Recombinant Anti-human PRNP Intrabody [Tat]
CAT#: IAB-B034(T)
Recombinant anti-PRNP intrabody in fusion with HIV Tat works within cells to bind PRNP, expressed in CHO cells. Anti-prion intrabodies targeted to the lumen of the endoplasmic reticulum provide a simple and effective means to inhibit the transport of PrPC to the cell surface.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/if_selected.jpg
Subcellular Location
Figure 2 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42754/95550_B_7_5.jpg
Normal Tissue
Figure 4 Caudate
Neuronal cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/95554_B_9_7.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42754/95550_A_9_3.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (WM-115)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000171867-PRNP
❮
❯
Specifications
- Immunogen
- human prion protein
- Host Species
- mouse
- Type
- scFv-Tat
- Cell Penetrating Peptide
- TAT
TAT was able to deliver different proteins, such as horseradish peroxidase and RNase A across cell membrane into the cytoplasm in different cell lines in vitro. The size range of proteins with effective delivery is from 30kDa to 120-150kDa. In one study, TAT-fused proteins are rapidly internalized by lipid raft−dependent macropinocytosis using a transducible TAT−Cre recombinase reporter assay on live cells. In another study, a TAT-fused protein was delivered into mitochondria of breast cancer cells and decreased the survival of breast cancer cells, which showed capability of TAT-fusion proteins to modulate mitochondrial function and cell survival. However, very few in vivo studies have succeeded. In one study, in vivo delivery of TAT- or penetratin-crosslinked Fab fragments yielded varied organ distributions and an overall increase in organ retention, which showed tissue localization.
- Species Reactivity
- human
- Applications
- Antibody assay: Immunofluorescence; Enzyme-linked Immunosorbent Assay; Functional studies
Product Property
- Purity
- >97%, by SDS-PAGE under reducing conditions
- Storage
- Short Term Storage: 4°C
Long Term Storage: -20°C
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for IAB-B034(T). Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "PRNP"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B034(A) | Recombinant Anti-human PRNP Intrabody [(D-Arg)9] | IF, FC, WB, FuncS | scFv-(D-Arg)9 |
| IAB-B034(G) | Recombinant Anti-human PRNP Intrabody [+36 GFP] | WB, CO-IP, FuncS | scFv-(+36GFP) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-310 | Mouse Anti-PRNP Recombinant Antibody (clone POM2); Fab Fragment | WB, IHC, FuncS | Mouse Fab |
| FAMAB-0294-CN-F(E) | Human Anti-PRNP Recombinant Antibody (clone D18); Fab Fragment | WB, IF, IHC | Human Fab |
| FAMAB-0295-CN-F(E) | Human Anti-PRNP Recombinant Antibody (clone 3:3); Fab Fragment | WB, IF, IHC | Human Fab |
| FAMAB-0296-CN-F(E) | Human Anti-PRNP Recombinant Antibody (clone 6:4); Fab Fragment | WB, IF, IHC | Human Fab |
| FAMAB-0297-CN-F(E) | Human Anti-PRNP Recombinant Antibody (clone 6:6); Fab Fragment | WB, IF, IHC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PNBL-026 | Recombinant Anti-Human PRNP VHH Single Domain Antibody (PNBL-026) | WB, ELISA, FuncS | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-943LC | Anti-Mouse Prnp Recombinant Antibody (D13) | ELISA | Human antibody |
| MRO-944LC | Anti-Mouse Prnp Recombinant Antibody (D18) | ELISA | Human antibody |
| MRO-945LC | Anti-Mouse Prnp Recombinant Antibody (R72) | ELISA | Human antibody |
| MRO-946LC | Anti-Mouse Prnp Recombinant Antibody (R1) | ELISA | Human antibody |
