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    Mouse Anti-CD276 Recombinant Antibody (HPAB-0048LY) (CAT#: HPAB-0048LY)

    This product is a mouse-derived IgG monoclonal antibody expressed by hybridoma cells, specifically targeting the human B7-H3 protein. The anti-B7-H3 antibody recognizes cancer stem cell lines, regulates angiogenic factor secretion, and exhibits potent antitumor activity in vivo. HPAB-0048LY significantly reduced VEGF secretion by up to 60% in the subset of cancer stem cell lines examined.

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    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
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    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line A-431
    Figure 1 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to vesicles.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/17139/if_selected.jpg
    Normal Tissue
    Figure 2 Placenta.
    Figure 2 Placenta.
    (High expression in placenta)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/17826/40943_A_1_7_val_selected.jpg
    Figure 3 Testis
    Figure 3 Testis
    (Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/9285/25731_A_5_6.jpg
    Figure 4 Cerebral cortex
    Figure 4 Cerebral cortex
    (Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/9285/25731_B_7_5.jpg
    Figure 5 Colon
    Figure 5 Colon
    (Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/9285/25731_A_9_3.jpg
    Figure 6 Liver
    Figure 6 Liver
    (Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/9285/25731_A_7_4.jpg
    RNA Expression
    Figure 7 RNA cell line category: Cell line enhanced (HaCaT, U-2197)
    Figure 7 RNA cell line category: Cell line enhanced (HaCaT, U-2197)
    (Cell lines ordered by descending RNA expression order)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000103855-CD276

    Specifications

    • Immunogen
    • Human fetal testis cells as an immunogen.
    • Host Species
    • Mouse
    • Type
    • Mouse IgG
    • Specificity
    • The antibody is binding to the IgV1-IgC1 portion of B7-H3.
    • Species Reactivity
    • Human
    • Applications
    • FuncS, ELISA, FC, IHC

    Product Property

    • Purity
    • >95% as determined by SDS-PAGE
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Buffer
    • PBS
    • Preservative
    • No preservatives
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

    Applications

    • Application Notes
    • Immunohistochemistry: 1.25 μg/mL-5 μg/mL

    Target

    • Alternative Names
    • CD276 Molecule; CD276 Antigen; Costimulatory Molecule; B7 Homolog 3; 4Ig-B7-H3; B7-H3; B7H3; B7RP-2;

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "CD276"

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    CAT Product Name Application Type
    TAB-117CL Anti-Human CD276 Recombinant Antibody (TAB-117CL) ADCC, FuncS Antibody
    TAB-424CQ Anti-Human CD276 Recombinant Antibody (TAB-424CQ) ELISA, IHC, FC, IP, IF, FuncS IgG1, κ

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