Recombinant Mouse Anti-CTLA4 Antibody (HK44) (CAT#: HPAB-0478LY)
This product is antibody recognizes CTLA4. The antibody HK44 immunoassay techniques such as: WB, FITC.
Specific Inquiry
IF
Figure 1 Specific reactions of the mAbs HK24 and HK44 with epitope-76.
Fluorescence microscopic images of cells. Green fluorescence was only observed on cells transfected with the HK24 or HK44 expression vectors.
Dong, H., Su, A., Lv, D., Ma, L., Dong, J., Guo, N., ... & Ouyan, H. (2019). Development of Whole-Porcine Monoclonal Antibodies with Potent Neutralization Activity against Classical Swine Fever Virus from Single B Cells. ACS synthetic biology, 8(5), 989-1000.
FC
Figure 2 Specific reactions of the mAbs HK24 and HK44 with epitope-76.
Analysis of the affinity of HK24 and HK44 for epitope-76. Affinity was analysed using FACS. NC is cells transfected with empty vectors.
Dong, H., Su, A., Lv, D., Ma, L., Dong, J., Guo, N., ... & Ouyan, H. (2019). Development of Whole-Porcine Monoclonal Antibodies with Potent Neutralization Activity against Classical Swine Fever Virus from Single B Cells. ACS synthetic biology, 8(5), 989-1000.
ELISA
Figure 3 Specific reactions of the mAbs HK24 and HK44 with epitope-76.
Antibody specificity against epitope-76 was measured via a fluorescence assay.
Dong, H., Su, A., Lv, D., Ma, L., Dong, J., Guo, N., ... & Ouyan, H. (2019). Development of Whole-Porcine Monoclonal Antibodies with Potent Neutralization Activity against Classical Swine Fever Virus from Single B Cells. ACS synthetic biology, 8(5), 989-1000.
WB
Figure 4 The mAbs HK24 and HK44 bound to the E2 protein.
Recombinant CSFV E2 protein (690-866 aa) expressed in a bacterial system was recognized by the mAbs HK24 and HK44 and determined via Western blotting.
Dong, H., Su, A., Lv, D., Ma, L., Dong, J., Guo, N., ... & Ouyan, H. (2019). Development of Whole-Porcine Monoclonal Antibodies with Potent Neutralization Activity against Classical Swine Fever Virus from Single B Cells. ACS synthetic biology, 8(5), 989-1000.
SPR
Figure 5 MAbs HK44 and HK24 bound to recombinant E2 protein.
Binding of E2 protein at concentrations of 2000, 1000, 500, 250 and 125 nM to mAbs MAb HK24.
Dong, H., Su, A., Lv, D., Ma, L., Dong, J., Guo, N., ... & Ouyan, H. (2019). Development of Whole-Porcine Monoclonal Antibodies with Potent Neutralization Activity against Classical Swine Fever Virus from Single B Cells. ACS synthetic biology, 8(5), 989-1000.
Neut
Figure 6 The mAbs HK24 and HK44 neutralize CSFV.
The mAbs HK24 and HK44 were tested for neutralization reactivity with CSFV via the IFA neutralization assay.
Dong, H., Su, A., Lv, D., Ma, L., Dong, J., Guo, N., ... & Ouyan, H. (2019). Development of Whole-Porcine Monoclonal Antibodies with Potent Neutralization Activity against Classical Swine Fever Virus from Single B Cells. ACS synthetic biology, 8(5), 989-1000.
Specifications
- Host Species
- Mouse
- Specificity
- Human
- Species Reactivity
- porcine marks virus
- Clone
- HK44
- Applications
- WB, IF, FC, ELISA, SPR, Neut
- Related Disease
- Cytotoxic T-Lymphocyte Associated Protein 4
Product Property
- Purification
- 95% pure by SDS-PAGE
- Purity
- > 95% Purity determined by SDS-PAGE.
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
Applications
- Application Notes
- The CTLA4 antibody has been reported in applications of Western Blot, Immunofluorescence, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Surface Plasmon Resonance, Neutralization.