| MRO-947LC | Anti-Mouse Prnp Recombinant Antibody (R2) | ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-953LC | Anti-Human PRNP Recombinant Antibody (1A1) | FuncS, FC | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-942LC-S(P) | Anti-Sheep PRNP Recombinant Antibody scFv Fragment (6.1) | ELISA | Mouse antibody |
| MRO-948LC-S(P) | Anti-Mouse Prnp Recombinant Antibody scFv Fragment (6H10) | ELISA | Mouse antibody |
| MRO-949LC-S(P) | Anti-Mouse Prnp Recombinant Antibody scFv Fragment (31C6) | ELISA | Mouse antibody |
| MRO-950LC-S(P) | Anti-Mouse Prnp Recombinant Antibody scFv Fragment (72-5) | ELISA | Mouse antibody |
| PABX-161-F (E) | Recombinant Mouse Anti-PrP Antibody Fab Fragment (POM1) | WB, ELISA, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0727MZ | Chicken Anti-PRNP Polyclonal IgY | ICC, IF | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-161 | Mouse Anti-PRNP Recombinant Antibody (clone POM1) | ELISA, IP, IHC, FuncS | Mouse IgG1 |
| HPAB-0282CQ | Human Anti-PRNP Recombinant Antibody (clone boPrP-15) | ELISA, WB | Human IgG |
| HPAB-0283CQ | Human Anti-PRNP Recombinant Antibody (clone huPrP-18) | ELISA, WB | Human IgG |
| MRO-1266-CN | Recombinant Rabbit Anti-PRNP Monoclonal Antibody (CBACN-457) | WB, IF, IHC, FC | Rabbit IgG |
| HPAB-0909-FY | Mouse Anti-PRNP Recombinant Antibody (HPAB-0909-FY) | WB, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2871 | Hi-Affi™ Recombinant Rabbit Anti-PRNP Monoclonal Antibody (DS2871AB) | WB, FC, IHC-P, ICC, IF | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-0594CQ | Recombinant Mouse Anti-Human PRNP Antibody (ICSM68) | ELISA | IgG |
| EPAF-0595CQ | Recombinant Mouse Anti-Human PRNP Antibody (ICSM39) | ELISA | IgG |
| EPAF-0596CQ | Recombinant Mouse Anti-Human PRNP Antibody (ION6) | ELISA | IgG |
| EPAF-0597CQ | Recombinant Mouse Anti-Human PRNP Antibody (ICSM56) | ELISA | IgG1 |
| EPAF-0598CQ | Recombinant Mouse Anti-Human PRNP Antibody (ICSM72) | ELISA | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0282CQ-S(P) | Human Anti-PRNP Recombinant Antibody (clone boPrP-15); scFv Fragment | ELISA, WB | Human scFv |
| HPAB-0283CQ-S(P) | Human Anti-PRNP Recombinant Antibody (clone huPrP-18); scFv Fragment | ELISA, WB | Human scFv |
| HPAB-0284CQ-S(P) | Human Anti-PRNP Recombinant Antibody (clone huPrP-39); scFv Fragment | ELISA, WB | Human scFv |
| HPAB-0285CQ-S(P) | Human Anti-PRNP Recombinant Antibody (clone boPrP-20); scFv Fragment | ELISA, WB | Human scFv |
| HPAB-0909-FY-F(E) | Mouse Anti-PRNP Recombinant Antibody Fab Fragment (HPAB-0909-FY-F(E)) | WB, ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY84 | Human Anti-PRNP (clone mAb132) scFv-Fc Chimera | ELISA, WB | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1763 | Anti-PRNP Immunohistochemistry Kit | IHC | |
| VS-0525-XY5727 | Anti-Human PRNP Immunohistochemistry Kit | IHC | |
| VS-0525-XY5728 | Anti-Mouse PRNP Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC520 | Recombinant Anti-PRNP Vesicular Antibody, EV Displayed (VS-0425-YC520) | ELISA, FC, Inhib, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC150 | Recombinant Anti-PRNP (AA 56-90 x AA 83-89) Biparatopic Antibody, Tandem scFv (mAb 106 x mAb 110) | ELISA, IHC, WB | Tandem scFv |
| VS-0525-YC152 | Recombinant Anti-PRNP (AA 119-127 x AA 143-149) Biparatopic Antibody, Tandem scFv (mAb 132 x Clone 31C6) | ELISA, IHC, WB | Tandem scFv |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: ELISA, IHC
Application: ELISA, WB, BLI, SPR
Application: WB, IF, FuncS
-2.png)
Application: ELISA, WB
Application: ELISA, FuncS, Neut, IF, WB, EM, Inhib, IHC
Application: ELISA, FC, Inhib, IHC-Fr, WB, IP
Application: ELISA, IHC, IF, IP
Application: ELISA, Inhib, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
