Human Anti-CTLA4 Recombinant Antibody (clone 10D1) (CAT#: HPAB-0051-YC)

The human sequence antibody specifically binds to human CTLA-4 or the CTLA-4 on the cell surface of normal human T cell. Herein the antibody is useful for treating human diseases, infections and other conditions using these antibodies.

Host: Human

Isotype: Human IgG, κ

Applications: ELISA, Cyt, Block


Specific Inquiry
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FC

Figure 1 shows melanoma-specific antibody responses in animals treated with a melanoma vaccine and the anti CTLA-4 antibody 10D1.

Antibody reactivity in pooled plasma samples from treated animals was measured by flow cytometry.

FuncS

Figure 2 shows the pharmacokinetic profile of the anti CTLA-4 antibody 10D1 during chronic dosing of primates.

Antibody was administered on days 0, 28, 56.84 and 140 and plasma concentrations of 10D1 were analyzed by ELISA. The mean +/-SEM of six treated animals is shown.

FuncS

Figure 3 shows the pharmacokinetic profile of anti-CTLA-4 antibody 10D1 in prostate cancer patients treated with a single dose of 10D1 at day 0

Plasma concentration (ug/ml) is shown. The mean +/-SEM for 14 treated patients is shown.

BL

Figure 4 Ligand blocking by mAb 10D1. Inhibition of recombinant B7.1 (A) and B7.2 (B) binding to CTLA-4-expressing T cells.

B7-Ig fusion proteins were incubated with huCTLA4 transfectants in the presence of varying concentrations of 10D1 F(ab)2 or isotype control F(ab)2. The mouse anti-human CTLA-4 mAb BNI-3 was also included as a positive control. B7-Ig binding was detected using goat anti-human IgG (Fc-specific)-PE probe. The cell-associated fluorescence was determined by flow cytometry, and percent inhibition of binding was determined.

Davis, T., Lonberg, N., Korman, A., Blanset, S. L., Srinivasan, M., & Graziano, R. F. (2003). Activity and Safety of CTLA-4 Blockade. J Immunol, 171, 6251-6259.

BL

Figure 5 CTLA-4 blockade with mAb 10D1 enhances Ab response to a melanoma vaccine in monkeys

Groups of six cynomolgus monkeys were given s.c. injections of 5106 SKmel-GM cells alone (arrows) or in combination with a bolus i.v. administration of mAb 10D1 (10 mg/kg). Analysis of Ab responses to SKmel-3 cells by flow cytometry (A) or ELISA (B) was determined from plasma samples taken 2 wk after each immunization. The values represent the mean SE from plasma samples, 6/group, tested at a 1/1000 dilution for flow cytometry or 1/200 for ELISA. Statistically significant differences by the Student t test are noted; p= 0.05,p=0.01,p=0.005. C, The individual animal responses by flow cytometry or ELISA (pretreatment values subtracted) at day 97.

Davis, T., Lonberg, N., Korman, A., Blanset, S. L., Srinivasan, M., & Graziano, R. F. (2003). Activity and Safety of CTLA-4 Blockade. J Immunol, 171, 6251-6259.

FuncS

Figure 6 Ab responses to melanoma and nonmelanoma cell lines in animals treated with melanoma vaccine and mAb 10D1.

Pooled plasma from six cynomolgus monkeys given SKmel-3-GM-CSF vaccine and mAb 10D1 (day 41) was tested for reactivity to a variety of melanoma and nonmelanoma cell lines by flow cytometry (1/1000 dilution).p 0.05human melanoma cell binding vs human nonmelanoma cells.

Davis, T., Lonberg, N., Korman, A., Blanset, S. L., Srinivasan, M., & Graziano, R. F. (2003). Activity and Safety of CTLA-4 Blockade. J Immunol, 171, 6251-6259.

ADCC

Figure 7 CTLA-4 blockade enhances ADCC activity in response to the melanoma vaccine.

Pooled plasma samples from vaccinated animals with or without 10D1 mAb were taken prior to immunization (Pre) or 2 wk after the second immunization (day 41). The samples were tested for ADCC activity on three melanoma cell lines at a 1/500 dilution.

Davis, T., Lonberg, N., Korman, A., Blanset, S. L., Srinivasan, M., & Graziano, R. F. (2003). Activity and Safety of CTLA-4 Blockade. J Immunol, 171, 6251-6259.


