Does the clone 61B recognize a specific epitope?

Yes, the monoclonal antibody 61B reacts with a specific epitope of DLL4 (Delta-like 4). It is a recombinant human antibody that recognizes the human DLL4 protein. It has been reported for use in ELISA and Immunofluorescence applications.

What is the purity of this DLL4 antibody?

This antibody is highly pure, with greater than 95% purity as determined by both SDS-PAGE and HPLC analysis. It is a recombinant human IgG that has been validated for specificity against DLL4, ensuring clean results in your immunological assays.

Human Anti-DLL4 Recombinant Antibody (clone 61B)

CAT#: HPAB-1108-FY

This product is a recombinant antibody that recognizes DLL4. The monoclonal antibody 61B reacts with the epitope of DLL4.

Gene Expression

Normal Tissue Figure 1 IHC staining of human small intestine

Immunohistochemical staining of human small intestine shows strong membranous positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23392/51479_A_4_2_selected.jpg

Normal Tissue Figure 2 Colon

Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23392/51479_A_9_3.jpg

Normal Tissue Figure 3 Liver

Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23392/51479_A_8_4.jpg

Normal Tissue Figure 4 Kidney

Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23392/51479_A_8_5.jpg

Normal Tissue Figure 5 Testis

Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23392/51479_A_4_6.jpg

RNA Expression Figure 6 RNA cell line category: Group enriched (BJ hTERT+, HUVEC TERT2, SCLC-21H)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000128917-DLL4

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Specifications

Host Species
Human

Type
Human IgG

Specificity
Human DLL4

Species Reactivity
Human

Clone
61B

Applications
ELISA

Product Property

Purity
>95% as determined by SDS-PAGE and HPLC analysis

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
The DLL4 antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Immunofluorescence.

Target

Alternative Names
Delta Like Canonical Notch Ligand 4; Drosophila Delta Homolog 4; Delta4; Delta-Like 4 Homolog (Drosophila); Delta-Like 4 (Drosophila); Delta-Like 4 Homolog; Delta-Like 4 Protein; Delta-Like Protein 4

Gene ID
54567

UniProt ID
Q9NR61

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Breast cancer biomarkers at key points during disease progression

Specific Epitope Recognition

The clone 61B antibody showed excellent specificity for the DLL4 epitope in our ELISA assays. We used it to map interactions within the Notch signaling pathway. The recombinant human IgG format was stable and performed consistently across multiple plates, which is essential for our quantitative analysis.

High Purity Validated By HPLC

We prioritize reagent quality, and this antibody met our standards with greater than 95% purity determined by HPLC. This high level of purity resulted in very clean data for our immunofluorescence experiments. It specifically stained DLL4 without significant background, validating its utility for imaging.

Q&As

Does the clone 61B recognize a specific epitope?

A: Yes, the monoclonal antibody 61B reacts with a specific epitope of DLL4 (Delta-like 4). It is a recombinant human antibody that recognizes the human DLL4 protein. It has been reported for use in ELISA and Immunofluorescence applications.
What is the purity of this DLL4 antibody?

A: This antibody is highly pure, with greater than 95% purity as determined by both SDS-PAGE and HPLC analysis. It is a recombinant human IgG that has been validated for specificity against DLL4, ensuring clean results in your immunological assays.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - HPAB-1108-FY.pdf

MSDS

MSDS - HPAB-1108-FY.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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