Rabbit Anti-F3 Recombinant Antibody (clone R01-8E5)
CAT#: VS3-FY2093
The R01-8E5 is a rabbit recombinant monoclonal antibody that is direct against human F3. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF, IP. We verify the QC result of the antibody through western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49292/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line HaCaT
Immunofluorescent staining of human cell line HaCaT shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69132/1343_C8_1_selected.jpg
Subcellular Location
Figure 3 IHC staining of human nasopharynx
Immunohistochemical staining of human nasopharynx shows strong cytoplasmic positivity in respiratory epithelial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9438/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9438/27291_B_7_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9438/27291_A_6_6.jpg
Normal Tissue
Figure 6 Placenta
Decidual cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9438/27291_A_2_7.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (A-431, HaCaT, HBEC3-KT, U-2197)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000117525-F3
❮
❯
ICC
Figure 1 Anti-F3 Antibody (VS3-FY2093) in ICC.
Immunocytochemistry staining of Tissue Factor (green) in SKOV-3 using the anti-Tissue Factor antibody. Nuclear DNA was labeled with DAPI (shown in blue).
WB
Figure 2 Anti-F3 Antibody (VS3-FY2093) in WB.
The Tissue Factor expression in A549, HL-60, U2OS lysates were detected using the anti-Tissue Factor by western blot assay.
❮
❯
Specifications
- Immunogen
- Recombinant human tissue factor protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human F3
- Species Reactivity
- Human
- Clone
- R01-8E5
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 33 kDa, while the observed molecular weight stands at 45 kDa.
- Related Disease
- Disseminated Intravascular Coagulation and Carotid Artery Thrombosis
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Coagulation Factor III, Tissue Factor; Coagulation Factor III (Thromboplastin, Tissue Factor); Tissue Factor; TF; Coagulation Factor III; Thromboplastin; CD142 Antigen; CD142; TFA
- Gene ID
- 2152
- UniProt ID
- P13726
- Sequence Similarities
- Belongs to the tissue factor family.
- Cellular Localization
- Membrane, Secreted
- Protein Refseq
- NP_001171567.1; NP_001984.1
- Function
- Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-FY2093. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "F3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-258CL | Human Anti-F3 Recombinant Antibody (TAB-258CL) | ELISA | Human IgG |
| TAB-276CQ-F(E) | Human Anti-F3 Recombinant Antibody; Fab Fragment (TAB-276CQ-F(E)) | ELISA, FC | Humanized Fab |
| TAB-278CQ-F(E) | Human Anti-F3 Recombinant Antibody; Fab Fragment (TAB-278CQ-F(E)) | ELISA, FC | Humanized Fab |
| PABX-180 | Recombinant Human Anti-TF Antibody (D3H44) | WB, ELISA, FuncS | IgG |
| PABX-180-F (E) | Recombinant Human Anti-TF Antibody Fab Fragment (D3H44) | WB, ELISA, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-468 | Human Anti-F3 Recombinant Antibody (clone 10H10); Fab Fragment | WB, IF, FuncS | Human Fab |
| HPAB-0113-YC-F(E) | Human Anti-F3 Recombinant Antibody; Fab Fragment (HPAB-0113-YC-F(E)) | ELISA, FC, Neut | Human Fab |
| HPAB-0114-YC-F(E) | Mouse Anti-F3 Recombinant Antibody; Fab Fragment (HPAB-0114-YC-F(E)) | ELISA | Mouse Fab |
| HPAB-0117-YC-F(E) | Mouse Anti-F3 Recombinant Antibody (clone H36.D2.B7); Fab Fragment | ELISA, WB | Mouse Fab |
| PFBC-450 | Mouse Anti-F3 Recombinant Antibody Fab Fragment (PFBC-450) | ELISA, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBZ-043 | Mouse Anti-F3 Recombinant Antibody (clone 5G9); scFv Fragment | Block | Mouse scFv |
| PSBL-468 | Human Anti-F3 Recombinant Antibody (clone 10H10); scFv Fragment | WB, IF, FuncS | Human scFv |
| HPAB-0175CQ-S(P) | Human Anti-F3 Recombinant Antibody; scFv Fragment (HPAB-0175CQ-S(P)) | ELISA, WB, Inhib | Humanized scFv |
| PSBC-450 | Mouse Anti-F3 Recombinant Antibody scFv Fragment (PSBC-450) | ELISA, FuncS | Mouse scFv |
| HPAB-1000WJ-S(P) | Human Anti-F3 Recombinant Antibody (clone JT C5); scFv Fragment | ELISA, Inhib | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-277CQ-F(E) | Mouse Anti-F3 Recombinant Antibody; Fab Fragment (TAB-277CQ-F(E)) | ELISA, FC | Mouse Fab |
| PABX-179 | Recombinant Mouse Anti-TF Antibody (D3) | WB, ELISA, IF, FuncS | IgG |
| PABX-177-S (P) | Recombinant Human Anti-TF Antibody scFv Fragment (D3h44) | WB, ELISA, Neut, FuncS | scFv |
| PABX-179-S (P) | Recombinant Mouse Anti-TF Antibody scFv Fragment (D3) | WB, ELISA, IF, FuncS | scFv |
| PABX-179-F (E) | Recombinant Mouse Anti-TF Antibody Fab Fragment (D3) | WB, ELISA, IF, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-178LC | Recombinant Anti-Human F3 Antibody (Non-glycosylated) | ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0373MZ | Recombinant Mouse Anti-Human Tissue Factor Antibody (clone BIUG-5265) | RIA, WB, ELISA | Mouse antibody |
| HPAB-0113-YC | Human Anti-F3 Recombinant Antibody (HPAB-0113-YC) | ELISA, FC, Neut | Human IgG1 |
| HPAB-J0088-YC | Human Anti-F3 Recombinant Antibody (HPAB-J0088-YC) | ELISA, FC, IHC, FuncS | Human IgG1 |
| HPAB-J0089-YC | Human Anti-F3 Recombinant Antibody (HPAB-J0089-YC) | ELISA, FC, IHC, FuncS | Human IgG1 |
| ZG-0661J | Rabbit Anti-F3 Recombinant Antibody (clone 8B10) | ELISA, WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1226 | Hi-Affi™ Rabbit Anti-F3 Recombinant Antibody (clone DS1226AB) | WB, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-473CQ | Anti-Human F3 Recombinant Antibody (TAB-473CQ) | ELISA, IHC, FC, IP, IF, Inhib | IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-0573CQ | Mouse Anti-F3 Recombinant Antibody (clone hATR-5) | ELISA, Inhib | Mouse IgG4 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-473CQ | Afuco™ Anti-F3 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-473CQ) | ELISA, IHC, FC, IP, IF, Inhib | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1301 | AbPlus™ Anti-F3 Magnetic Beads (VS-0724-YC1301) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY78 | Mouse Anti-NHP F3 Recombinant Antibody (clone TF9-10H10) | ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY92 | Mouse Anti-F3 (clone H36.D2.B7) scFv-Fc Chimera | ELISA, Inhib | Mouse IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC631 | Recombinant Anti-F3 Vesicular Antibody, EV Displayed (VS-0425-YC631) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2412 | Anti-F3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2413 | Anti-Mouse F3 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: WB, ELISA, FC, IP, FuncS, IF, Neut
Application: WB, IF, FuncS
Application: ELISA
Application: FC, ADCC, CDC, Inhib
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, IF, Block, FuncS
Application: Neut, ELISA, IF, IP, FuncS, FC
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
