Recombinant Anti-human PRNP Intrabody [(D-Arg)9]
CAT#: IAB-B034(A)
Recombinant anti-PRNP intrabody linked to (D-Arg)9 works within cells to bind PRNP, expressed in CHO cells. Anti-prion intrabodies targeted to the lumen of the endoplasmic reticulum provide a simple and effective means to inhibit the transport of PrPC to the cell surface.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/if_selected.jpg
Subcellular Location
Figure 2 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42754/95550_B_7_5.jpg
Normal Tissue
Figure 4 Caudate
Neuronal cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/95554_B_9_7.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42754/95550_A_9_3.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (WM-115)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000171867-PRNP
❮
❯
Specifications
- Immunogen
- human prion protein
- Host Species
- mouse
- Type
- scFv-(D-Arg)9
- Cell Penetrating Peptide
- (D-Arg)9
It has been proven that polyarginine peptides composed of nine arginine amino acids (Arg-9) are able to translocate across cells very efficiently. This raises the question if molecular dynamics simulations would show a similar pathway for this highly hydrophilic and charged peptide as predicted for the Tat peptide. The simplicity of Arg-9 over other CPPs also helps isolate the role of the Arg sidechain on the translocation without the effect of other sidechains. Therefore, in this work we study computationally the translocation of Arg-9 through a lipid bilayer and show that the translocation mechanism is the same as for the Tat peptide.
- Species Reactivity
- human
- Applications
- Can be useful in applications such as: Immunofluorescence; Flow Cytometry; Western blot; Functional studies
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1227z | Mouse Anti-PRNP Recombinant Antibody (clone 25G7) | WB, IP, IF, IHC, FC, ELISA | Mouse IgG1, κ |
| PABL-310 | Mouse Anti-PRNP Recombinant Antibody (clone POM2) | WB, IHC, FuncS | Mouse IgG |
| MOB-1629CT | Recombinant Mouse anti-Human PRNP Monoclonal antibody (6C0) | ELISA, WB | |
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| MRO-1266-CN | Recombinant Rabbit Anti-PRNP Monoclonal Antibody (CBACN-457) | WB, IF, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B034(G) | Recombinant Anti-human PRNP Intrabody [+36 GFP] | WB, CO-IP, FuncS | scFv-(+36GFP) |
| IAB-B034(T) | Recombinant Anti-human PRNP Intrabody [Tat] | IF, ELISA, FuncS | scFv-Tat |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PNBL-026 | Recombinant Anti-Human PRNP VHH Single Domain Antibody (PNBL-026) | WB, ELISA, FuncS | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-953LC | Anti-Human PRNP Recombinant Antibody (1A1) | FuncS, FC | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-954LC | Anti-Human PRNP Recombinant Antibody (V5B2) | ELISA | Mouse antibody |
| MRO-955LC | Mouse Anti-Prnp Recombinant Antibody (MRO-955LC) | ELISA, WB | Mouse IgG |
| MRO-956LC | Mouse Anti-Prnp Recombinant Antibody (MRO-956LC) | ELISA, WB | Mouse IgG |
| MRO-957LC | Mouse Anti-Prnp Recombinant Antibody (MRO-957LC) | ELISA, WB | Mouse IgG |
| MRO-942LC-S(P) | Anti-Sheep PRNP Recombinant Antibody scFv Fragment (6.1) | ELISA | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-943LC-S(P) | Anti-Mouse Prnp Recombinant Antibody scFv Fragment (D13) | ELISA | Human antibody |
| MRO-944LC-S(P) | Anti-Mouse Prnp Recombinant Antibody scFv Fragment (D18) | ELISA | Human antibody |
| MRO-945LC-S(P) | Anti-Mouse Prnp Recombinant Antibody scFv Fragment (R72) | ELISA | Human antibody |
| MRO-946LC-S(P) | Anti-Mouse Prnp Recombinant Antibody scFv Fragment (R1) | ELISA | Human antibody |
| MRO-947LC-S(P) | Anti-Mouse Prnp Recombinant Antibody scFv Fragment (R2) | ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0727MZ | Chicken Anti-PRNP Polyclonal IgY | ICC, IF | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2871 | Hi-Affi™ Recombinant Rabbit Anti-PRNP Monoclonal Antibody (DS2871AB) | WB, FC, IHC-P, ICC, IF | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0272CQ-F(E) | Human Anti-PRNP Recombinant Antibody (clone ch31C6); Fab Fragment | ELISA, WB, IHC | Chimeric (mouse/human) Fab |
| FAMAB-0273CQ-F(E) | Mouse Anti-PRNP Recombinant Antibody (clone mAb 106); Fab Fragment | ELISA, WB, IHC | Mouse Fab |
| FAMAB-0274CQ-F(E) | Mouse Anti-PRNP Recombinant Antibody (clone mAb 110); Fab Fragment | ELISA, WB, IHC | Mouse Fab |
| FAMAB-0275CQ-F(E) | Mouse Anti-PRNP Recombinant Antibody (clone mAb 132); Fab Fragment | ELISA, WB, IHC | Mouse Fab |
| HPAB-0282CQ-F(E) | Human Anti-PRNP Recombinant Antibody (clone boPrP-15); Fab Fragment | ELISA, WB | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0050-CN-S(P) | Chicken Anti-PRNP Recombinant Antibody (clone phAb4-31); scFv Fragment | ELISA | Chicken scFv |
| FAMAB-0051-CN-S(P) | Human Anti-PRNP Recombinant Antibody (clone hu4.00); scFv Fragment | ELISA | Humanized scFv |
| FAMAB-0052-CN-S(P) | Chicken Anti-PRNP Recombinant Antibody (clone ch4.31); scFv Fragment | ELISA | Chicken scFv |
| FAMAB-0053-CN-S(P) | Human Anti-PRNP Recombinant Antibody (clone hu4.15); scFv Fragment | ELISA | Human scFv |
| FAMAB-0054-CN-S(P) | Human Anti-PRNP Recombinant Antibody (clone hu4.44); scFv Fragment | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY84 | Human Anti-PRNP (clone mAb132) scFv-Fc Chimera | ELISA, WB | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1763 | Anti-PRNP Immunohistochemistry Kit | IHC | |
| VS-0525-XY5727 | Anti-Human PRNP Immunohistochemistry Kit | IHC | |
| VS-0525-XY5728 | Anti-Mouse PRNP Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC520 | Recombinant Anti-PRNP Vesicular Antibody, EV Displayed (VS-0425-YC520) | ELISA, FC, Inhib, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC150 | Recombinant Anti-PRNP (AA 56-90 x AA 83-89) Biparatopic Antibody, Tandem scFv (mAb 106 x mAb 110) | ELISA, IHC, WB | Tandem scFv |
| VS-0525-YC152 | Recombinant Anti-PRNP (AA 119-127 x AA 143-149) Biparatopic Antibody, Tandem scFv (mAb 132 x Clone 31C6) | ELISA, IHC, WB | Tandem scFv |
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