Rabbit Anti-APC Recombinant Antibody (clone 5F2) (CAT#: ZG-0545J)
This product is a rabbit antibody that recognizes APC.
Specific Inquiry
WB
Figure 1 Rabbit Anti-APC Antibody (ZG-0545J) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, SH-SY5Y whole cell lysate, A549 whole cell lysate, Rat Brain whole cell lysate
All lanes: APC antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 312, 301, 309 kDa
Observed band size: 160 kDa
IHC
Figure 2 Rabbit Anti-APC Antibody (ZG-0545J) in IHC
IHC image of ZG-0545J diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human APC
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human APC
- Species Reactivity
- Human, Rat
- Clone
- 5F2
- Applications
- ELISA, WB, IHC
- Conjugate
- Uconjugated
- Related Disease
- Cancer
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- GS; DP2; DP3; BTPS2; DESMD; DP2.5; PPP1R46; adenomatous polyposis coli protein; APC, WNT signaling pathway regulator; WNT signaling pathway regulator; adenomatosis polyposis coli tumor suppressor; adenomatous polyposis coli (APC); deleted in polyposis 2.5; epididymis secretory sperm binding protein; protein phosphatase 1, regulatory subunit 46; truncated adenomatosis polyposis coli
- Gene ID
- 324
- UniProt ID
- P25054
Related Resources
Breast Cancer
Colorectal Cancer
Endometrial Cancer
Gastric Cancer
Pancreatic Cancer
Prostate Cancer
Throid Cancer
Hepatocellular Carcinoma
Cushing Syndrome
Canonical Wnt Signaling Pathway
JAK-STAT Signaling Pathway
Non-Canonical Wnt Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "APC"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-72 | Recombinant Anti-human APC Antibody | ELISA, WB, FC, FuncS | IgG |
| MHH-72 | Recombinant Human Anti-human APC Antibody | WB, FACS, FuncS | IgG |
| MOB-0647MZ | Mouse Anti-APC Recombinant Antibody (clone CBLS-110) | IHC-Fr | Mouse IgG1 |
| MRO-1651-CN | Rabbit Anti-APC Polyclonal Antibody (MRO-1651-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-CJ816 | Rabbit Anti-APC Recombinant Antibody (VS3-CJ816) | WB, ICC, IP | Rabbit IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-72-F(E) | Recombinant Anti-human APC Antibody Fab Fragment | ELISA, WB, FuncS | Fab |
| MHH-72-F(E) | Recombinant Human Anti-human APC Antibody Fab Fragment | FC, Neut, Biosensors, FuncS | Fab |
| HPAB-0095-WJ-F(E) | Human Anti-APC Recombinant Antibody; Fab Fragment (HPAB-0095-WJ-F(E)) | ELISA | Humanized Fab |
| HPAB-J0036-YC-F(E) | Mouse Anti-aPC Recombinant Antibody; Fab Fragment (HPAB-J0036-YC-F(E)) | ELISA | Mouse Fab |
| HPAB-J0037-YC-F(E) | Mouse Anti-aPC Recombinant Antibody; Fab Fragment (HPAB-J0037-YC-F(E)) | ELISA | Mouse Fab |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-241CT-S(P) | Mouse Anti-aPC Recombinant Antibody; scFv Fragment (TAB-241CT-S(P)) | Non-neutralizing | Mouse scFv |
| TAB-242CT-S(P) | Human Anti-aPC Recombinant Antibody; scFv Fragment (TAB-242CT-S(P)) | ELISA, WB | Human scFv |
| TAB-243CT-S(P) | Human Anti-aPC Recombinant Antibody; scFv Fragment (TAB-243CT-S(P)) | ELISA, WB | Human scFv |
| TAB-244CT-S(P) | Human Anti-aPC Recombinant Antibody; scFv Fragment (TAB-244CT-S(P)) | ELISA, WB | Human scFv |
| TAB-245CT-S(P) | Human Anti-aPC Recombinant Antibody; scFv Fragment (TAB-245CT-S(P)) | ELISA, WB | Human scFv |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0199 | Hi-Affi™ Rabbit Anti-APC Recombinant Antibody (clone DS199AB) | WB, IHC, ICC | Rabbit IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0095-WJ-S(P) | Human Anti-APC Recombinant Antibody; scFv Fragment (HPAB-0095-WJ-S(P)) | ELISA | Humanized scFv |
| HPAB-J0036-YC-S(P) | Mouse Anti-aPC Recombinant Antibody; scFv Fragment (HPAB-J0036-YC-S(P)) | ELISA | Mouse scFv |
| HPAB-J0037-YC-S(P) | Mouse Anti-aPC Recombinant Antibody; scFv Fragment (HPAB-J0037-YC-S(P)) | ELISA | Mouse scFv |
| HPAB-J0038-YC-S(P) | Mouse Anti-aPC Recombinant Antibody; scFv Fragment (HPAB-J0038-YC-S(P)) | ELISA | Mouse scFv |
| HPAB-J0039-YC-S(P) | Mouse Anti-aPC Recombinant Antibody; scFv Fragment (HPAB-J0039-YC-S(P)) | ELISA | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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