Rabbit Anti-DKK1 Recombinant Antibody (clone R07-6J6) (CAT#: VS3-XY3332)

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human and Rat DKK1

• Species Reactivity
• Human, Rat

• Clone
• R07-6J6

• Applications
• WB, IHC-P, ICC, IF, FC

• Related Disease
• Chiari Malformation Type I; Osteoporosis, Juvenile

• Clonality
• Monoclonal

• Aggregation
• < 5%

• Format
• Liquid

• Buffer
• PBS (pH 7.4), 150 mM NaCl and 50% glycerol

• Preservative
• 0.02% sodium azide

• Storage
• Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• WB: 1/500-1/1000
  IHC: 1/50-1/100
  IF: 1/50-1/200
  FC: 1/50-1/100

• Alternative Names
• SK; DKK-1

• Gene ID
• 22943

• UniProt ID
• O94907

• Long Name
• Dickkopf WNT signaling pathway inhibitor 1

• Cellular Localization
• Secreted

• Post Translation Modifications
• Glycosylation at Ser61, Thr155, Ser163, Thr164, Ser169, Thr172, Thr173, Thr181, and Asn256 (NX_O94907 [NX_O94907-1])
  Modification sites at PhosphoSitePlus (O94907)
  Glycosylation from GlyGen (O94907) 18 sites, 1 O-linked glycan (13 sites)

• Protein Refseq
• NP_036374.1

• Function
• Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

Secondary Antibody

• 2RD-A1473-YJ
• Goat Anti-Rabbit IgG-F(ab')2 Secondary antibody