Z-domain of protein A scaffold protein anti-Human CD28 (CAT#: SZA-L216)

This CD28 specific binding protein (Z-domain of protein A) was investigated by an α-helix shuffling strategy. The primary scaffold protein was from a naive combinatorial library of the three-helix bundle Z domain derived from staphylococcal protein A. A hierarchical library was constructed through selective re-randomization of six amino acid positions in one of the two α-helices of the domain, making up the Taq DNA polymerase binding surface. After selections using monovalent phage display technology, second generation variants were identified having affinities (KD=8.5 μM) for CD28 as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.