Anti-Human TNFRSF9 Recombinant Antibody (TAB-457CQ) (CAT#: TAB-457CQ)

Recombinant monoclonal antibody to TNFRSF9. This antibody is a human monoclonal antibody that specifically binds to the CD137 receptor (also called 4-1BB).

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WB

Figure 1 Anti-Human TNFRSF9 Recombinant Antibody (TAB-457CQ) in WB

Figure 1 Anti-Human TNFRSF9 Recombinant Antibody (TAB-457CQ) in WB

Western blot analysis of TAB-457CQ was performed with Recombinant Human TNFRSF9 Protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-457CQ and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.

ELISA

Figure 2 Anti-Human TNFRSF9 Recombinant Antibody (TAB-457CQ) in ELISA

Figure 2 Anti-Human TNFRSF9 Recombinant Antibody (TAB-457CQ) in ELISA

ELISA analysis of TAB-457CQ was performed by coating with Recombinant Human TNFRSF9 Protein. Then blocked with BSA, and incubated with Anti-Human TNFRSF9 Antibody (TAB-457CQ). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

SDS-PAGE

Figure 3 Anti-Human TNFRSF9 Recombinant Antibody (TAB-457CQ)-Low endotoxin in SDS-PAGE

Figure 3 Anti-Human TNFRSF9 Recombinant Antibody (TAB-457CQ)-Low endotoxin in SDS-PAGE

SDS-PAGE analysis of TAB-457CQ-LowE in β-mercaptoethanol-reduced (Lane 2) and non-reduced (Lane 1) conditions.

WB

Figure 4 Anti-Human TNFRSF9 Recombinant Antibody (TAB-457CQ)-Low endotoxin in WB

Figure 4 Anti-Human TNFRSF9 Recombinant Antibody (TAB-457CQ)-Low endotoxin in WB

Western blot analysis of TAB-457CQ-LowE was performed by loading human TNFRSF9 protein, His tag.

ELISA

Figure 5 Anti-Human TNFRSF9 Recombinant Antibody (TAB-457CQ)-Low endotoxin in ELISA

Figure 5 Anti-Human TNFRSF9 Recombinant Antibody (TAB-457CQ)-Low endotoxin in ELISA

ELISA analysis of TAB-457CQ-LowE was performed by coating with human TNFRSF9 protein (His tag). Then blocked with BSA and incubated with TAB-457CQ-LowE. The HRP conjugated Anti-human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.


Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • IgG2, λ
  • Specificity
  • Human receptor 4-1BB
  • Species Reactivity
  • Human
  • Applications
  • ELISA, IHC, FC, IP, IF, FuncS
  • Conjugate
  • Unconjugated
  • Related Disease
  • B cell lymphoma, Non-Hodgkin's lymphoma, Solid tumours

Product Property

  • Purity
  • > 95% as determined by SDS-PAGE
  • Storage
  • Store at -20°C for long-term storage. Avoid freeze/thaw cycles.

Applications

  • Application Notes
  • The TNFRSF9 antibody has been reported in applications of SPR, Activ, IF.

Target

  • Alternative Names
  • TNFRSF9; tumor necrosis factor receptor superfamily, member 9; ILA; 4-1BB; CD137; CDw137; tumor necrosis factor receptor superfamily member 9; CD137 antigen; T cell antigen ILA; T-cell antigen ILA; 4-1BB ligand receptor; homolog of mouse 4-1BB; receptor protein 4-1BB; T-cell antigen 4-1BB homolog; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63

Related Resources

  • Biosimilar Overview
Please refer to Utomilumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Utomilumab.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-457CQ, RRID: AB_3111955)

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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