Mouse Anti-CD276 Recombinant Antibody (VS3-FY2653)
CAT#: VS3-FY2653
The anti-CD276 antibody is a mouse recombinant monoclonal antibody with high affinity for CD276. The calculated molecular weight of the antigen is 57.2 kDa. It shows capability of cross-reacting with human, mouse and rat. This monoclonal antibody can be used in Western Blot, Immunohistochemistry. This monoclonal antibody is useful for conducting research on cancer.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17139/if_selected.jpg
Normal Tissue
Figure 2 Placenta.
High expression in placenta
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17826/40943_A_1_7_val_selected.jpg
Normal Tissue
Figure 3 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9285/25731_A_5_6.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9285/25731_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9285/25731_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9285/25731_A_7_4.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HaCaT, U-2197)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000103855-CD276
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- CD276 protein of human, mouse and rat origins
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 57.2 kDa.
- Related Disease
- Cancer
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunohistochemistry: 1/50-1/200
Target
- Alternative Names
- CD276 Molecule; CD276 Antigen; Costimulatory Molecule; B7 Homolog 3; 4Ig-B7-H3; B7-H3; B7H3; B7RP-2
- Gene ID
- 80381
- UniProt ID
- Q5ZPR3
- Sequence Similarities
- Belongs to the immunoglobulin superfamily. BTN/MOG family.
- Cellular Localization
- Membrane
- Post Translation Modifications
- Glycosylation at Asn91, Asn104, Asn189, Asn407, Asn215, Asn433, and Asn322
Modification sites at PhosphoSitePlus
Glycosylation from Gly
Glycosylation from GlyGen 9 sites, 37 N-linked glycans (3 sites), 1 O-linked glycan (2 sites)
- Protein Refseq
- XP_005254757.1; XP_011520397.1; XP_016878127.1; XP_047289103.1; XP_047289104.1
- Function
- May participate in the regulation of T-cell-mediated immune response.
May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells.
May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "CD276"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-117CL | Anti-Human CD276 Recombinant Antibody (TAB-117CL) | ADCC, FuncS | Antibody |
| TAB-424CQ | Anti-Human CD276 Recombinant Antibody (TAB-424CQ) | ELISA, IHC, FC, IP, IF, FuncS | IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-333CQ | Rat Anti-CD276 Recombinant Antibody (clone MJ18) | Block, FC | Rat IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0549 | Hi-Affi™ Rabbit Anti-CD276 Recombinant Antibody (clone DS549AB) | WB, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0234-CN | Human Anti-CD276 Recombinant Antibody (HPAB-0234-CN) | ELISA, FC, IF | Human IgG |
| HPAB-0236-CN | Mouse Anti-CD276 Recombinant Antibody (HPAB-0236-CN) | IHC, Cyt, Inhib, ELISA, FC | Mouse IgG1, κ |
| HPAB-0237-CN | Mouse Anti-CD276 Recombinant Antibody (HPAB-0237-CN) | IHC, ELISA, FC | Mouse IgG1, κ |
| HPAB-0048LY | Mouse Anti-CD276 Recombinant Antibody (HPAB-0048LY) | FuncS, ELISA, FC, IHC | Mouse IgG |
| HPAB-0049LY | Human Anti-CD276 Recombinant Antibody (HPAB-0049LY) | FuncS, ELISA, FC, IHC | Humanized IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0048LY-F(E) | Mouse Anti-CD276 Recombinant Antibody; Fab Fragment (HPAB-0048LY-F(E)) | FuncS, ELISA, FC, IHC | Mouse Fab |
| HPAB-0049LY-F(E) | Human Anti-CD276 Recombinant Antibody; Fab Fragment (HPAB-0049LY-F(E)) | FuncS, ELISA, FC, IHC | Humanized Fab |
| HPAB-M0422-YC-F(E) | Mouse Anti-CD276 Recombinant Antibody; Fab Fragment (HPAB-M0422-YC-F(E)) | Cyt, FuncS | Mouse Fab |
| HPAB-M0423-YC-F(E) | Mouse Anti-CD276 Recombinant Antibody; Fab Fragment (HPAB-M0423-YC-F(E)) | Cyt, FuncS | Mouse Fab |
| HPAB-M0424-YC-F(E) | Human Anti-CD276 Recombinant Antibody; Fab Fragment (HPAB-M0424-YC-F(E)) | Cyt, FuncS, IHC | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0422-YC-S(P) | Mouse Anti-CD276 Recombinant Antibody; scFv Fragment (HPAB-M0422-YC-S(P)) | Cyt, FuncS | Mouse scFv |
| HPAB-M0423-YC-S(P) | Mouse Anti-CD276 Recombinant Antibody; scFv Fragment (HPAB-M0423-YC-S(P)) | Cyt, FuncS | Mouse scFv |
| HPAB-M0424-YC-S(P) | Human Anti-CD276 Recombinant Antibody; scFv Fragment (HPAB-M0424-YC-S(P)) | Cyt, FuncS, IHC | Human scFv |
| HPAB-M0425-YC-S(P) | Mouse Anti-CD276 Recombinant Antibody; scFv Fragment (HPAB-M0425-YC-S(P)) | Cyt, FuncS | Mouse scFv |
| HPAB-M0426-YC-S(P) | Human Anti-CD276 Recombinant Antibody; scFv Fragment (HPAB-M0426-YC-S(P)) | Cyt, FuncS, IHC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-424CQ | Afuco™ Anti-CD276 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-424CQ) | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1279 | AbPlus™ Anti-Cd276 Magnetic Beads (VS-0724-YC1279) | IP, Protein Purification | |
| VS-0724-YC1280 | AbPlus™ Anti-Cd276 Magnetic Beads (VS-0724-YC1280) | IP, Protein Purification | |
| VS-0724-YC1366 | AbPlus™ Anti-CD276 Magnetic Beads (VS-0724-YC1366) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY67 | Human Anti-CD276 (clone MGA271) scFv-Fc Chimera | ADCC | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC156 | Recombinant Anti-CD276 Vesicular Antibody, EV Displayed (VS-0425-YC156) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1155 | Anti-CD276 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1156 | Anti-Mouse CD276 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0825-YC48 | SmartAb™ Recombinant Anti-CD276 pH-dependent Antibody (VS-0825-YC48) | ELISA, IHC, FC, IP, IF | Human IgG1 kappa |
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