Recombinant Mouse Anti-CTLA4 Antibody (HK44) (CAT#: HPAB-0478LY)
This product is antibody recognizes CTLA4. The antibody HK44 immunoassay techniques such as: WB, FITC.
Specific Inquiry
IF
Figure 1 Specific reactions of the mAbs HK24 and HK44 with epitope-76.
Fluorescence microscopic images of cells. Green fluorescence was only observed on cells transfected with the HK24 or HK44 expression vectors.
Dong, H., Su, A., Lv, D., Ma, L., Dong, J., Guo, N., ... & Ouyan, H. (2019). Development of Whole-Porcine Monoclonal Antibodies with Potent Neutralization Activity against Classical Swine Fever Virus from Single B Cells. ACS synthetic biology, 8(5), 989-1000.
FC
Figure 2 Specific reactions of the mAbs HK24 and HK44 with epitope-76.
Analysis of the affinity of HK24 and HK44 for epitope-76. Affinity was analysed using FACS. NC is cells transfected with empty vectors.
Dong, H., Su, A., Lv, D., Ma, L., Dong, J., Guo, N., ... & Ouyan, H. (2019). Development of Whole-Porcine Monoclonal Antibodies with Potent Neutralization Activity against Classical Swine Fever Virus from Single B Cells. ACS synthetic biology, 8(5), 989-1000.
ELISA
Figure 3 Specific reactions of the mAbs HK24 and HK44 with epitope-76.
Antibody specificity against epitope-76 was measured via a fluorescence assay.
Dong, H., Su, A., Lv, D., Ma, L., Dong, J., Guo, N., ... & Ouyan, H. (2019). Development of Whole-Porcine Monoclonal Antibodies with Potent Neutralization Activity against Classical Swine Fever Virus from Single B Cells. ACS synthetic biology, 8(5), 989-1000.
WB
Figure 4 The mAbs HK24 and HK44 bound to the E2 protein.
Recombinant CSFV E2 protein (690-866 aa) expressed in a bacterial system was recognized by the mAbs HK24 and HK44 and determined via Western blotting.
Dong, H., Su, A., Lv, D., Ma, L., Dong, J., Guo, N., ... & Ouyan, H. (2019). Development of Whole-Porcine Monoclonal Antibodies with Potent Neutralization Activity against Classical Swine Fever Virus from Single B Cells. ACS synthetic biology, 8(5), 989-1000.
SPR
Figure 5 MAbs HK44 and HK24 bound to recombinant E2 protein.
Binding of E2 protein at concentrations of 2000, 1000, 500, 250 and 125 nM to mAbs MAb HK24.
Dong, H., Su, A., Lv, D., Ma, L., Dong, J., Guo, N., ... & Ouyan, H. (2019). Development of Whole-Porcine Monoclonal Antibodies with Potent Neutralization Activity against Classical Swine Fever Virus from Single B Cells. ACS synthetic biology, 8(5), 989-1000.
Neut
Figure 6 The mAbs HK24 and HK44 neutralize CSFV.
The mAbs HK24 and HK44 were tested for neutralization reactivity with CSFV via the IFA neutralization assay.
Dong, H., Su, A., Lv, D., Ma, L., Dong, J., Guo, N., ... & Ouyan, H. (2019). Development of Whole-Porcine Monoclonal Antibodies with Potent Neutralization Activity against Classical Swine Fever Virus from Single B Cells. ACS synthetic biology, 8(5), 989-1000.
Specifications
- Host Species
- Mouse
- Specificity
- Human
- Species Reactivity
- porcine marks virus
- Clone
- HK44
- Applications
- WB, IF, FC, ELISA, SPR, Neut
- Related Disease
- Cytotoxic T-Lymphocyte Associated Protein 4
Product Property
- Purification
- 95% pure by SDS-PAGE
- Purity
- > 95% Purity determined by SDS-PAGE.
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
Applications
- Application Notes
- The CTLA4 antibody has been reported in applications of Western Blot, Immunofluorescence, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Surface Plasmon Resonance, Neutralization.
Target
- Alternative Names
- Cytotoxic T-Lymphocyte Associated Protein 4; Insulin-Dependent Diabetes Mellitus 12; Celiac Disease 3; CTLA-4; CD152; Ligand And Transmembrane Spliced Cytotoxic T Lymphocyte Associated Antigen 4; Cytotoxic T Lymphocyte Associated Antigen 4 Short Spliced Form; Cytotoxic T-Lymphocyte-Associated Serine Esterase-4; Cytotoxic T-Lymphocyte-Associated Antigen 4; Cytotoxic T-Lymphocyte Protein 4;
- Gene ID
- 1493
- UniProt ID
- P16410
Related Resources
Rheumatoid Arthritis
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone HK44"
See other products for "CTLA4"
Select a product category from the dropdown menu below to view related products.
Please select product type
Human Antibody
Immunotoxin
Rat Antibody
Humanized Antibody
Chimeric Antibody
Mouse Antibody
Neutralizing Antibody
Blocking Antibody
Rabbit Monoclonal Antibody
Recombinant Antibody
scFv Fragment Antibody
Fab Fragment Antibody
ADCC Enhanced Antibody
Single-domain Antibody
Agonistic Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-067 | Anti-Human CTLA4 Recombinant Antibody (Ipilimumab) | WB, FuncS, IF, Neut, ELISA, FC, IP | IgG1 - kappa |
| TAB-206 | Anti-Human CTLA4 Recombinant Antibody (Ticilimumab (Tremelimumab)) | WB, IF, IP, Neut, FuncS, ELISA, FC | IgG2 |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for HPAB-0478LY. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: WB, ELISA, IP, FC, FuncS, Neut, IF
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, IHC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: ELISA, WB, BLI, SPR
Application: WB, ELISA, Neut, FuncS
Application: ELISA, Inhib, FuncS
Application: ELISA, WB, Microarray, Block
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
