Human Anti-F8 Recombinant Antibody (clone Krix-1) (CAT#: VS-0524-YC28)
KRIX-1 is an anti-human factor VIII antibody. The antibody bound to full-length light chain, to fragments corresponding to A3C1, C1C2, and the isolated C1 domain. In contrast, KRIX-1 did not bind to the C1 or C1C2 domains with the substitution Arg2150His and did not inhibit the activity of Factor VIII molecule carrying the mutation Arg2150His. KRIX-1 inhibited the binding of Factor VIII to von Willebrand factor in a dose-dependent manner. KRIX-1 inhibits significantly the binding of Factor VIII to phospholipids but brings about incomplete inhibition even when added in large excess.
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Normal Tissue
RNA Expression
Figure 1 Colon
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18777/41664_A_7_3.jpg
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18777/41664_A_7_3.jpg
Figure 2 Kidney
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18777/41664_A_7_5.jpg
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18777/41664_A_7_5.jpg
Figure 3 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18777/41664_A_4_6.jpg
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18777/41664_A_4_6.jpg
Figure 4 RNA cell line category:Cell line enhanced (U-2197)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000185010-F8
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000185010-F8
Specifications
- Immunogen
- Human Factor VIII
- Host Species
- Human
- Derivation
- Hybridoma
- Type
- Human IgG
- Specificity
- Human factor VIII
- Species Reactivity
- Human
- Clone
- Krix-1
- Applications
- ELISA, Inhib, IP
- Related Disease
- Hemophilia A and Thrombophilia, X-Linked, Due To Factor Viii Defect.
Product Property
- Purification
- Protein A purified
- Purity
- >95%
- Buffer
- PBS, pH 7.4
- Preservative
- No preservative
- Storage
- Centrifuge briefly prior to opening vial. Store at 4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Native KRIX-1 inhibited up to 90% of Factor VIII activity.
Target
- Alternative Names
- Coagulation Factor VIII; Coagulation Factor VIII, Procoagulant Component; Antihemophilic Factor; F8C; AHF; Coagulation Factor VIII A1 Domain; Coagulation Factor VIII C2 Domain; Coagulation Factor VIIIc; Procoagulant Component;
- Gene ID
- 2157
- UniProt ID
- P00451
- Long Name
- Coagulation factor VIII
(FVIII)
- Sequence Similarities
- Belongs to the multicopper oxidase family.
- Cellular Localization
- Secreted, extracellular space
- Post Translation Modifications
- Sulfation on Tyr-1699 is essential for binding vWF.
Proteolytically cleaved by cathepsin CTSG to produce a partially activated form.
Glycosylation at Asn60, Asn258, Asn601, Asn776, Asn803, Asn847, Asn919, Asn962, Asn982, Asn1020, Asn1024, Asn1074, Asn1085, Asn1204, Asn1274, Asn1278, Asn1301, Asn1319, Asn1431, Asn1461, Asn1829, and Asn2137.
- Function
- Acts as a cofactor for F9/factor IXa when it converts F10/factor X to the activated form, factor Xa.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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|---|---|---|---|
| NAB-930-VHH | Recombinant Anti-Human F8 VHH Single Domain Antibody | ELISA, Neut, ICC, FC | Llama VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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