Human Anti-MET Recombinant Antibody (clone 107_A07) (CAT#: PABC-457)
This product is a recombinant Human antibody that can recognize MET. The antibody, 107_A07, inhibited HGF/SF-induced cell migration and proliferation in vitro and inhibited growth of tumor xenografts in vivo. 107_A07 competes with both HGF/SF and its truncated splice variant NK1 for MET binding, despite the location of the antibody epitope on a domain (Ig1) not reported to bind NK1 or HGF/SF.
Specific Inquiry
BL
Figure 1 PEGylated 107A07 blocks angiogenesis in an in vitro angiogenesis assay in which co-culture with fbroblasts promotes endothelial cell sprouting and tubulogenesis.
(A) control culture, no Fab, (B) PEG-D1.3 Fab, (C) PEG-107_A07 Fab.
DiCara, D. M., Chirgadze, D. Y., Pope, A. R., Karatt-Vellatt, A., Winter, A., Slavny, P., ... & Mavria, G. (2017). Characterization and structural determination of a new anti-MET function-blocking antibody with binding epitope distinct from the ligand binding domain. Scientific reports, 7(1), 9000.
FuncS
Figure 2 Efect of 107_A07 IgG on the growth of subcutaneous U87MG xenografs.
Following xenograf implantation on day 0, mice (n=8) were given vehicle alone (PBS), control D1.3 IgG (10mg/kg) or 107_A07 IgG (2 or 10mg/kg) intraperitoneally at day 7 and then every 3–5 days for 26 days. Tumor volumes were measured by caliper twice weekly.
DiCara, D. M., Chirgadze, D. Y., Pope, A. R., Karatt-Vellatt, A., Winter, A., Slavny, P., ... & Mavria, G. (2017). Characterization and structural determination of a new anti-MET function-blocking antibody with binding epitope distinct from the ligand binding domain. Scientific reports, 7(1), 9000.
Specifications
- Host Species
- Human
- Derivation
- Human
- Type
- Human IgG1, κ
- Specificity
- Human MET
- Species Reactivity
- Human
- Clone
- 107_A07
- Applications
- Block, FuncS, ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Blocking, Functional Assay and Enzyme-linked Immunosorbent Assay. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. be carefully titrated in specific assays.
Target
Related Resources
EGFR Tyrosine Kinase Inhibitor Resistance
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G049E | Anti-MET immunotoxin (Fab)-PE | Cytotoxicity assay, Function study |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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