Human Anti-CTLA4 Recombinant Antibody (clone 10D1) (CAT#: HPAB-0051-YC)

The human sequence antibody specifically binds to human CTLA-4 or the CTLA-4 on the cell surface of normal human T cell. Herein the antibody is useful for treating human diseases, infections and other conditions using these antibodies.


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  • Published Data
  • Gene Expression
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FC

Figure 1 shows melanoma-specific antibody responses in animals treated with a melanoma vaccine and the anti CTLA-4 antibody 10D1.

Figure 1 shows melanoma-specific antibody responses in animals treated with a melanoma vaccine and the anti CTLA-4 antibody 10D1.

Antibody reactivity in pooled plasma samples from treated animals was measured by flow cytometry.

FuncS

Figure 2 shows the pharmacokinetic profile of the anti CTLA-4 antibody 10D1 during chronic dosing of primates.

Figure 2 shows the pharmacokinetic profile of the anti CTLA-4 antibody 10D1 during chronic dosing of primates.

Antibody was administered on days 0, 28, 56.84 and 140 and plasma concentrations of 10D1 were analyzed by ELISA. The mean +/-SEM of six treated animals is shown.

FuncS

Figure 3 shows the pharmacokinetic profile of anti-CTLA-4 antibody 10D1 in prostate cancer patients treated with a single dose of 10D1 at day 0

Figure 3 shows the pharmacokinetic profile of anti-CTLA-4 antibody 10D1 in prostate cancer patients treated with a single dose of 10D1 at day 0

Plasma concentration (ug/ml) is shown. The mean +/-SEM for 14 treated patients is shown.

BL

Figure 4 Ligand blocking by mAb 10D1. Inhibition of recombinant B7.1 (A) and B7.2 (B) binding to CTLA-4-expressing T cells.

Figure 4 Ligand blocking by mAb 10D1. Inhibition of recombinant B7.1 (A) and B7.2 (B) binding to CTLA-4-expressing T cells.

B7-Ig fusion proteins were incubated with huCTLA4 transfectants in the presence of varying concentrations of 10D1 F(ab)2 or isotype control F(ab)2. The mouse anti-human CTLA-4 mAb BNI-3 was also included as a positive control. B7-Ig binding was detected using goat anti-human IgG (Fc-specific)-PE probe. The cell-associated fluorescence was determined by flow cytometry, and percent inhibition of binding was determined.

Davis, T., Lonberg, N., Korman, A., Blanset, S. L., Srinivasan, M., & Graziano, R. F. (2003). Activity and Safety of CTLA-4 Blockade. J Immunol, 171, 6251-6259.

BL

Figure 5 CTLA-4 blockade with mAb 10D1 enhances Ab response to a melanoma vaccine in monkeys

Figure 5 CTLA-4 blockade with mAb 10D1 enhances Ab response to a melanoma vaccine in monkeys

Groups of six cynomolgus monkeys were given s.c. injections of 5106 SKmel-GM cells alone (arrows) or in combination with a bolus i.v. administration of mAb 10D1 (10 mg/kg). Analysis of Ab responses to SKmel-3 cells by flow cytometry (A) or ELISA (B) was determined from plasma samples taken 2 wk after each immunization. The values represent the mean SE from plasma samples, 6/group, tested at a 1/1000 dilution for flow cytometry or 1/200 for ELISA. Statistically significant differences by the Student t test are noted; p= 0.05,p=0.01,p=0.005. C, The individual animal responses by flow cytometry or ELISA (pretreatment values subtracted) at day 97.

Davis, T., Lonberg, N., Korman, A., Blanset, S. L., Srinivasan, M., & Graziano, R. F. (2003). Activity and Safety of CTLA-4 Blockade. J Immunol, 171, 6251-6259.

FuncS

Figure 6 Ab responses to melanoma and nonmelanoma cell lines in animals treated with melanoma vaccine and mAb 10D1.

Figure 6 Ab responses to melanoma and nonmelanoma cell lines in animals treated with melanoma vaccine and mAb 10D1.

Pooled plasma from six cynomolgus monkeys given SKmel-3-GM-CSF vaccine and mAb 10D1 (day 41) was tested for reactivity to a variety of melanoma and nonmelanoma cell lines by flow cytometry (1/1000 dilution).p 0.05human melanoma cell binding vs human nonmelanoma cells.