Target
- Alternative Names
- Cytotoxic T-Lymphocyte Associated Protein 4; Insulin-Dependent Diabetes Mellitus 12; Celiac Disease 3; CTLA-4; CD152; Ligand And Transmembrane Spliced Cytotoxic T Lymphocyte Associated Antigen 4; Cytotoxic T Lymphocyte Associated Antigen 4 Short Spliced Form; Cytotoxic T-Lymphocyte-Associated Serine Esterase-4; Cytotoxic T-Lymphocyte-Associated Antigen 4; Cytotoxic T-Lymphocyte Protein 4;
- Gene ID
- 1493
- UniProt ID
- P16410
Related Resources
Rheumatoid Arthritis
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone HK44"
See other products for "CTLA4"
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-067 | Anti-Human CTLA4 Recombinant Antibody (Ipilimumab) | WB, FuncS, IF, Neut, ELISA, FC, IP | IgG1 - kappa |
| TAB-206 | Anti-Human CTLA4 Recombinant Antibody (Ticilimumab (Tremelimumab)) | WB, IF, IP, Neut, FuncS, ELISA, FC | IgG2 |
Immunotoxin
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G015E | Anti-CTLA4 immunotoxin (scFv)-PE | Cytotoxicity assay, Function study | |
| AGTO-G015S | Anti-CTLA4 immunotoxin (scFv)-Sap | Cytotoxicity assay, Function study | |
| AGTO-G015M | Anti-CTLA4 immunotoxin (scFv)-Mel | Cytotoxicity assay, Function study |
Rat Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-195CL | Mouse Anti-CTLA4 Recombinant Antibody (TAB-195CL) | ELISA | Mouse IgG |
Chimeric Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-085MZ | Anti-Human CTLA4 Recombinant Antibody (TGN2122.C) | ELISA, FC | Chimeric antibody (mouse/human) |
| TAB-086MZ | Anti-Human CTLA4 Recombinant Antibody (TGN2422.C) | ELISA, FC | Chimeric antibody (mouse/human) |
| TAB-085MZ-S(P) | Anti-Human CTLA4 Recombinant Antibody scFv Fragment (TGN2122.C) | ELISA, FC | Chimeric antibody (mouse/human) |
| TAB-086MZ-S(P) | Anti-Human CTLA4 Recombinant Antibody scFv Fragment (TGN2422.C) | ELISA, FC | Chimeric antibody (mouse/human) |
| TAB-085MZ-F(E) | Anti-Human CTLA4 Recombinant Antibody Fab Fragment (TGN2122.C) | ELISA, FC | Chimeric antibody (mouse/human) |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-087MZ-S(P) | Mouse Anti-CTLA4 Recombinant Antibody; scFv Fragment (TAB-087MZ-S(P)) | ELISA | Mouse scFv |
| TAB-089MZ-S(P) | Mouse Anti-CTLA4 Recombinant Antibody; scFv Fragment (TAB-089MZ-S(P)) | ELISA | Mouse scFv |
| TAB-090MZ-S(P) | Mouse Anti-CTLA4 Recombinant Antibody; scFv Fragment (TAB-090MZ-S(P)) | ELISA | Mouse scFv |
| TAB-093MZ-S(P) | Anti-Human CTLA4 Recombinant Antibody scFv Fragment (Mu-26B) | ELISA | |
| MOB-0317MC | Recombinant Mouse Anti-CTLA4 Antibody | ELISA, FACS |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-083MZ-F(E) | Anti-Human CTLA4 Recombinant Antibody Fab Fragment (TGN2122.H) | ELISA, FC | Humanized antibody |
| TAB-084MZ-F(E) | Anti-Human CTLA4 Recombinant Antibody Fab Fragment (TGN2422.H) | ELISA, FC | Humanized antibody |
| TAB-088MZ-F(E) | Human Anti-CTLA4 Recombinant Antibody; Fab Fragment (TAB-088MZ-F(E)) | ELISA | Humanized Fab |
| TAB-091MZ-F(E) | Human Anti-CTLA4 Recombinant Antibody; Fab Fragment (TAB-091MZ-F(E)) | ELISA | Humanized Fab |
| TAB-092MZ-F(E) | Human Anti-CTLA4 Recombinant Antibody; Fab Fragment (TAB-092MZ-F(E)) | ELISA | Humanized Fab |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-567CQ | Mouse Anti-CTLA4 Recombinant Antibody (clone 14D3) | FC, FuncS, Neut, IP, WB | Mouse IgG2a, κ |
| NEUT-569CQ | Mouse Anti-CTLA4 Recombinant Antibody (clone AS32) | WB, Neut | Mouse IgG1 |
| NEUT-570CQ | Human Anti-CTLA4 Recombinant Antibody (clone YC030) | Neut | Human IgG1, κ |
| NEUT-572CQ | Hamster Anti-Ctla4 Recombinant Antibody (clone UC10-4B9) | FC, Block, ELISA, IP | Hamster IgG |
