Mouse Anti-NHP GSK3B Recombinant Antibody (clone 3D10)
CAT#: VS-1024-XY236
This mouse monoclonal antibody is specifically crafted to detect GSK3B, with proven reactivity to human, mouse, non-human primate, and rat samples. Demonstrating versatility across Western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), flow cytometry (FC), and ELISA, it ensures accurate and reliable analysis of GSK3B expression in a variety of research and diagnostic applications.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28017/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16263/36255_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16263/36255_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16263/36255_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16263/36255_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16263/36255_A_7_8.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000082701-GSK3B
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Specifications
- Immunogen
- Purified recombinant fragment of human GSK3B
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- GSK3B
- Species Reactivity
- Human, Mouse, Non-human primate, Rat
- Clone
- 3D10
- Applications
- WB, IHC, IF, FC, ELISA
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:1000, IHC: 1:200-1:800, IF: 1:200-1:800, FC: 1:200-1:400, ELISA: 1:8000
Target
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Related Signaling Pathways
BCR Signaling Pathway
Canonical Wnt Signaling Pathway
ErbB Signaling Pathway
JAK-STAT Signaling Pathway
mTOR Signaling Pathway
Non-Canonical Wnt Signaling Pathway
PI3K-Akt Signaling Pathway
Related Diseases
Breast Cancer
Endometrial Cancer
Pancreatic Cancer
Prostate Cancer
Throid Cancer
Hepatocellular Carcinoma
Insulin Resistance
Cushing Syndrome
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "GSK3B"
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| CAT | Product Name | Application | Type |
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| MOR-1526 | Rabbit Anti-GSK3B Recombinant Antibody (clone DS1526AB) | ICC, IHC-P, WB | Rabbit IgG |
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| CAT | Product Name | Application | Type |
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| MRO-0702-CN | Rabbit Anti-GSK3B Recombinant Antibody (clone CBACN-251) | WB, IF, IHC, FC | Rabbit IgG |
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| VS7-HM1050 | Mouse Anti-GSK3B Recombinant Antibody (clone CBL332HM) | WB, IHC-p, IF, ICC, FCM, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1320 | AbPlus™ Anti-Gsk3b Magnetic Beads (VS-0724-YC1320) | IP, Protein Purification | |
| VS-0724-YC1321 | AbPlus™ Anti-GSK3B Magnetic Beads (VS-0724-YC1321) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY960 | Anti-GSK3B Immunohistochemistry Kit | IHC | |
| VS-0525-XY2945 | Anti-Mouse GSK3B Immunohistochemistry Kit | IHC | |
| VS-0525-XY2944 | Anti-Human GSK3B Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC477 | CytoStream™ Rabbit Anti-GSK3B Recombinant Antibody (VS13-YC477) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
| VS13-YC478 | CytoStream™ Mouse Anti-GSK3B Recombinant Antibody (VS13-YC478) | WB, IHC, FC | Mouse IgG1 |
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