Mouse Anti-CTLA4 Recombinant Antibody (clone 9D2E9)

Name: Mouse Anti-CTLA4 Recombinant Antibody (clone 9D2E9)
SKU: ZG-0262U
Rating: 5 (1 reviews)

CAT#: ZG-0262U

Mouse anti-CTLA4 antibody (clone 9D2E9) displayed high specificity against the CTLA4 protein of Human.

Tested Data Gene Expression

Figure 1 Mouse Anti-CTLA4 Antibody (ZG-0262U) in WB

Positive WB detected in: CEM whole cell lysate, K562 whole cell lysate, Raji whole cell lysate. All lanes: CTLA4 antibody at 1: 1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 25, 7, 9, 20 KDa
Observed band size: 34 KDa
Exposure time: 5min

IHC

Figure 2 Mouse Anti-CTLA4 Antibody (ZG-0262U) in IHC

IHC image of ZG-0262U diluted at 1:500 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

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RNA Expression Figure 1 RNA cell line category: Cell line enhanced (Karpas-707, SK-MEL-30, U-266/70, U-266/84)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000163599-CTLA4

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Specifications

Immunogen
Recombinant Human Cytotoxic T-lymphocyte protein 4 protein (37-162AA)

Host Species
Mouse

Type
Mouse IgG1

Specificity
Human CTLA4

Species Reactivity
Human

Clone
9D2E9

Applications
ELISA, WB, IHC

Conjugate
Unconjugated

Related Disease
Immune Dysregulation With Autoimmunity, Immunodeficiency, And Lymphoproliferation and Celiac Disease 3.

Product Property

Purification
Protein G purified

Purity
>95% as determined by SDS-PAGE

Buffer
PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.

Preservative
0.03% Proclin 300

Storage
Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:1000-1:5000, IHC: 1:200-1:1000

Target

Alternative Names
CD; GSE; GRD4; ALPS5; CD152; CTLA-4; IDDM12; CELIAC3; cytotoxic T-lymphocyte protein 4; celiac disease 3; cytotoxic T lymphocyte associated antigen 4 short spliced form; cytotoxic T-lymphocyte-associated serine esterase-4; insulin-dependent diabetes mellitus 12; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4

Gene ID
1493

UniProt ID
P16410

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Related Diseases

Rheumatoid Arthritis

Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - ZG-0262U.pdf

MSDS

MSDS - ZG-0262U.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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