Afuco™ Anti-IL6R ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-032ML) (CAT#: AFC-TAB-032ML)

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• Tested Data
• Gene Expression
• Datasheet
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WB

Figure 1 Human Anti-Human IL6R Antibody (AFC-TAB-032ML) in WB

Western blot analysis of AFC-TAB-032ML was performed by loading Human IL6R Protein (His Tag) in reduced condition Lane 1, Lane 2, Lane 3 (0.1 μg, 0.3 μg, 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-453CT (2 μg/mL) and HRP-conjugated goat anti-human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.

DB

Figure 2 Human Anti-Human IL6R Antibody (AFC-TAB-032ML) in DB

Dot Blot analysis of AFC-TAB-032ML was performed by coating with Human IL6R Protein (His Tag).

AFC-TAB-032ML incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG as a secondary antibody (1: 2000)

ELISA

Figure 3 Human Anti-Human IL6R Antibody (AFC-TAB-032ML) in ELISA

ELISA analysis of AFC-TAB-032ML was performed by coating with Human IL6R Protein (His Tag). Then blocked with BSA and incubated with AFC-TAB-032ML. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

RNA Expression

Figure 1 RNA cell line category: Cell line enhanced (THP-1, U-266/84)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000160712-IL6R

Figure 1 RNA cell line category: Cell line enhanced (THP-1, U-266/84)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000160712-IL6R

 Datasheet

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.