CytoStream™ Rabbit Anti-GSK3B Recombinant Antibody (VS13-YC477)
CAT#: VS13-YC477
The CytoStream™ Rabbit Anti-GSK3B Recombinant Antibody is specific for human, mouse and rat GSK3B. GSK3B, a kinase subfamily member, regulates glucose homeostasis and stress responses, linked to diseases like Parkinson's and Alzheimer's through its metabolic and signaling activities. The anti-GSK3B antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28017/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16263/36255_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16263/36255_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16263/36255_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16263/36255_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16263/36255_A_7_8.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000082701-GSK3B
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Specifications
- Immunogen
- Recombinant human GSK3 beta, aa 360-400.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat GSK3B
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC-P, FC
- Conjugate
- NONE
- Related Disease
- Parkinson disease and Alzheimer disease
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
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Related Signaling Pathways
BCR Signaling Pathway
Canonical Wnt Signaling Pathway
ErbB Signaling Pathway
JAK-STAT Signaling Pathway
mTOR Signaling Pathway
Non-Canonical Wnt Signaling Pathway
PI3K-Akt Signaling Pathway
Related Diseases
Breast Cancer
Endometrial Cancer
Pancreatic Cancer
Prostate Cancer
Throid Cancer
Hepatocellular Carcinoma
Insulin Resistance
Cushing Syndrome
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "GSK3B"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0238MZ | Chicken Anti-GSK3b Polyclonal IgY | Indirect ELISA, WB | Chicken antibody |
| BRD-0751MZ | Chicken Anti-GSK3 beta Polyclonal IgY | ELISA, IHC, IP, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1526 | Rabbit Anti-GSK3B Recombinant Antibody (clone DS1526AB) | ICC, IHC-P, WB | Rabbit IgG |
| MOR-4682 | Rabbit Anti-GSK3B Recombinant Antibody (clone TH196DS) | WB, IHC-P, ChIP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1915-CN | Rabbit Anti-GSK3B Polyclonal Antibody (MRO-1915-CN) | IF, IHC, FC | Rabbit IgG |
| VS3-QX481 | Mouse Anti-GSK3B Recombinant Antibody (clone 4C4-3H9-10C5) | WB, IHC | Mouse IgG1 |
| VS3-QX482 | Mouse Anti-GSK3B Recombinant Antibody (clone 9B1-5C3-5G8) | WB, IHC | Mouse IgG1 |
| VS3-QX483 | Mouse Anti-GSK3B Recombinant Antibody (clone 9F9-5G6-7G2) | WB, IHC | Mouse IgG1 |
| VS3-XY783 | Mouse Anti-GSK3B Recombinant Antibody (clone 7D8A6) | ELISA, WB, IHC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1320 | AbPlus™ Anti-Gsk3b Magnetic Beads (VS-0724-YC1320) | IP, Protein Purification | |
| VS-0724-YC1321 | AbPlus™ Anti-GSK3B Magnetic Beads (VS-0724-YC1321) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY236 | Mouse Anti-NHP GSK3B Recombinant Antibody (clone 3D10) | WB, IHC, IF, FC, ELISA | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY960 | Anti-GSK3B Immunohistochemistry Kit | IHC | |
| VS-0525-XY2945 | Anti-Mouse GSK3B Immunohistochemistry Kit | IHC | |
| VS-0525-XY2944 | Anti-Human GSK3B Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC478 | CytoStream™ Mouse Anti-GSK3B Recombinant Antibody (VS13-YC478) | WB, IHC, FC | Mouse IgG1 |
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