Rabbit Anti-F3 Recombinant Antibody (clone R01-8E5) (CAT#: VS3-FY2093)

The R01-8E5 is a rabbit recombinant monoclonal antibody that is direct against human F3. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. We verify the QC result of the antibody through western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20).


Specific Inquiry
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  • Tested Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
ICC

Figure 1 Anti-F3 Antibody (VS3-FY2093) in ICC.

Figure 1 Anti-F3 Antibody (VS3-FY2093) in ICC.

Immunocytochemistry staining of Tissue Factor (green) in SKOV-3 using the anti-Tissue Factor antibody. Nuclear DNA was labeled with DAPI (shown in blue).

WB

Figure 2 Anti-F3 Antibody (VS3-FY2093) in WB.

Figure 2 Anti-F3 Antibody (VS3-FY2093) in WB.

The Tissue Factor expression in A549, HL-60, U2OS lysates were detected using the anti-Tissue Factor by western blot assay.


Specifications

  • Immunogen
  • Recombinant human tissue factor protein
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human F3
  • Species Reactivity
  • Human
  • Clone
  • R01-8E5
  • Applications
  • Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
  • MW
  • The calculated weight of the target protein is approximately 33 kDa, while the observed molecular weight stands at 45 kDa.
  • Related Disease
  • Disseminated Intravascular Coagulation and Carotid Artery Thrombosis

Product Property

  • Purification
  • Affinity Purified
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
  • Preservative
  • 0.01% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry: 1/50-1/100
    Immunofluorescence: 1/50-1/200
    Immunoprecipitation: 1/20

Target

  • Alternative Names
  • Coagulation Factor III, Tissue Factor; Coagulation Factor III (Thromboplastin, Tissue Factor); Tissue Factor; TF; Coagulation Factor III; Thromboplastin; CD142 Antigen; CD142; TFA
  • Sequence Similarities
  • Belongs to the tissue factor family.
  • Cellular Localization
  • Membrane, Secreted
  • Function
  • Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

Recommended Products

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "F3"

Recombinant Antibody

Deglycosylated Antibody (Non-glycosylated IgGs)

CAT Product Name Application Type
Gly-178LC Recombinant Anti-Human F3 Antibody (Non-glycosylated) ELISA Human antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1226 Hi-Affi™ Rabbit Anti-F3 Recombinant Antibody (clone DS1226AB) WB, IHC-P, ICC, IF Rabbit IgG

Human Antibody

CAT Product Name Application Type
TAB-473CQ Anti-Human F3 Recombinant Antibody (Tisotumab) ELISA, IHC, FC, IP, IF, Inhib IgG1, κ

Epitope-Specific Antibody

CAT Product Name Application Type
EPAF-0573CQ Mouse Anti-F3 Recombinant Antibody (clone hATR-5) ELISA, Inhib Mouse IgG4

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-473CQ Afuco™ Anti-F3 ADCC Recombinant Antibody (Tisotumab), ADCC Enhanced ELISA, IHC, FC, IP, IF, Inhib ADCC enhanced antibody

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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