Z-domain of protein A scaffold protein anti-Human CD28

Name: Z-domain of protein A scaffold protein anti-Human CD28
SKU: SZA-L216
Rating: 5 (1 reviews)

CAT#: SZA-L216

This CD28 specific binding protein (Z-domain of protein A) was investigated by an α-helix shuffling strategy. The primary scaffold protein was from a naive combinatorial library of the three-helix bundle Z domain derived from staphylococcal protein A. A hierarchical library was constructed through selective re-randomization of six amino acid positions in one of the two α-helices of the domain, making up the Taq DNA polymerase binding surface. After selections using monovalent phage display technology, second generation variants were identified having affinities (KD=8.5 μM) for CD28 as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71425/1522_F4_1_selected.jpg

RNA Expression Figure 2 RNA cell line category: Cell line enriched (HDLM-2)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000049249-TNFRSF9

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Specifications

Scaffold Name
Z-domain of protein A

Origin
Staphylococcal protein A

Core Structure
3-α helixes

Variable Regions
13 Residues on first and second helix surface

Target
CD28

Species Reactivity
Human

Expression Host
BL21 (DE3) cells

Affinity Constant
8.5 μM

Applications
ELISA; IHC; Microscopy; FC; WB; FuncS

Target

Alternative Names
Staphylococcal protein A; Z-domain of protein A; TNFRSF9; tumor necrosis factor receptor superfamily, member 9; ILA; 4-1BB; CD137; CDw137; tumor necrosis factor receptor superfamily member 9; CD137 antigen; T cell antigen ILA; T-cell antigen ILA; 4-1BB ligand receptor; homolog of mouse 4-1BB; receptor protein 4-1BB; T-cell antigen 4-1BB homolog; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63

Gene ID
3604

UniProt ID
Q07011

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Datasheet

Datasheet - SZA-L216.pdf

MSDS

MSDS - SZA-L216.pdf

COA

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