Human Anti-F8 Recombinant Antibody (clone Krix-1)
CAT#: VS-0524-YC28
KRIX-1 is an anti-human factor VIII antibody. The antibody bound to full-length light chain, to fragments corresponding to A3C1, C1C2, and the isolated C1 domain. In contrast, KRIX-1 did not bind to the C1 or C1C2 domains with the substitution Arg2150His and did not inhibit the activity of Factor VIII molecule carrying the mutation Arg2150His. KRIX-1 inhibited the binding of Factor VIII to von Willebrand factor in a dose-dependent manner. KRIX-1 inhibits significantly the binding of Factor VIII to phospholipids but brings about incomplete inhibition even when added in large excess.
Gene Expression
Normal Tissue
Figure 1 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18777/41664_A_7_3.jpg
Normal Tissue
Figure 2 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18777/41664_A_7_5.jpg
Normal Tissue
Figure 3 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18777/41664_A_4_6.jpg
RNA Expression
Figure 4 RNA cell line category:Cell line enhanced (U-2197)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000185010-F8
❮
❯
Specifications
- Immunogen
- Human Factor VIII
- Host Species
- Human
- Derivation
- Hybridoma
- Type
- Human IgG
- Specificity
- Human factor VIII
- Species Reactivity
- Human
- Clone
- Krix-1
- Applications
- ELISA, Inhib, IP
- Related Disease
- Hemophilia A and Thrombophilia, X-Linked, Due To Factor Viii Defect.
Product Property
- Purification
- Protein A purified
- Purity
- >95%
- Buffer
- PBS, pH 7.4
- Preservative
- No preservative
- Storage
- Centrifuge briefly prior to opening vial. Store at 4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Native KRIX-1 inhibited up to 90% of Factor VIII activity.
Target
- Alternative Names
- Coagulation Factor VIII; Coagulation Factor VIII, Procoagulant Component; Antihemophilic Factor; F8C; AHF; Coagulation Factor VIII A1 Domain; Coagulation Factor VIII C2 Domain; Coagulation Factor VIIIc; Procoagulant Component;
- Gene ID
- 2157
- UniProt ID
- P00451
- Long Name
- Coagulation factor VIII
(FVIII)
- Sequence Similarities
- Belongs to the multicopper oxidase family.
- Cellular Localization
- Secreted, extracellular space
- Post Translation Modifications
- Sulfation on Tyr-1699 is essential for binding vWF.
Proteolytically cleaved by cathepsin CTSG to produce a partially activated form.
Glycosylation at Asn60, Asn258, Asn601, Asn776, Asn803, Asn847, Asn919, Asn962, Asn982, Asn1020, Asn1024, Asn1074, Asn1085, Asn1204, Asn1274, Asn1278, Asn1301, Asn1319, Asn1431, Asn1461, Asn1829, and Asn2137.
- Function
- Acts as a cofactor for F9/factor IXa when it converts F10/factor X to the activated form, factor Xa.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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