Rabbit Anti-F3 Recombinant Antibody (clone R01-8E5) (CAT#: VS3-FY2093)
The R01-8E5 is a rabbit recombinant monoclonal antibody that is direct against human F3. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. We verify the QC result of the antibody through western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20).
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
ICC
Figure 1 Anti-F3 Antibody (VS3-FY2093) in ICC.
Immunocytochemistry staining of Tissue Factor (green) in SKOV-3 using the anti-Tissue Factor antibody. Nuclear DNA was labeled with DAPI (shown in blue).
WB
Figure 2 Anti-F3 Antibody (VS3-FY2093) in WB.
The Tissue Factor expression in A549, HL-60, U2OS lysates were detected using the anti-Tissue Factor by western blot assay.
Specifications
- Immunogen
- Recombinant human tissue factor protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human F3
- Species Reactivity
- Human
- Clone
- R01-8E5
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 33 kDa, while the observed molecular weight stands at 45 kDa.
- Related Disease
- Disseminated Intravascular Coagulation and Carotid Artery Thrombosis
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Coagulation Factor III, Tissue Factor; Coagulation Factor III (Thromboplastin, Tissue Factor); Tissue Factor; TF; Coagulation Factor III; Thromboplastin; CD142 Antigen; CD142; TFA
- Gene ID
- 2152
- UniProt ID
- P13726
- Sequence Similarities
- Belongs to the tissue factor family.
- Cellular Localization
- Membrane, Secreted
- Protein Refseq
- NP_001171567.1; NP_001984.1
- Function
- Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1092z | Mouse Anti-F3 Recombinant Antibody (clone 18C8) | WB, FC, ICC, FuncS | Mouse IgG1 |
| PABL-471 | Human Anti-F3 Recombinant Antibody (clone 10H10) | WB, IF, FuncS | Human IgG |
| MOB-2336MZ | Recombinant Mouse Anti-Human F3 Antibody (clone 3L2) | ELISA, ICC/IF, IHC-Fr, IHC-P, IHC-P, WB | Mouse antibody |
| FN-224CQ | Mouse Anti-F3 Recombinant Antibody (clone HTF-1) | FC, FuncS, ICC, IF, WB | Mouse IgG1, κ |
| VS3-FY530 | Recombinant Rabbit Anti-F3 Antibody (clone R04-7G2) | WB, IHC-P | Rabbit IgG |
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