Mouse Anti-MYC Recombinant Antibody (clone 3D1E12)
CAT#: VS7-0425-WR448
This product is a mouse IgG2b antibody that binds to human MYC. The antibody 3D1E12 recognizes specific amino acid domain of human MYC (AA: 23-37). It can be used for FC assay.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55893/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line SK-MEL-30
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/66556/1279_E11_3_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows moderate nucleolar positivity in Purkinje cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55893/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human rectum
Immunohistochemical staining of human rectum shows moderate nuclear and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/84/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 5 IHC staining of human caudate
Immunohistochemical staining of human caudate shows distinct nucleolar positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 6 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55893/883_H10_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 7 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55893/879_G9_1_red_green.jpg
Normal Tissue
Figure 8 Cerebral cortex
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_B_7_5.jpg
Normal Tissue
Figure 9 Liver
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_A_9_4.jpg
Normal Tissue
Figure 10 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_A_7_5.jpg
Normal Tissue
Figure 11 Cervix
Squamous epithelial cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_B_8_3.jpg
Normal Tissue
Figure 12 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10307/30810_A_9_8.jpg
RNA Expression
Figure 13 RNA cell line category: Cell line enhanced (RPMI-8226)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136997-MYC
❮
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Specifications
- Immunogen
- Recombinant human MYC (AA: 23-37) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human MYC
- Species Reactivity
- Human
- Clone
- 3D1E12
- Molecule Class
- Proto-Oncogene
- Applications
- FC
- Conjugate
- Unconjugated
- Related Disease
- Burkitt lymphoma (BL)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- MYC; v-myc avian myelocytomatosis viral oncogene homolog; MRTL; MYCC; c-Myc; bHLHe39; myc proto-oncogene protein; proto-oncogene c-Myc; transcription factor p64; class E basic helix-loop-helix protein 39; avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog; myc-related translation/localization regulatory factor
- Gene ID
- 4609
- UniProt ID
- P01106
- Long Name
- MYC Proto-Oncogene, BHLH Transcription Factor
- Cellular Localization
- Cytoplasm; Nucleus
- Post Translation Modifications
- Acetylation; Glycoprotein; Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001341799.1
- Function
- Activator; DNA-binding
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Related Signaling Pathways
BCR Signaling Pathway
Related Diseases
Bladder Cancer
Breast Cancer
Colorectal Cancer
Gastric Cancer
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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Secondary Antibody
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Reconstitution Buffer
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