Human Anti-PRNP (clone mAb132) scFv-Fc Chimera
CAT#: VS-0125-FY84
This is an engineered antibody-like molecule that combines the specificity of a single-chain variable fragment (scFv) with the stability and effector functions of the human IgG1 Fc region. The scFv portion is derived from a chicken monoclonal antibody that specifically recognizes human prion proteins, making it particularly valuable in research and diagnostic applications related to prion diseases, such as variant Creutzfeldt-Jakob disease.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/if_selected.jpg
Subcellular Location
Figure 2 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42754/95550_B_7_5.jpg
Normal Tissue
Figure 4 Caudate
Neuronal cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/95554_B_9_7.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42754/95550_A_9_3.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (WM-115)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000171867-PRNP
❮
❯
Specifications
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Species Reactivity
- Human
- Clone
- mAb132
- Applications
- ELISA, Western Blot
- Related Disease
- Related to prion diseases, such as variant creutzfeldt-Jakob disease
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Stabilizer
- No Stabilizers
- Sterility
- 0.2 μM filtered
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- CJD, GSS, PrP, ASCR, KURU, PRIP, PrPc, CD230, AltPrP, p27-30, PrP27-30, PrP33-35C
- Gene ID
- 5621
- UniProt ID
- P04156
- Sequence Similarities
- Belongs to the prion family.
- Cellular Localization
- Cell membrane; Lipid-anchor, GPI-anchor
Golgi apparatus
- Post Translation Modifications
- The glycosylation pattern (the amount of mono-, di- and non-glycosylated forms or glycoforms) seems to differ in normal and CJD prion.
Glycosylation at Thr95, Asn181, Thr193, and Asn197
Modification sites at PhosphoSitePlus
Glycosylation from GlyConnect PRIO_HUMAN (2056)
Glycosylation from GlyGen 4 sites, 12 N-linked glycans (2 sites), 2 O-linked glycans (2 sites)
- Protein Refseq
- NP_000302.1; NP_001073590.1; NP_001073591.1; NP_001073592.1; NP_001258490.1
- Function
- Its primary physiological function is unclear.
May play a role in neuronal development and synaptic plasticity.
May be required for neuronal myelin sheath maintenance.
May promote myelin homeostasis through acting as an agonist for ADGRG6 receptor.
May play a role in iron uptake and iron homeostasis.
Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro) (By similarity).
Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts.
Also provides Cu(2+) or Zn(2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-310 | Mouse Anti-PRNP Recombinant Antibody (clone POM2); scFv Fragment | WB, IHC, FuncS | Mouse scFv |
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| HPAB-0909-FY-F(E) | Mouse Anti-PRNP Recombinant Antibody Fab Fragment (HPAB-0909-FY-F(E)) | WB, ELISA | Mouse Fab |
| PABX-161-S(P) | Mouse Anti-PRNP Recombinant Antibody (clone POM1); scFv Fragment | ELISA, IP, IHC, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PNBL-026 | Recombinant Anti-Human PRNP VHH Single Domain Antibody (PNBL-026) | WB, ELISA, FuncS | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABW-089 | Mouse Anti-PRNP Recombinant Antibody (clone 3F4) | ELISA | Mouse IgG2a |
| FAMAB-0294-CN | Human Anti-PRNP Recombinant Antibody (clone D18) | WB, IF, IHC | Human IgG |
| FAMAB-0295-CN | Human Anti-PRNP Recombinant Antibody (clone 3:3) | WB, IF, IHC | Human IgG |
| FAMAB-0296-CN | Human Anti-PRNP Recombinant Antibody (clone 6:4) | WB, IF, IHC | Human IgG |
| HPAB-0909-FY | Mouse Anti-PRNP Recombinant Antibody (HPAB-0909-FY) | WB, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-953LC | Anti-Human PRNP Recombinant Antibody (1A1) | FuncS, FC | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-942LC-F(E) | Anti-Sheep PRNP Recombinant Antibody Fab Fragment (6.1) | ELISA | Mouse antibody |
| MRO-948LC-F(E) | Anti-Mouse Prnp Recombinant Antibody Fab Fragment (6H10) | ELISA | Mouse antibody |
| MRO-949LC-F(E) | Anti-Mouse Prnp Recombinant Antibody Fab Fragment (31C6) | ELISA | Mouse antibody |
| MRO-950LC-F(E) | Anti-Mouse Prnp Recombinant Antibody Fab Fragment (72-5) | ELISA | Mouse antibody |
| PABX-161-F (E) | Recombinant Mouse Anti-PrP Antibody Fab Fragment (POM1) | WB, ELISA, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-943LC-F(E) | Anti-Mouse Prnp Recombinant Antibody Fab Fragment (D13) | ELISA | Human antibody |
| MRO-944LC-F(E) | Anti-Mouse Prnp Recombinant Antibody Fab Fragment (D18) | ELISA | Human antibody |
| MRO-945LC-F(E) | Anti-Mouse Prnp Recombinant Antibody Fab Fragment (R72) | ELISA | Human antibody |
| MRO-946LC-F(E) | Anti-Mouse Prnp Recombinant Antibody Fab Fragment (R1) | ELISA | Human antibody |
| MRO-947LC-F(E) | Anti-Mouse Prnp Recombinant Antibody Fab Fragment (R2) | ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0727MZ | Chicken Anti-PRNP Polyclonal IgY | ICC, IF | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2871 | Hi-Affi™ Recombinant Rabbit Anti-PRNP Monoclonal Antibody (DS2871AB) | WB, FC, IHC-P, ICC, IF | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0294-CN-F(E) | Human Anti-PRNP Recombinant Antibody (clone D18); Fab Fragment | WB, IF, IHC | Human Fab |
| FAMAB-0295-CN-F(E) | Human Anti-PRNP Recombinant Antibody (clone 3:3); Fab Fragment | WB, IF, IHC | Human Fab |
| FAMAB-0296-CN-F(E) | Human Anti-PRNP Recombinant Antibody (clone 6:4); Fab Fragment | WB, IF, IHC | Human Fab |
| FAMAB-0297-CN-F(E) | Human Anti-PRNP Recombinant Antibody (clone 6:6); Fab Fragment | WB, IF, IHC | Human Fab |
| HPAB-1462LY-F(E) | Mouse Anti-PRNP Recombinant Antibody (clone CBL030); Fab Fragment | ELISA, WB, Inhib | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1763 | Anti-PRNP Immunohistochemistry Kit | IHC | |
| VS-0525-XY5727 | Anti-Human PRNP Immunohistochemistry Kit | IHC | |
| VS-0525-XY5728 | Anti-Mouse PRNP Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC520 | Recombinant Anti-PRNP Vesicular Antibody, EV Displayed (VS-0425-YC520) | ELISA, FC, Inhib, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC150 | Recombinant Anti-PRNP (AA 56-90 x AA 83-89) Biparatopic Antibody, Tandem scFv (mAb 106 x mAb 110) | ELISA, IHC, WB | Tandem scFv |
| VS-0525-YC152 | Recombinant Anti-PRNP (AA 119-127 x AA 143-149) Biparatopic Antibody, Tandem scFv (mAb 132 x Clone 31C6) | ELISA, IHC, WB | Tandem scFv |
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