Recombinant monoclonal antibody to TNFRSF9. This antibody is a human monoclonal antibody that specifically binds to the CD137 receptor (also called 4-1BB).
Figure 1 Anti-Human TNFRSF9 Recombinant Antibody (TAB-457CQ) in WB
Western blot analysis of TAB-457CQ was performed with Recombinant Human TNFRSF9 Protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-457CQ and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
Figure 2 Anti-Human TNFRSF9 Recombinant Antibody (TAB-457CQ) in ELISA
ELISA analysis of TAB-457CQ was performed by coating with Recombinant Human TNFRSF9 Protein. Then blocked with BSA, and incubated with Anti-Human TNFRSF9 Antibody (TAB-457CQ). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 3 Anti-Human TNFRSF9 Recombinant Antibody (TAB-457CQ)-Low endotoxin in SDS-PAGE
SDS-PAGE analysis of TAB-457CQ-LowE in β-mercaptoethanol-reduced (Lane 2) and non-reduced (Lane 1) conditions.
Figure 4 Anti-Human TNFRSF9 Recombinant Antibody (TAB-457CQ)-Low endotoxin in WB
Western blot analysis of TAB-457CQ-LowE was performed by loading human TNFRSF9 protein, His tag.
Figure 5 Anti-Human TNFRSF9 Recombinant Antibody (TAB-457CQ)-Low endotoxin in ELISA
ELISA analysis of TAB-457CQ-LowE was performed by coating with human TNFRSF9 protein (His tag). Then blocked with BSA and incubated with TAB-457CQ-LowE. The HRP conjugated Anti-human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
TAB-179 | Anti-Human 4-1BB Recombinant Antibody (TAB-179) | ELISA, FC, IP, FuncS, IF, Neut, ICC | IgG4 - kappa |
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(Creative Biolabs Cat# TAB-457CQ, RRID: AB_3111955)
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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