| NEUT-573CQ | Rat Anti-Ctla4 Recombinant Antibody (clone 63828) | WB, FC, Neut | Rat IgG2a |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-568CQ | Mouse Anti-CTLA4 Recombinant Antibody (clone L3D10) | FC, Block | Mouse IgG1, κ |
| NEUT-571CQ | Mouse Anti-CTLA4 Recombinant Antibody (clone BN13) | Block, FC | Mouse IgG2a |
| NEUT-577CQ | Hamster Anti-Ctla4 Recombinant Antibody (clone MAB0835) | Block, FC | Hamster IgG |
| NEUT-578CQ | Hamster Anti-Ctla4 Recombinant Antibody (clone RYNmCTLA-4) | Block | Hamster IgG |
| NEUT-579CQ | Hamster Anti-Ctla4 Recombinant Antibody (clone 9H10) | FC, Block | Hamster IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0855 | Hi-Affi™ Rabbit Anti-CTLA4 Recombinant Antibody (clone DS855AB) | WB | Rabbit IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0157-FY-F(E) | Human Anti-CTLA4 Recombinant Antibody; Fab Fragment (HPAB-0157-FY-F(E)) | Activ | Humanized Fab |
| HPAB-0749LY-F(E) | Human Anti-CTLA4 Recombinant Antibody; Fab Fragment (HPAB-0749LY-F(E)) | FuncS, ELISA | Human Fab |
| HPAB-0750LY-F(E) | Human Anti-CTLA4 Recombinant Antibody; Fab Fragment (HPAB-0750LY-F(E)) | FuncS, ELISA | Human Fab |
| HPAB-0751LY-F(E) | Human Anti-CTLA4 Recombinant Antibody; Fab Fragment (HPAB-0751LY-F(E)) | FuncS, ELISA | Human Fab |
| HPAB-0752LY-F(E) | Human Anti-CTLA4 Recombinant Antibody; Fab Fragment (HPAB-0752LY-F(E)) | FuncS, ELISA | Human Fab |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-610-FY | Human Anti-CTLA4 Recombinant Antibody (HPAB-610-FY) | ELISA | Human IgG |
| HPAB-611-FY | Human Anti-CTLA4 Recombinant Antibody (HPAB-611-FY) | ELISA | Human IgG |
| FN-003CQ | Mouse Anti-CTLA4 Recombinant Antibody (clone ANC152.2/8H5) | ELISA, FC, Block | Mouse IgG1, κ |
| HPAB-2088LY | Human Anti-CTLA4 Recombinant Antibody (HPAB-2088LY) | ELISA, FC | Human IgG |
| HPAB-0219-WJ | Human Anti-CTLA4 Recombinant Antibody (HPAB-0219-WJ) | ELISA, WB, FC | Human IgG1 |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-067 | Afuco™ Anti-CTLA4 ADCC Recombinant Antibody (Ipilimumab), ADCC Enhanced | FuncS, IF, Neut, ELISA, FC | ADCC enhanced antibody |
| AFC-TAB-206 | Afuco™ Anti-CTLA4 ADCC Recombinant Antibody (Ticilimumab (= Tremelimumab)), ADCC Enhanced | IF, IP, Neut, FuncS, ELISA | ADCC enhanced antibody |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0041-YC-S(P) | Mouse Anti-CTLA4 Recombinant Antibody; scFv Fragment (HPAB-M0041-YC-S(P)) | ELISA, FC, FuncS | Mouse scFv |
| HPAB-M0042-YC-S(P) | Human Anti-CTLA4 Recombinant Antibody; scFv Fragment (HPAB-M0042-YC-S(P)) | ELISA, FC, FuncS | Human scFv |
| HPAB-M0043-YC-S(P) | Human Anti-CTLA4 Recombinant Antibody; scFv Fragment (HPAB-M0043-YC-S(P)) | ELISA, FC, FuncS | Human scFv |
| HPAB-M0044-YC-S(P) | Human Anti-CTLA4 Recombinant Antibody (clone F7); scFv Fragment | ELISA, FuncS | Human scFv |
| HPAB-M0045-YC-S(P) | Human Anti-CTLA4 Recombinant Antibody (clone E8); scFv Fragment | ELISA, FuncS | Human scFv |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP860-YC | Camelid Anti-CTLA4 Recombinant Antibody; VHH Fragment (HPAB-AP860-YC) | ELISA, Block | Camel VHH |
| HPAB-AP861-YC | Camelid Anti-CTLA4 Recombinant Antibody; VHH Fragment (HPAB-AP861-YC) | ELISA, Block | Camel VHH |
| HPAB-AP862-YC | Camelid Anti-CTLA4 Recombinant Antibody; VHH Fragment (HPAB-AP862-YC) | ELISA, Block | Camel VHH |
| HPAB-AP863-YC | Camelid Anti-CTLA4 Recombinant Antibody; VHH Fragment (HPAB-AP863-YC) | ELISA, Block | Camel VHH |
| HPAB-AP864-YC | Camelid Anti-CTLA4 Recombinant Antibody; VHH Fragment (HPAB-AP864-YC) | ELISA, Block | Camel VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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