Recombinant Anti-human PRNP Intrabody [Tat] (CAT#: IAB-B034(T))
Recombinant anti-PRNP intrabody in fusion with HIV Tat works within cells to bind PRNP, expressed in CHO cells. Anti-prion intrabodies targeted to the lumen of the endoplasmic reticulum provide a simple and effective means to inhibit the transport of PrPC to the cell surface.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/if_selected.jpg
Figure 2 IHC staining of human cerebellum
(Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/ihc_selected.jpg
(Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/ihc_selected.jpg
Figure 3 Cerebral cortex
(Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42754/95550_B_7_5.jpg
(Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42754/95550_B_7_5.jpg
Figure 4 Caudate
(Neuronal cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/95554_B_9_7.jpg
(Neuronal cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43398/95554_B_9_7.jpg
Figure 5 Colon
(Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42754/95550_A_9_3.jpg
(Endothelial cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/42754/95550_A_9_3.jpg
Figure 6 RNA cell line category: Cell line enhanced (WM-115)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000171867-PRNP
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000171867-PRNP
Figure 6 RNA cell line category: Cell line enhanced (WM-115)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000171867-PRNP
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000171867-PRNP
Specifications
- Immunogen
- human prion protein
- Host Species
- mouse
- Type
- scFv-Tat
- Cell Penetrating Peptide
- TAT
TAT was able to deliver different proteins, such as horseradish peroxidase and RNase A across cell membrane into the cytoplasm in different cell lines in vitro. The size range of proteins with effective delivery is from 30kDa to 120-150kDa. In one study, TAT-fused proteins are rapidly internalized by lipid raft−dependent macropinocytosis using a transducible TAT−Cre recombinase reporter assay on live cells. In another study, a TAT-fused protein was delivered into mitochondria of breast cancer cells and decreased the survival of breast cancer cells, which showed capability of TAT-fusion proteins to modulate mitochondrial function and cell survival. However, very few in vivo studies have succeeded. In one study, in vivo delivery of TAT- or penetratin-crosslinked Fab fragments yielded varied organ distributions and an overall increase in organ retention, which showed tissue localization.
- Species Reactivity
- human
- Applications
- Antibody assay: Immunofluorescence; Enzyme-linked Immunosorbent Assay; Functional studies
Product Property
- Purity
- >97%, by SDS-PAGE under reducing conditions
- Storage
- Short Term Storage: 4°C
Long Term Storage: -20°C
Target
- Alternative Names
- PRNP; prion protein; CJD; GSS; PrP; ASCR; KURU; PRIP; PrPc; CD230; AltPrP; p27-30; PrP27-30; PrP33-35C; major prion protein; CD230 antigen; prion-related protein
- Gene ID
- 5621
- UniProt ID
- P04156
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "PRNP"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1227z | Mouse Anti-PRNP Recombinant Antibody (clone 25G7) | WB, IP, IF, IHC, FC, ELISA | Mouse IgG1, κ |
| PABW-089 | Mouse Anti-PRNP Recombinant Antibody (clone 3F4) | ELISA | Mouse IgG2a |
| PABC-107 | Mouse Anti-PRNP Recombinant Antibody (clone ICSM 18) | ELISA, Inhib, IF | Mouse IgG |
| PABW-107 | Mouse Anti-PRNP Recombinant Antibody (clone VRQ14) | ELISA | Mouse IgG2a |
