Rabbit Anti-APC Recombinant Antibody (clone 5F2)
CAT#: ZG-0545J
This product is a rabbit antibody that recognizes APC.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-APC Antibody (ZG-0545J) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, SH-SY5Y whole cell lysate, A549 whole cell lysate, Rat Brain whole cell lysate
All lanes: APC antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 312, 301, 309 kDa
Observed band size: 160 kDa
IHC
Figure 2 Rabbit Anti-APC Antibody (ZG-0545J) in IHC
IHC image of ZG-0545J diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Subcellular Location
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to plasma membrane & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13362/1401_C6_5_selected.jpg
Normal Tissue
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong positivity in neuropil.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13349/165147_B_9_5_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuropil
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13349/165147_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13349/165147_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13349/165147_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Collecting ducts
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Distal tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Proximal tubules (cell body)
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13349/165147_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13349/165147_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134982-APC
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Specifications
- Immunogen
- A synthesized peptide derived from human APC
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human APC
- Species Reactivity
- Human, Rat
- Clone
- 5F2
- Applications
- ELISA, WB, IHC
- Conjugate
- Uconjugated
- Related Disease
- Cancer
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- GS; DP2; DP3; BTPS2; DESMD; DP2.5; PPP1R46; adenomatous polyposis coli protein; APC, WNT signaling pathway regulator; WNT signaling pathway regulator; adenomatosis polyposis coli tumor suppressor; adenomatous polyposis coli (APC); deleted in polyposis 2.5; epididymis secretory sperm binding protein; protein phosphatase 1, regulatory subunit 46; truncated adenomatosis polyposis coli
- Gene ID
- 324
- UniProt ID
- P25054
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Related Diseases
Breast Cancer
Colorectal Cancer
Endometrial Cancer
Gastric Cancer
Pancreatic Cancer
Prostate Cancer
Throid Cancer
Hepatocellular Carcinoma
Cushing Syndrome
Related Signaling Pathways
Canonical Wnt Signaling Pathway
JAK-STAT Signaling Pathway
Non-Canonical Wnt Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| MOB-72-S(P) | Recombinant Anti-human APC Antibody scFv Fragment | IP, IF, Biosensors, FuncS | scFv |
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| HPAB-J0037-YC-S(P) | Mouse Anti-aPC Recombinant Antibody; scFv Fragment (HPAB-J0037-YC-S(P)) | ELISA | Mouse scFv |
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|---|---|---|---|
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| TAB-243CT-F(E) | Human Anti-aPC Recombinant Antibody; Fab Fragment (TAB-243CT-F(E)) | ELISA, WB | Human Fab |
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| TAB-245CT-F(E) | Human Anti-aPC Recombinant Antibody; Fab Fragment (TAB-245CT-F(E)) | ELISA, WB | Human Fab |
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| VS-0525-XY439 | Anti-Mouse APC Immunohistochemistry Kit | IHC | |
| VS-0525-XY438 | Anti-Human APC Immunohistochemistry Kit | IHC |
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