Specifications

  • Immunogen
  • Cells in PBS and soluble fusion protein
  • Host Species
  • Human
  • Type
  • Human IgG, κ
  • Specificity
  • Human CTLA4
  • Species Reactivity
  • Human, Monkey
  • Clone
  • 10D1
  • Applications
  • ELISA, Cyt, Block

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • Cytotoxic T-Lymphocyte-Associated Protein 4; Insulin-Dependent Diabetes Mellitus 12; Celiac Disease 3; CTLA-4; CD152; Ligand And Transmembrane Spliced Cytotoxic T Lymphocyte Associated Antigen 4; Cytotoxic T Lymphocyte Associated Antigen 4 Short Spliced Form; Cytotoxic T-Lymphocyte-Associated Serine Esterase-4; Cytotoxic T-Lymphocyte-Associated Antigen 4; CD152 Isoform; CD152 Antigen; CELIAC3; IDDM12; ALPS5; GRD4; GSE; CD

Related Resources

  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "Clone 10D1"

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Human Antibody

CAT Product Name Application Type
TAB-067 Anti-Human CTLA4 Recombinant Antibody (Ipilimumab) WB, FuncS, IF, Neut, ELISA, FC, IP IgG1 - kappa
TAB-206 Anti-Human CTLA4 Recombinant Antibody (Ticilimumab (Tremelimumab)) WB, IF, IP, Neut, FuncS, ELISA, FC IgG2

Immunotoxin

CAT Product Name Application Type
AGTO-G015E Anti-CTLA4 immunotoxin (scFv)-PE Cytotoxicity assay, Function study
AGTO-G015S Anti-CTLA4 immunotoxin (scFv)-Sap Cytotoxicity assay, Function study
AGTO-G015M Anti-CTLA4 immunotoxin (scFv)-Mel Cytotoxicity assay, Function study

Rat Antibody

CAT Product Name Application Type
TAB-195CL Mouse Anti-CTLA4 Recombinant Antibody (TAB-195CL) ELISA Mouse IgG

Humanized Antibody

CAT Product Name Application Type
TAB-083MZ Anti-Human CTLA4 Recombinant Antibody (TGN2122.H) ELISA, FC Humanized antibody
TAB-084MZ Anti-Human CTLA4 Recombinant Antibody (TGN2422.H) ELISA, FC Humanized antibody
TAB-088MZ Human Anti-CTLA4 Recombinant Antibody (TAB-088MZ) ELISA Human IgG
TAB-091MZ Human Anti-CTLA4 Recombinant Antibody (TAB-091MZ) ELISA Human IgG1
TAB-092MZ Human Anti-CTLA4 Recombinant Antibody (TAB-092MZ) ELISA Human IgG1

Chimeric Antibody

CAT Product Name Application Type
TAB-085MZ Anti-Human CTLA4 Recombinant Antibody (TGN2122.C) ELISA, FC Chimeric antibody (mouse/human)
TAB-086MZ Anti-Human CTLA4 Recombinant Antibody (TGN2422.C) ELISA, FC Chimeric antibody (mouse/human)
TAB-085MZ-S(P) Anti-Human CTLA4 Recombinant Antibody scFv Fragment (TGN2122.C) ELISA, FC Chimeric antibody (mouse/human)
TAB-086MZ-S(P) Anti-Human CTLA4 Recombinant Antibody scFv Fragment (TGN2422.C) ELISA, FC Chimeric antibody (mouse/human)
TAB-085MZ-F(E) Anti-Human CTLA4 Recombinant Antibody Fab Fragment (TGN2122.C) ELISA, FC Chimeric antibody (mouse/human)

Mouse Antibody

CAT Product Name Application Type
TAB-087MZ-S(P) Mouse Anti-CTLA4 Recombinant Antibody; scFv Fragment (TAB-087MZ-S(P)) ELISA Mouse scFv
TAB-089MZ-S(P) Mouse Anti-CTLA4 Recombinant Antibody; scFv Fragment (TAB-089MZ-S(P)) ELISA Mouse scFv
TAB-090MZ-S(P) Mouse Anti-CTLA4 Recombinant Antibody; scFv Fragment (TAB-090MZ-S(P)) ELISA Mouse scFv
TAB-093MZ-S(P) Anti-Human CTLA4 Recombinant Antibody scFv Fragment (Mu-26B) ELISA
TAB-087MZ-F(E) Mouse Anti-CTLA4 Recombinant Antibody; Fab Fragment (TAB-087MZ-F(E)) ELISA Mouse Fab

Neutralizing Antibody

CAT Product Name Application Type
NEUT-567CQ Mouse Anti-CTLA4 Recombinant Antibody (clone 14D3) FC, FuncS, Neut, IP, WB Mouse IgG2a, κ
NEUT-569CQ Mouse Anti-CTLA4 Recombinant Antibody (clone AS32) WB, Neut Mouse IgG1
NEUT-570CQ Human Anti-CTLA4 Recombinant Antibody (clone YC030) Neut Human IgG1, κ
NEUT-572CQ Hamster Anti-Ctla4 Recombinant Antibody (clone UC10-4B9) FC, Block, ELISA, IP Hamster IgG
NEUT-580CQ Rat Anti-Ctla4 Recombinant Antibody (clone CBL855) FC, Neut, WB Rat IgG2

