Mouse Anti-GSK3B Recombinant Antibody (ZG-0397J)
CAT#: ZG-0397J
This product is a mouse antibody that recognizes GSK3B.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-GSK3B Recombinant Antibody (ZG-0397J) in WB
Western blot analysis of extracts from A549, Hela, 3T3 and Rat Brain cell lysates using GSK-3β mouse mAb(1:1000 diluted). Predicted band size:46KDa.Observed band size:46KDa.
ICC
Figure 2 Mouse Anti-GSK3B Recombinant Antibody (ZG-0397J) in ICC
Immunocytochemistry staining of HeLa cells fixed with -20™Methanol and using GSK-3β mouse mAb (dilution 1:200).
IP
Figure 3 Mouse Anti-GSK3B Recombinant Antibody (ZG-0397J) in IP
Immunoprecipitation analysis of Hela cell lysates using GSK-3β.GSK-3β Rabbit mAb was used for the western blot analysis (1:1000 diluted).
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28017/if_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16263/36255_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16263/36255_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16263/36255_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16263/36255_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16263/36255_A_7_8.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000082701-GSK3B
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Specifications
- Immunogen
- Purified recombinant GSK-3β protein fragments expressed in E.coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human GSK3B
- Species Reactivity
- Human, Mouse
- Applications
- WB, ICC
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Western Blot, Immunocytochemistry.
Target
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Related Signaling Pathways
BCR Signaling Pathway
Canonical Wnt Signaling Pathway
ErbB Signaling Pathway
JAK-STAT Signaling Pathway
mTOR Signaling Pathway
Non-Canonical Wnt Signaling Pathway
PI3K-Akt Signaling Pathway
Related Diseases
Breast Cancer
Endometrial Cancer
Pancreatic Cancer
Prostate Cancer
Throid Cancer
Hepatocellular Carcinoma
Insulin Resistance
Cushing Syndrome
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| MRO-1915-CN | Rabbit Anti-GSK3B Polyclonal Antibody (MRO-1915-CN) | IF, IHC, FC | Rabbit IgG |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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