PRIP; PrP27-30; P27-30; CJD; Gerstmann-Strausler-Scheinker Syndrome; Creutzfeldt-Jakob Disease; CD230; CD230 Antige
- UniProt ID
- F7VJQ1
REVIEWS AND Q&AS
CITATIONS
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBC-107 | Mouse Anti-PRNP Recombinant Antibody (clone ICSM 18); scFv Fragment | ELISA | Mouse scFv |
| FAMAB-0294-CN-S(P) | Human Anti-PRNP Recombinant Antibody (clone D18); scFv Fragment | WB, IF, IHC | Human scFv |
| FAMAB-0295-CN-S(P) | Human Anti-PRNP Recombinant Antibody (clone 3:3); scFv Fragment | WB, IF, IHC | Human scFv |
| FAMAB-0296-CN-S(P) | Human Anti-PRNP Recombinant Antibody (clone 6:4); scFv Fragment | WB, IF, IHC | Human scFv |
| FAMAB-0297-CN-S(P) | Human Anti-PRNP Recombinant Antibody (clone 6:6); scFv Fragment | WB, IF, IHC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-943LC | Anti-Mouse Prnp Recombinant Antibody (D13) | ELISA | Human antibody |
| MRO-944LC | Anti-Mouse Prnp Recombinant Antibody (D18) | ELISA | Human antibody |
| MRO-945LC | Anti-Mouse Prnp Recombinant Antibody (R72) | ELISA | Human antibody |
| MRO-946LC | Anti-Mouse Prnp Recombinant Antibody (R1) | ELISA | Human antibody |
| MRO-947LC | Anti-Mouse Prnp Recombinant Antibody (R2) | ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-953LC | Anti-Human PRNP Recombinant Antibody (1A1) | FuncS, FC | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-942LC-F(E) | Anti-Sheep PRNP Recombinant Antibody Fab Fragment (6.1) | ELISA | Mouse antibody |
| MRO-948LC-F(E) | Anti-Mouse Prnp Recombinant Antibody Fab Fragment (6H10) | ELISA | Mouse antibody |
| MRO-949LC-F(E) | Anti-Mouse Prnp Recombinant Antibody Fab Fragment (31C6) | ELISA | Mouse antibody |
| PABX-161-S (P) | Recombinant Mouse Anti-PrP Antibody scFv Fragment (POM1) | WB, ELISA, FuncS | scFv |
| PABX-161-F (E) | Recombinant Mouse Anti-PrP Antibody Fab Fragment (POM1) | WB, ELISA, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0727MZ | Chicken Anti-PRNP Polyclonal IgY | ICC, IF | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2871 | Hi-Affi™ Recombinant Rabbit Anti-PRNP Monoclonal Antibody (DS2871AB) | WB, FC, IHC-P, ICC, IF | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0294-CN-F(E) | Human Anti-PRNP Recombinant Antibody (clone D18); Fab Fragment | WB, IF, IHC | Human Fab |
| FAMAB-0295-CN-F(E) | Human Anti-PRNP Recombinant Antibody (clone 3:3); Fab Fragment | WB, IF, IHC | Human Fab |
| FAMAB-0296-CN-F(E) | Human Anti-PRNP Recombinant Antibody (clone 6:4); Fab Fragment | WB, IF, IHC | Human Fab |
| FAMAB-0297-CN-F(E) | Human Anti-PRNP Recombinant Antibody (clone 6:6); Fab Fragment | WB, IF, IHC | Human Fab |
| HPAB-0909-FY-S(P) | Mouse Anti-PRNP Recombinant Antibody scFv Fragment (HPAB-0909-FY-S(P)) | WB, ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY84 | Human Anti-PRNP (clone mAb132) scFv-Fc Chimera | ELISA, WB | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1763 | Anti-PRNP Immunohistochemistry Kit | IHC | |
| VS-0525-XY5727 | Anti-Human PRNP Immunohistochemistry Kit | IHC | |
| VS-0525-XY5728 | Anti-Mouse PRNP Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC520 | Recombinant Anti-PRNP Vesicular Antibody, EV Displayed (VS-0425-YC520) | ELISA, FC, Inhib, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC150 | Recombinant Anti-PRNP (AA 56-90 x AA 83-89) Biparatopic Antibody, Tandem scFv (mAb 106 x mAb 110) | ELISA, IHC, WB | Tandem scFv |
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