Blocking Antibody

CAT Product Name Application Type
NEUT-568CQ Mouse Anti-CTLA4 Recombinant Antibody (clone L3D10) FC, Block Mouse IgG1, κ
NEUT-571CQ Mouse Anti-CTLA4 Recombinant Antibody (clone BN13) Block, FC Mouse IgG2a
NEUT-577CQ Hamster Anti-Ctla4 Recombinant Antibody (clone MAB0835) Block, FC Hamster IgG
NEUT-578CQ Hamster Anti-Ctla4 Recombinant Antibody (clone RYNmCTLA-4) Block Hamster IgG
NEUT-579CQ Hamster Anti-Ctla4 Recombinant Antibody (clone 9H10) FC, Block Hamster IgG

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0855 Hi-Affi™ Rabbit Anti-CTLA4 Recombinant Antibody (clone DS855AB) WB Rabbit IgG

scFv Fragment Antibody

CAT Product Name Application Type
HPAB-0051-YC-S(P) Human Anti-CTLA4 Recombinant Antibody (clone 10D1); scFv Fragment ELISA, Cyt, Block Human scFv
HPAB-1097-FY-F(E) Hamster Anti-Ctla4 Recombinant Antibody (clone FY-002); scFv Fragment ELISA Hamster scFv
HPAB-1098-FY-F(E) Human Anti-CTLA4 Recombinant Antibody; Fab Fragment (HPAB-1098-FY-F(E)) ELISA Human Fab
HPAB-1099-FY-F(E) Human Anti-CTLA4 Recombinant Antibody; Fab Fragment (HPAB-1099-FY-F(E)) ELISA Human Fab
HPAB-1100-FY-F(E) Human Anti-CTLA4 Recombinant Antibody; Fab Fragment (HPAB-1100-FY-F(E)) ELISA Human Fab

Fab Fragment Antibody

CAT Product Name Application Type
HPAB-0157-FY-F(E) Human Anti-CTLA4 Recombinant Antibody; Fab Fragment (HPAB-0157-FY-F(E)) Activ Humanized Fab
HPAB-N0343-YC-F(E) Human Anti-CTLA4 Recombinant Antibody; Fab Fragment (HPAB-N0343-YC-F(E)) FC, ELISA Human Fab
HPAB-0749LY-F(E) Human Anti-CTLA4 Recombinant Antibody; Fab Fragment (HPAB-0749LY-F(E)) FuncS, ELISA Human Fab
HPAB-0750LY-F(E) Human Anti-CTLA4 Recombinant Antibody; Fab Fragment (HPAB-0750LY-F(E)) FuncS, ELISA Human Fab
HPAB-0751LY-F(E) Human Anti-CTLA4 Recombinant Antibody; Fab Fragment (HPAB-0751LY-F(E)) FuncS, ELISA Human Fab

Recombinant Antibody

CAT Product Name Application Type
HPAB-0753LY Human Anti-CTLA4 Recombinant Antibody (HPAB-0753LY) FuncS, ELISA Human IgG
HPAB-0754LY Human Anti-CTLA4 Recombinant Antibody (HPAB-0754LY) FuncS, ELISA Human IgG
HPAB-0755LY Human Anti-CTLA4 Recombinant Antibody (HPAB-0755LY) FuncS, ELISA Human IgG1
HPAB-0756LY Human Anti-CTLA4 Recombinant Antibody (HPAB-0756LY) FuncS, ELISA Human IgG
HPAB-0757LY Human Anti-CTLA4 Recombinant Antibody (HPAB-0757LY) FuncS, ELISA Human IgG

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-067 Afuco™ Anti-CTLA4 ADCC Recombinant Antibody (Ipilimumab), ADCC Enhanced FuncS, IF, Neut, ELISA, FC ADCC enhanced antibody
AFC-TAB-206 Afuco™ Anti-CTLA4 ADCC Recombinant Antibody (Ticilimumab (= Tremelimumab)), ADCC Enhanced IF, IP, Neut, FuncS, ELISA ADCC enhanced antibody

Single-domain Antibody

CAT Product Name Application Type
HPAB-AP860-YC Camelid Anti-CTLA4 Recombinant Antibody; VHH Fragment (HPAB-AP860-YC) ELISA, Block Camel VHH
HPAB-AP861-YC Camelid Anti-CTLA4 Recombinant Antibody; VHH Fragment (HPAB-AP861-YC) ELISA, Block Camel VHH
HPAB-AP862-YC Camelid Anti-CTLA4 Recombinant Antibody; VHH Fragment (HPAB-AP862-YC) ELISA, Block Camel VHH
HPAB-AP863-YC Camelid Anti-CTLA4 Recombinant Antibody; VHH Fragment (HPAB-AP863-YC) ELISA, Block Camel VHH
HPAB-AP864-YC Camelid Anti-CTLA4 Recombinant Antibody; VHH Fragment (HPAB-AP864-YC) ELISA, Block Camel VHH

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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