Mouse Anti-CTLA4 Recombinant Antibody (clone 9D2E9) (CAT#: ZG-0262U)

Mouse anti-CTLA4 antibody (clone 9D2E9) displayed high specificity against the CTLA4 protein of Human.

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WB

Figure 1 Mouse Anti-CTLA4 Antibody (ZG-0262U) in WB

Figure 1 Mouse Anti-CTLA4 Antibody (ZG-0262U) in WB

Positive WB detected in: CEM whole cell lysate, K562 whole cell lysate, Raji whole cell lysate. All lanes: CTLA4 antibody at 1: 1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 25, 7, 9, 20 KDa
Observed band size: 34 KDa
Exposure time: 5min

IHC

Figure 2 Mouse Anti-CTLA4 Antibody (ZG-0262U) in IHC

Figure 2 Mouse Anti-CTLA4 Antibody (ZG-0262U) in IHC

IHC image of ZG-0262U diluted at 1:500 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.


Specifications

  • Immunogen
  • Recombinant Human Cytotoxic T-lymphocyte protein 4 protein (37-162AA)
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1
  • Specificity
  • Human CTLA4
  • Species Reactivity
  • Human
  • Clone
  • 9D2E9
  • Applications
  • ELISA, WB, IHC
  • Conjugate
  • Unconjugated
  • Related Disease
  • Immune Dysregulation With Autoimmunity, Immunodeficiency, And Lymphoproliferation and Celiac Disease 3.

Product Property

  • Purification
  • Protein G purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Buffer
  • PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
  • Preservative
  • 0.03% Proclin 300
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
    WB:1:1000-1:5000, IHC: 1:200-1:1000

Target

  • Alternative Names
  • CD; GSE; GRD4; ALPS5; CD152; CTLA-4; IDDM12; CELIAC3; cytotoxic T-lymphocyte protein 4; celiac disease 3; cytotoxic T lymphocyte associated antigen 4 short spliced form; cytotoxic T-lymphocyte-associated serine esterase-4; insulin-dependent diabetes mellitus 12; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4

Related Resources

  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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