Rabbit Anti-F3 Recombinant Antibody (clone 8B10) (CAT#: ZG-0661J)
This product is a rabbit antibody that recognizes F3.
Specific Inquiry
WB
Figure 1 Rabbit Anti-F3 Antibody (ZG-0661J) in WB
Western Blot
Positive WB detected in: HL60 whole cell lysate, U251 whole cell lysate, Hela whole cell lysate, NTERA-2 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate, Rat brain tissue
All lanes: F3 antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 34, 28 kDa
Observed band size: 46 kDa
IHC
Figure 2 Rabbit Anti-F3 Antibody (ZG-0661J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human Tissue Factor
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human F3
- Species Reactivity
- Human, Rat
- Clone
- 8B10
- Applications
- ELISA, WB, IHC
- Conjugate
- Uconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- Coagulation Factor III, Tissue Factor; Coagulation Factor III (Thromboplastin, Tissue Factor); Tissue Factor; TF; Coagulation Factor III; Thromboplastin; CD142 Antigen; CD142; TFA; CNTO-859; CNTO 859
- Gene ID
- 2152
- UniProt ID
- P13726
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1092z | Mouse Anti-F3 Recombinant Antibody (clone 18C8) | WB, FC, ICC, FuncS | Mouse IgG1 |
| PABZ-043 | Mouse Anti-F3 Recombinant Antibody (clone 5G9) | Block | Mouse IgG |
| PABW-043 | Human Anti-F3 Recombinant Antibody (clone D3H44) | WB, IHC, IF, FuncS | Humanized IgG |
| MOB-2336MZ | Recombinant Mouse Anti-Human F3 Antibody (clone 3L2) | ELISA, ICC/IF, IHC-Fr, IHC-P, IHC-P, WB | Mouse antibody |
| VS3-FY2093 | Rabbit Anti-F3 Recombinant Antibody (clone R01-8E5) | WB, IHC-Fr, IHC-P, IF, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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