Anti-Human TNFRSF9 Recombinant Antibody (TAB-457CQ) (CAT#: TAB-457CQ)
Recombinant monoclonal antibody to TNFRSF9. This antibody is a human monoclonal antibody that specifically binds to the CD137 receptor (also called 4-1BB).
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WB
Figure 1 Anti-Human TNFRSF9 Recombinant Antibody (TAB-457CQ) in WB
Western blot analysis of TAB-457CQ was performed with Recombinant Human TNFRSF9 Protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-457CQ and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
ELISA
Figure 2 Anti-Human TNFRSF9 Recombinant Antibody (TAB-457CQ) in ELISA
ELISA analysis of TAB-457CQ was performed by coating with Recombinant Human TNFRSF9 Protein. Then blocked with BSA, and incubated with Anti-Human TNFRSF9 Antibody (TAB-457CQ). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
SDS-PAGE
Figure 3 Anti-Human TNFRSF9 Recombinant Antibody (TAB-457CQ)-Low endotoxin in SDS-PAGE
SDS-PAGE analysis of TAB-457CQ-LowE in β-mercaptoethanol-reduced (Lane 2) and non-reduced (Lane 1) conditions.
WB
Figure 4 Anti-Human TNFRSF9 Recombinant Antibody (TAB-457CQ)-Low endotoxin in WB
Western blot analysis of TAB-457CQ-LowE was performed by loading human TNFRSF9 protein, His tag.
ELISA
Figure 5 Anti-Human TNFRSF9 Recombinant Antibody (TAB-457CQ)-Low endotoxin in ELISA
ELISA analysis of TAB-457CQ-LowE was performed by coating with human TNFRSF9 protein (His tag). Then blocked with BSA and incubated with TAB-457CQ-LowE. The HRP conjugated Anti-human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Derivation
- Human
- Type
- IgG2, λ
- Specificity
- Human receptor 4-1BB
- Species Reactivity
- Human
- Applications
- ELISA, IHC, FC, IP, IF, FuncS
- Conjugate
- Unconjugated
- Related Disease
- B cell lymphoma, Non-Hodgkin's lymphoma, Solid tumours
Product Property
- Purity
- > 95% as determined by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Applications
- Application Notes
- The TNFRSF9 antibody has been reported in applications of SPR, Activ, IF.
Target
- Alternative Names
- TNFRSF9; tumor necrosis factor receptor superfamily, member 9; ILA; 4-1BB; CD137; CDw137; tumor necrosis factor receptor superfamily member 9; CD137 antigen; T cell antigen ILA; T-cell antigen ILA; 4-1BB ligand receptor; homolog of mouse 4-1BB; receptor protein 4-1BB; T-cell antigen 4-1BB homolog; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63
- Gene ID
- 3604
- UniProt ID
- Q07011
Related Resources
Please refer to Utomilumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Utomilumab.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-179 | Anti-Human 4-1BB Recombinant Antibody (TAB-179) | ELISA, FC, IP, FuncS, IF, Neut, ICC | IgG4 - kappa |
Customer Reviews and Q&As
Customer Review
Q&As
Versatile and Reliable
We've used this antibody across various assays from flow cytometry to functional studies. It consistently delivers high-quality data that form the backbone of our immune response investigations, proving its versatility.
Boosts Cancer Research
Utilized extensively within our cancer immunotherapy projects, this antibody helps unravel TNFRSF9's role in enhancing anti-tumor immunity, offering promising directions for developing new therapeutic strategies.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-457CQ, RRID: AB_3111955)
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