Mouse Anti-CTLA4 Recombinant Antibody (clone 9D2E9) (CAT#: ZG-0262U)
Mouse anti-CTLA4 antibody (clone 9D2E9) displayed high specificity against the CTLA4 protein of Human.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
Figure 1 Mouse Anti-CTLA4 Antibody (ZG-0262U) in WB
Positive WB detected in: CEM whole cell lysate, K562 whole cell lysate, Raji whole cell lysate. All lanes: CTLA4 antibody at 1: 1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 25, 7, 9, 20 KDa
Observed band size: 34 KDa
Exposure time: 5min
Figure 2 Mouse Anti-CTLA4 Antibody (ZG-0262U) in IHC
IHC image of ZG-0262U diluted at 1:500 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- Recombinant Human Cytotoxic T-lymphocyte protein 4 protein (37-162AA)
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human CTLA4
- Species Reactivity
- Human
- Clone
- 9D2E9
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Immune Dysregulation With Autoimmunity, Immunodeficiency, And Lymphoproliferation and Celiac Disease 3.
Product Property
- Purification
- Protein G purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:1000-1:5000, IHC: 1:200-1:1000
Target
- Alternative Names
- CD; GSE; GRD4; ALPS5; CD152; CTLA-4; IDDM12; CELIAC3; cytotoxic T-lymphocyte protein 4; celiac disease 3; cytotoxic T lymphocyte associated antigen 4 short spliced form; cytotoxic T-lymphocyte-associated serine esterase-4; insulin-dependent diabetes mellitus 12; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4
- Gene ID
- 1493
- UniProt ID
- P16410
Related Resources
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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TAB-067 | Anti-Human CTLA4 Recombinant Antibody (TAB-067) | WB, FuncS, IF, Neut, ELISA, FC, IP | IgG1 - kappa |
TAB-206 | Anti-Human CTLA4 Recombinant Antibody (Ticilimumab (Tremelimumab)) | WB, IF, IP, Neut, FuncS, ELISA, FC | IgG2 |
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