| PABX-161 | Mouse Anti-PRNP Recombinant Antibody (clone POM1) | ELISA, IP, IHC, FuncS | Mouse IgG1 |
Intrabody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B034(A) | Recombinant Anti-human PRNP Intrabody [(D-Arg)9] | IF, FC, WB, FuncS | scFv-(D-Arg)9 |
| IAB-B034(G) | Recombinant Anti-human PRNP Intrabody [+36 GFP] | WB, CO-IP, FuncS | scFv-(+36GFP) |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PNBL-026 | Recombinant Anti-Human PRNP VHH Single Domain Antibody (Nb484) | WB, ELISA, FuncS | Llama VHH |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBW-089 | Mouse Anti-PRNP Recombinant Antibody (clone 3F4); Fab Fragment | ELISA | Mouse Fab |
| FAMAB-0050-CN-F(E) | Chicken Anti-PRNP Recombinant Antibody (clone phAb4-31); Fab Fragment | ELISA | Chicken Fab |
| FAMAB-0051-CN-F(E) | Human Anti-PRNP Recombinant Antibody (clone hu4.00); Fab Fragment | ELISA | Humanized Fab |
| FAMAB-0052-CN-F(E) | Human Anti-PRNP Recombinant Antibody (clone ch4.31); Fab Fragment | ELISA | Chimeric (chicken/human) Fab |
| FAMAB-0053-CN-F(E) | Human Anti-PRNP Recombinant Antibody (clone hu4.15); Fab Fragment | ELISA | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBW-089 | Mouse Anti-PRNP Recombinant Antibody (clone 3F4); scFv Fragment | ELISA | Mouse scFv |
| PSBC-107 | Mouse Anti-PRNP Recombinant Antibody (clone ICSM 18); scFv Fragment | ELISA | Mouse scFv |
| PSBW-107 | Mouse Anti-PRNP Recombinant Antibody (clone VRQ14); scFv Fragment | ELISA | Mouse scFv |
| FAMAB-0054-CN-S(P) | Human Anti-PRNP Recombinant Antibody (clone hu4.44); scFv Fragment | ELISA | Human scFv |
| HPAB-0909-FY-F(E) | Mouse Anti-PRNP Recombinant Antibody (clone APR3); scFv Fragment | WB, ELISA | Mouse scFv |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-943LC | Anti-Mouse Prnp Recombinant Antibody (D13) | ELISA | Human antibody |
| MRO-944LC | Anti-Mouse Prnp Recombinant Antibody (D18) | ELISA | Human antibody |
| MRO-945LC | Anti-Mouse Prnp Recombinant Antibody (R72) | ELISA | Human antibody |
| MRO-946LC | Anti-Mouse Prnp Recombinant Antibody (R1) | ELISA | Human antibody |
| MRO-947LC | Anti-Mouse Prnp Recombinant Antibody (R2) | ELISA | Human antibody |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-942LC-F(E) | Anti-Sheep PRNP Recombinant Antibody Fab Fragment (6.1) | ELISA | Mouse antibody |
| MRO-948LC-F(E) | Anti-Mouse Prnp Recombinant Antibody Fab Fragment (6H10) | ELISA | Mouse antibody |
| MRO-949LC-F(E) | Anti-Mouse Prnp Recombinant Antibody Fab Fragment (31C6) | ELISA | Mouse antibody |
| MRO-950LC-F(E) | Anti-Mouse Prnp Recombinant Antibody Fab Fragment (72-5) | ELISA | Mouse antibody |
| MRO-951LC-F(E) | Anti-Mouse Prnp Recombinant Antibody Fab Fragment (44B1) | ELISA | Mouse antibody |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0727MZ | Chicken Anti-PRNP Polyclonal IgY | ICC/IF | Chicken antibody |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2871 | Hi-Affi™ Recombinant Rabbit Anti-PRNP Monoclonal Antibody (DS2871AB) | WB, FC, IHC-P, ICC/IF | IgG |
Epitope-Specific Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-0613CQ | Recombinant Mouse Anti-Human PRNP Antibody (6F4) | FC | IgG1, κ |
| EPAF-0614CQ | Recombinant Mouse Anti-Human PRNP Antibody (8A4) | FC | IgG2b, κ |
| EPAF-0615CQ | Recombinant Mouse Anti-Human PRNP Antibody (8E7) | FC | IgG1, κ |
| EPAF-0616CQ | Recombinant Mouse Anti-Human PRNP Antibody (2D10) | IHC, WB, FC | IgG2b, κ |
| EPAF-0617CQ | Recombinant Mouse Anti-Human PRNP Antibody (2E7) | IHC, WB, FC | IgG2a, κ |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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