Anti-PRNP Intrabody [Tat] (IAB-B034(T))
Recombinant anti-PRNP intrabody in fusion with HIV Tat works within cells to bind PRNP, expressed in CHO cells. Anti-prion intrabodies targeted to the lumen of the endoplasmic reticulum provide a simple and effective means to inhibit the transport of PrPC to the cell surface.
Specifications
- Immunogen
- human prion protein
- Host
- mouse
- Type
- scFv-Tat
- Cell Penetrating Peptide
- TAT
TAT was able to deliver different proteins, such as horseradish peroxidase and RNase A across cell membrane into the cytoplasm in different cell lines in vitro. The size range of proteins with effective delivery is from 30kDa to 120-150kDa. In one study, TAT-fused proteins are rapidly internalized by lipid raft−dependent macropinocytosis using a transducible TAT−Cre recombinase reporter assay on live cells. In another study, a TAT-fused protein was delivered into mitochondria of breast cancer cells and decreased the survival of breast cancer cells, which showed capability of TAT-fusion proteins to modulate mitochondrial function and cell survival. However, very few in vivo studies have succeeded. In one study, in vivo delivery of TAT- or penetratin-crosslinked Fab fragments yielded varied organ distributions and an overall increase in organ retention, which showed tissue localization.
- Species Reactivity
- human
- Applications
- Antibody assay: Immunofluorescence; Enzyme-linked Immunosorbent Assay; Functional studies
Target
- Alternative Names
- PRNP; prion protein; CJD; GSS; PrP; ASCR; KURU; PRIP
- Entrez Gene ID
- 5621
- UniProt ID
- P04156
For lab research use only, not for diagnostic, therapeutic or any in vivo human use.
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