Mouse Anti-CA9 Recombinant Antibody (clone 12F10) (CAT#: ZG-0057J)
This product is a mouse antibody that recognizes CA9.
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WB
Figure 1 Mouse Anti-CA9 Recombinant Antibody (clone 12F10) in WB
Western blot analysis of lysates from 1) Hela, 2) 293T cells, (Green)primary Antibody was diluted at 1:1000, 4°Cover night, Dylight 800 secondary Antibody was diluted at 1:10000, 37°C 1 hour. (Red)Tubulin β Polyclonal Antibody Antibody was diluted at 1:5000 as loading control, 4°Cover night, Dylight 680 secondary Antibody was diluted at 1:10000, 37°C1 hour.
IF
Figure 2 Mouse Anti-CA9 Recombinant Antibody (clone 12F10) in IF
Immunofluorescence analysis of human-liver-cancer tissue. 1.CA IX Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary Antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI.Picture C: merge of A+B
IHC
Figure 3 Mouse Anti-CA9 Recombinant Antibody (clone 12F10) in IHC
Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1.CA IX Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min). 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary Antibody only.
WB
Figure 4 Mouse Anti-CA9 Recombinant Antibody (clone 12F10) in WB
Western blot analysis of 1) Hela, 2) 293T, diluted at 1:5000. Cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.
IHC
Figure 5 Mouse Anti-CA9 Recombinant Antibody (clone 12F10) in IHC
Immunohistochemical analysis of paraffin-embedded Human gallbladder. Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min).
Specifications
- Immunogen
- Synthetic Peptide of CA IX
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human CA9
- Species Reactivity
- Human
- Clone
- 12F10
- Applications
- IF/ICC, WB, IHC-P, IP
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Immunofluorescence/Immunocytochemistry, Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation.
Target
- Alternative Names
- MN; CAIX; carbonic anhydrase 9; CA-IX; P54/58N; RCC-associated antigen G250; RCC-associated protein G250; carbonate dehydratase IX; carbonic anhydrase IX; carbonic dehydratase; membrane antigen MN; pMW1; renal cell carcinoma-associated antigen G250
- Gene ID
- 768
- UniProt ID
- Q16790
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-707 | Anti-CAIX/CA9 Recombinant Antibody (Girentuximab) | WB, FuncS, IF, Neut, ELISA, FC, IP | IgG1 - kappa |
| TAB-H43 | Anti-Human CA9 Recombinant Antibody (TAB-H43) | ELISA, IP, IF, Inhib | IgG1 - kappa |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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