Davis, T., Lonberg, N., Korman, A., Blanset, S. L., Srinivasan, M., & Graziano, R. F. (2003). Activity and Safety of CTLA-4 Blockade. J Immunol, 171, 6251-6259.

ADCC

Figure 7 CTLA-4 blockade enhances ADCC activity in response to the melanoma vaccine.

Figure 7 CTLA-4 blockade enhances ADCC activity in response to the melanoma vaccine.

Pooled plasma samples from vaccinated animals with or without 10D1 mAb were taken prior to immunization (Pre) or 2 wk after the second immunization (day 41). The samples were tested for ADCC activity on three melanoma cell lines at a 1/500 dilution.

Davis, T., Lonberg, N., Korman, A., Blanset, S. L., Srinivasan, M., & Graziano, R. F. (2003). Activity and Safety of CTLA-4 Blockade. J Immunol, 171, 6251-6259.


Specifications

  • Immunogen
  • Cells in PBS and soluble fusion protein
  • Host Species
  • Human
  • Type
  • Human IgG, κ
  • Specificity
  • Human CTLA4
  • Species Reactivity
  • Human, Monkey
  • Clone
  • 10D1
  • Applications
  • ELISA, Cyt, Block

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • Cytotoxic T-Lymphocyte-Associated Protein 4; Insulin-Dependent Diabetes Mellitus 12; Celiac Disease 3; CTLA-4; CD152; Ligand And Transmembrane Spliced Cytotoxic T Lymphocyte Associated Antigen 4; Cytotoxic T Lymphocyte Associated Antigen 4 Short Spliced Form; Cytotoxic T-Lymphocyte-Associated Serine Esterase-4; Cytotoxic T-Lymphocyte-Associated Antigen 4; CD152 Isoform; CD152 Antigen; CELIAC3; IDDM12; ALPS5; GRD4; GSE; CD

Related Resources

  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "Clone 10D1"

See other products for "CTLA4"

Human Antibody

CAT Product Name Application Type
TAB-067 Anti-Human CTLA4 Recombinant Antibody (Ipilimumab) WB, FuncS, IF, Neut, ELISA, FC, IP IgG1 - kappa
TAB-206 Anti-Human CTLA4 Recombinant Antibody (Ticilimumab (Tremelimumab)) WB, IF, IP, Neut, FuncS, ELISA, FC IgG2

Immunotoxin

CAT Product Name Application Type
AGTO-G015E Anti-CTLA4 immunotoxin (scFv)-PE Cytotoxicity assay, Function study
AGTO-G015S Anti-CTLA4 immunotoxin (scFv)-Sap Cytotoxicity assay, Function study
AGTO-G015M Anti-CTLA4 immunotoxin (scFv)-Mel Cytotoxicity assay, Function study

Rat Antibody

CAT Product Name Application Type
TAB-195CL Mouse Anti-CTLA4 Recombinant Antibody (TAB-195CL) ELISA Mouse IgG

Chimeric Antibody

CAT Product Name Application Type
TAB-085MZ Anti-Human CTLA4 Recombinant Antibody (TGN2122.C) ELISA, FC Chimeric antibody (mouse/human)
TAB-086MZ Anti-Human CTLA4 Recombinant Antibody (TGN2422.C) ELISA, FC Chimeric antibody (mouse/human)
TAB-085MZ-S(P) Anti-Human CTLA4 Recombinant Antibody scFv Fragment (TGN2122.C) ELISA, FC Chimeric antibody (mouse/human)
TAB-085MZ-F(E) Anti-Human CTLA4 Recombinant Antibody Fab Fragment (TGN2122.C) ELISA, FC Chimeric antibody (mouse/human)
TAB-086MZ-F(E) Anti-Human CTLA4 Recombinant Antibody Fab Fragment (TGN2422.C) ELISA, FC Chimeric antibody (mouse/human)

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0855 Hi-Affi™ Rabbit Anti-CTLA4 Recombinant Antibody (clone DS855AB) WB Rabbit IgG

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-067 Afuco™ Anti-CTLA4 ADCC Recombinant Antibody (Ipilimumab), ADCC Enhanced FuncS, IF, Neut, ELISA, FC ADCC enhanced antibody
AFC-TAB-206 Afuco™ Anti-CTLA4 ADCC Recombinant Antibody (Ticilimumab (= Tremelimumab)), ADCC Enhanced IF, IP, Neut, FuncS, ELISA ADCC enhanced antibody

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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